Jiancheng Wang1, Nengtai Ouyang2, Long Qu1, Tengfei Lin3, Xianglin Zhang1, Yaren Yu1, Chongfei Jiang1, Liling Xie1, Liping Wang4, Zhigui Wang5, Shuzhen Ren6, Shizhi Chen7, Jiang Huang8, Fang Liu9, Weiqing Huang10, Xianhui Qin1. 1. National Clinical Research Center for Kidney Disease; Renal Division, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China. 2. Cell Molecular Diagnostic Center, Department of Clinical Laboratory, Sun Yat-sen Memorial Hospital, the Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China. 3. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Key Laboratory for Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China. 4. Department of Gynecology, Shanxi Medical University, Taiyuan, Shanxi Province, China. 5. Department of Pathology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China. 6. Department of Clinical Laboratory, Pingtan Comprehensive Experimental Area Hospital, Fuzhou, Fujian Province, China. 7. Cell Molecular Diagnostic Center, Department of Clinical Laboratory, Second Hospital Affiliated of Chongqing Medical University, Chongqing, China. 8. Department of Cardiology, Xiangya Pingkuang Cooperation Hospital, Pingxiang, Jiangxi Province, China. 9. Department of Clinical Laboratory, West China Second University Hospital of Sichuan University, Chengdu, Sichuan Province, China. 10. Department of Pathology, Qingdao Municipal Hospital, Affiliated to Medical College of Qingdao University, Qingdao, Shandong Province, China.
Abstract
BACKGROUND AND OBJECTIVES: The Chinese population typically has inadequate folate intake and no mandatory folic acid fortification. Methylenetetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR) are the two key regulatory enzymes in the folate/homocysteine (Hcy) metabolism. Hcy has been implicated in the pathogenesis of cardiovascular disease. We conducted a meta-analysis to assess whether the MTHFR gene A1298C and the MTRR gene A66G polymorphisms affect Hcy levels in the Chinese population. METHODS: This analysis included 13 studies with Hcy levels reported as one of the study measurements. Summary estimates of weighted mean differences and 95% confidence intervals (CIs) were obtained using random-effect models. RESULTS: Overall, there were no significant differences in Hcy concentrations between participants with the MTHFR 1298 CC (12 trials, n = 129), AA (n = 2166; β, -0.51 μmol/L; 95%CI: -2.14, 1.11; P = 0.53), or AC genotype (n = 958; β, 0.55 μmol/L; 95%CI: -0.72, 1.82; P = 0.40). Consistently, compared to those with the MTRR 66 GG genotype (6 trials, n = 156), similar Hcy concentrations were found in participants with the AA (n = 832; β, -0.43 μmol/L; 95%CI: -1.04, 0.17; P = 0.16) or AG (n =743; β, -0.57 μmol/L; 95%CI: -1.46, 0.31; P = 0.21) genotype. Similar results were observed for the dominant and recessive models. CONCLUSIONS: Neither the MTHFR A1298C polymorphism nor the MTRR A66G polymorphism affects Hcy levels in the Chinese population.
BACKGROUND AND OBJECTIVES: The Chinese population typically has inadequate folate intake and no mandatory folic acid fortification. Methylenetetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR) are the two key regulatory enzymes in the folate/homocysteine (Hcy) metabolism. Hcy has been implicated in the pathogenesis of cardiovascular disease. We conducted a meta-analysis to assess whether the MTHFR gene A1298C and the MTRR gene A66G polymorphisms affect Hcy levels in the Chinese population. METHODS: This analysis included 13 studies with Hcy levels reported as one of the study measurements. Summary estimates of weighted mean differences and 95% confidence intervals (CIs) were obtained using random-effect models. RESULTS: Overall, there were no significant differences in Hcy concentrations between participants with the MTHFR 1298 CC (12 trials, n = 129), AA (n = 2166; β, -0.51 μmol/L; 95%CI: -2.14, 1.11; P = 0.53), or AC genotype (n = 958; β, 0.55 μmol/L; 95%CI: -0.72, 1.82; P = 0.40). Consistently, compared to those with the MTRR 66 GG genotype (6 trials, n = 156), similar Hcy concentrations were found in participants with the AA (n = 832; β, -0.43 μmol/L; 95%CI: -1.04, 0.17; P = 0.16) or AG (n =743; β, -0.57 μmol/L; 95%CI: -1.46, 0.31; P = 0.21) genotype. Similar results were observed for the dominant and recessive models. CONCLUSIONS: Neither the MTHFR A1298C polymorphism nor the MTRR A66G polymorphism affects Hcy levels in the Chinese population.
Authors: Daqing Zhang; Xiaohua Jiang; Pu Fang; Yan Yan; Jian Song; Sapna Gupta; Andrew I Schafer; William Durante; Warren D Kruger; Xiaofeng Yang; Hong Wang Journal: Circulation Date: 2009-10-26 Impact factor: 29.690