Shigeng Zhang1, Qi Zhang2, Qing Sun3, Jinlong Tang4, Jimin Chen1, Na Ji5, Yichun Zheng1, Francia Fang6, Wanjun Lei7, Pengpeng Li7, Nan Zhang1. 1. Department of Surgical Urology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. 2. Department of Hepatobiliary and Pancreatic Surgery, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. 3. Department of Nutrient, Affiliated Hospital of Jilin Medical University, Jilin, China. 4. Department of Pathology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. 5. Department of Anesthesiology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. 6. Trinity College of Arts and Sciences, Duke University, Durham, North Carolina, USA. 7. Beijing Novogene Bioinformatics Technology Co., Ltd, Beijing, China.
Abstract
BACKGROUND/AIMS: Malignant mesothelioma of the tunica vaginalis testis is a rare and lethal disease. The genomic characteristics and genetic changes of tumor cells during the progression of this disease are unknown. METHODS: we performed whole-genome sequencing of four successive tumor samples derived from surgery and a blood sample in a single patient. RESULTS: All tumors were found to have significant C-to-T and T-to-C mutations, and amplification of copy number in chromosomes 1 and 12 were notified in all tumor samples. Subclone analysis revealed a parallel evolution of the tumor in this patient. We also identified some mutations in mesothelioma-associated genes such as KIF25, AHNAK, and PRDM2. CONCLUSIONS: The results showed a comprehensive genomic change in malignant mesothelioma of the tunica vaginalis testis and provide a better understanding of the clonal evolution during tumor recurrence and metastasis.
BACKGROUND/AIMS: Malignant mesothelioma of the tunica vaginalis testis is a rare and lethal disease. The genomic characteristics and genetic changes of tumor cells during the progression of this disease are unknown. METHODS: we performed whole-genome sequencing of four successive tumor samples derived from surgery and a blood sample in a single patient. RESULTS: All tumors were found to have significant C-to-T and T-to-C mutations, and amplification of copy number in chromosomes 1 and 12 were notified in all tumor samples. Subclone analysis revealed a parallel evolution of the tumor in this patient. We also identified some mutations in mesothelioma-associated genes such as KIF25, AHNAK, and PRDM2. CONCLUSIONS: The results showed a comprehensive genomic change in malignant mesothelioma of the tunica vaginalis testis and provide a better understanding of the clonal evolution during tumor recurrence and metastasis.
Authors: Luigi Vimercati; Domenica Cavone; Maria Celeste Delfino; Luigi De Maria; Antonio Caputi; Giovanni Maria Ferri; Gabriella Serio Journal: Environ Health Date: 2019-08-30 Impact factor: 5.984
Authors: Matthew H Bailey; William U Meyerson; Lewis Jonathan Dursi; Liang-Bo Wang; Guanlan Dong; Wen-Wei Liang; Amila Weerasinghe; Shantao Li; Yize Li; Sean Kelso; Gordon Saksena; Kyle Ellrott; Michael C Wendl; David A Wheeler; Gad Getz; Jared T Simpson; Mark B Gerstein; Li Ding Journal: Nat Commun Date: 2020-09-21 Impact factor: 14.919