3-D mineralized silk fibroin/polycaprolactone composite scaffold modified with polyglutamate conjugated with BMP-2 peptide for bone tissue engineering.

In the field of bone tissue engineering, an ideal three-dimensional (3-D) scaffold should not only structurally mimic the extracellular matrix (ECM) in large tissues but also mechanically support the bone healing process and provide biochemical cues to induce osteogenesis. In this study, we investigated the feasibility of functionalisation of scaffolds by coupling polyglutamate acid conjugated with BMP-2 peptide onto silk fibroin (SF)/polycaprolactone (PCL) (SF/PCL) blend nanofibers. The morphology, composition, and mineralisation, were confirmed by FE-SEM, XRD, and FT-IR spectroscopy. The FE-SEM images revealed that wet-electrospun nanofibrous scaffolds exhibited inter-connected nano/micro-pores at different levels, and a different morphology was observed on the 3-D SF/PCL scaffold after mineralisation. Furthermore, the binding property and release behaviour of the peptide were investigated on this mineralized structure, and adipose-derived stem cells were seeded on the composite scaffolds to assay their cytocompatibility and osteogenic differentiation capacities. Results suggest that the polyglutamate motif (repetitive glutamate amino acids) exhibited markedly improved binding properties to mineralized nanofibers, and the mineralized 3-D scaffolds with the conjugated with peptide enhances the mRNA expression of osteogenic genes. The sponge-like 3-D nanofibrous scaffold mechanically and biochemically mimics the regenerative process for applications in bone tissue engineering, including the regeneration of calvarial defects.