Jingzhi Chang1, Yuxia Zhang1, Yichuan Li1, Kun Lu1, Yongjie Shen1, Yali Guo1, Qingfeng Qi2, Mingchen Wang2, Shanfeng Zhang2. 1. Department of Biochemistry & Molecular Biology, ShangQiu Medical College, No. 486 Beihai West Road, Shangqiu 476100, PR China. 2. Department of Biochemistry & Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, Zhengzhou 450001, PR China.
Abstract
AIM: Though lutein can inhibit cancer cell proliferation via alleviating oxidative injury, the molecular mechanisms of lutein involvement in the NrF2/antioxidant response element (ARE) and NF-κB pathways remain poorly understood. MATERIALS & METHODS: MTT, flow cytometry, quantitative real-time PCR (qRT-PCR) and western blot assays were performed. RESULTS: After treatment with lutein, breast cancer cell proliferation was significantly decreased in a dose-dependent manner. Lutein induced nuclear translocation and protein expression of NrF2, improved the expression of cellular antioxidant enzymes and attenuated reactive oxygen species levels. Moreover, lutein treatment decreased NF-κB signaling pathway related NF-κB p65 protein expression. CONCLUSION: The effect of lutein antiproliferation was mediated by activation of the NrF2/ARE pathway, and blocking of the NF-κB signaling pathway.
AIM: Though lutein can inhibit cancer cell proliferation via alleviating oxidative injury, the molecular mechanisms of lutein involvement in the NrF2/antioxidant response element (ARE) and NF-κB pathways remain poorly understood. MATERIALS & METHODS:MTT, flow cytometry, quantitative real-time PCR (qRT-PCR) and western blot assays were performed. RESULTS: After treatment with lutein, breast cancer cell proliferation was significantly decreased in a dose-dependent manner. Lutein induced nuclear translocation and protein expression of NrF2, improved the expression of cellular antioxidant enzymes and attenuated reactive oxygen species levels. Moreover, lutein treatment decreased NF-κB signaling pathway related NF-κB p65 protein expression. CONCLUSION: The effect of lutein antiproliferation was mediated by activation of the NrF2/ARE pathway, and blocking of the NF-κB signaling pathway.
Authors: Camila Maria Simplicio-Revoredo; Renato de Oliveira Pereira; Mariella de Almeida Melo; Pedro Vitor Lopes-Costa; Paulo de Tarso Moura-Borges; Emerson Brandão Sousa; Fidelis Manes Neto; Viriato Campelo; Ione Maria Ribeiro Soares-Lopes; Maria da Conceição Barros-Oliveira; Cleciton Braga Tavares; Alesse Ribeiro Dos Santos; Camila Guedes Borges de Araújo; Eid Gonçalves Coelho; Larysse Cardoso Campos-Verdes; Aldenora Oliveira do Nascimento-Holanda; Jackeline Lopes Viana; Maria Liduina Meneses Bezerra-Chaves; Rodrigo José de Vasconcelos-Valença; Lina Gomes Dos Santos; Lauro Rodolpho Soares-Lopes; André Luiz Pinho-Sobral; Luiz Henrique Gebrim; Benedito Borges da Silva Journal: Oncotarget Date: 2020-05-05
Authors: Said Moshawih; Rabi'atul Nur Amalia Abdullah Juperi; Ganesh Sritheran Paneerselvam; Long Chiau Ming; Kai Bin Liew; Bey Hing Goh; Yaser Mohammed Al-Worafi; Chee-Yan Choo; Shobna Thuraisingam; Hui Poh Goh; Nurolaini Kifli Journal: Molecules Date: 2022-03-17 Impact factor: 4.411