| Literature DB >> 29341543 |
Jheng-Syong Wu1,2, Yung-Chin Chung1, Jun-Jei Chien1, Chien Chou1,3.
Abstract
A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed. (2018) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE).Keywords: aberrations; confocal fluorescence microscopy; optical heterodyne
Mesh：
Year: 2018 PMID： 29341543 DOI： 10.1117/1.JBO.23.1.010502
Source DB: PubMed Journal: J Biomed Opt ISSN： 1083-3668 Impact factor: 3.170