Wenhua Xu1,2, Andrew B Hawkey3, Hui Li1, Lu Dai4, Howard H Brim1, Jacqueline A Frank1, Jia Luo1, Susan Barron3, Gang Chen1. 1. Department of Pharmacology & Nutritional Sciences, University of Kentucky College of Medicine, Lexington, Kentucky. 2. Department of Neurology, Affiliated Provincial Hospital of Anhui Medical University, Hefei, China. 3. Department of Psychology, University of Kentucky College of Art & Sciences, Lexington, Kentucky. 4. Department of Toxicology & Cancer Biology, University of Kentucky College of Medicine, Lexington, Kentucky.
Abstract
BACKGROUND: Fetal ethanol (EtOH) exposure can damage the developing central nervous system and lead to cognitive and behavioral deficits, known as fetal alcohol spectrum disorders (FASD). EtOH exposure to mouse pups during early neonatal development was used as a model of EtOH exposure that overlaps the human third-trimester "brain growth spurt"-a model that has been widely used to study FASD in rats. METHODS: C57BL/6 male and female mice were exposed to EtOH (4 g/kg/d) on postnatal days (PD) 4 to 10 by oral intubation. Intubated and nontreated controls were also included. Behavioral testing of the offspring, including open field, elevated plus maze, and Morris water maze, was performed on PD 20 to 45. RESULTS: EtOH exposure during PD 4 to 10 resulted in hyperactivity and deficits in learning and memory in young mice with no apparent sex differences. CONCLUSIONS: Based on these data, this neonatal intubation mouse model may be useful for future mechanistic and genetic studies of FASD and for screening of novel therapeutic agents.
BACKGROUND: Fetal ethanol (EtOH) exposure can damage the developing central nervous system and lead to cognitive and behavioral deficits, known as fetal alcohol spectrum disorders (FASD). EtOH exposure to mouse pups during early neonatal development was used as a model of EtOH exposure that overlaps the human third-trimester "brain growth spurt"-a model that has been widely used to study FASD in rats. METHODS: C57BL/6 male and female mice were exposed to EtOH (4 g/kg/d) on postnatal days (PD) 4 to 10 by oral intubation. Intubated and nontreated controls were also included. Behavioral testing of the offspring, including open field, elevated plus maze, and Morris water maze, was performed on PD 20 to 45. RESULTS:EtOH exposure during PD 4 to 10 resulted in hyperactivity and deficits in learning and memory in young mice with no apparent sex differences. CONCLUSIONS: Based on these data, this neonatal intubation mouse model may be useful for future mechanistic and genetic studies of FASD and for screening of novel therapeutic agents.
Authors: C Ikonomidou; P Bittigau; M J Ishimaru; D F Wozniak; C Koch; K Genz; M T Price; V Stefovska; F Hörster; T Tenkova; K Dikranian; J W Olney Journal: Science Date: 2000-02-11 Impact factor: 47.728
Authors: David F Wozniak; Richard E Hartman; Maureen P Boyle; Sherri K Vogt; Ashley R Brooks; Tatyana Tenkova; Chainllie Young; John W Olney; Louis J Muglia Journal: Neurobiol Dis Date: 2004-12 Impact factor: 5.996
Authors: Ilknur Dursun; Ewa Jakubowska-Doğru; Birsen Elibol-Can; Deborah van der List; Barbara Chapman; Lihong Qi; Robert F Berman Journal: Alcohol Date: 2013-02-08 Impact factor: 2.405
Authors: Belkis Jacquez; Hyesun Choi; Clark W Bird; David N Linsenbardt; C Fernando Valenzuela Journal: Behav Brain Res Date: 2020-08-26 Impact factor: 3.332