| Literature DB >> 29353476 |
Jin-Wei Zhou1,2, Huai-Zhi Luo1,2, Huan Jiang1,2, Ting-Kun Jian1,2, Zi-Qian Chen1,2, Ai-Qun Jia1.
Abstract
The quorum sensing (QS) inhibitory activity of hordenine from sprouting barley against foodborne pathogen Pseudomonas aeruginosa was evaluated for the first time here. At concentrations ranging from 0.5 to 1.0 mg mL-1, hordenine inhibited the levels of acyl-homoserine lactones. The enhanced susceptibility of hordenine with netilmicin on P. aeruginosa PAO1 biofilm formation as well as their efficiency in disrupting preformed biofilms was also evaluated using scanning electron microscopy and confocal laser scanning microscopy (CLSM). Hordenine treatment inhibited the production of QS-related extracellular virulence factors of P. aeruginosa PAO1. Additionally, quantitative real-time polymerase chain reaction analysis demonstrated that the expressions of QS-related genes, lasI, lasR, rhlI, and rhlR, were significantly suppressed. Our results indicated that hordenine can serve as a competitive inhibitor for signaling molecules and act as a novel QS-based agent to defend against foodborne pathogens.Entities:
Keywords: Pseudomonas aeruginosa; biofilm; foodborne pathogen; hordenine; quorum sensing
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Year: 2018 PMID： 29353476 DOI： 10.1021/acs.jafc.7b05035
Source DB: PubMed Journal: J Agric Food Chem ISSN： 0021-8561 Impact factor: 5.279