Sayuri Udagawa1, Sayaka Katagiri1, Shogo Maekawa1, Yasuo Takeuchi1, Rina Komazaki1, Anri Ohtsu1, Naoki Sasaki1, Takahiko Shiba1, Kazuki Watanabe1, Kazuyuki Ishihara2,3, Noriko Sato4, Naoyuki Miyasaka5, Yuichi Izumi1. 1. a Department of Periodontology, Graduate School of Medical and Dental Sciences , Tokyo Medical and Dental University , Tokyo , Japan. 2. b Oral Health Science Center , Tokyo Dental College , Tokyo , Japan. 3. c Department of Microbiology , Tokyo Dental College , Tokyo , Japan. 4. d Department of Molecular Epidemiology , Medical Research Institute, Tokyo Medical and Dental University , Tokyo , Japan. 5. e Department of Comprehensive Reproductive Medicine , Tokyo Medical and Dental University , Tokyo , Japan.
Abstract
OBJECTIVE: Growing evidence indicates an association between periodontitis and delivery outcome; however, the mechanism is unclear. This study aimed to investigate the influence of Porphyromonas gingivalis (Pg) infection on delivery outcome in mice. MATERIALS AND METHODS: Bacteremia was induced in pregnant Slc:ICR mice (8 weeks old) by intravenous injection of Pg. Mice were randomly divided into a control group (CO), and those receiving Pg injection at gestational day 1 (GD1), gestational day 15 (GD15) or every day (ED). Delivery outcome, Pg infection, and gene expression in the placenta and umbilical cord were evaluated. RESULTS: Birth weight was lower in the ED and GD15 groups than in the CO group. A remarkable increase in anti-Pg IgG antibody was observed in the ED and GD1 groups, although Pg was not detected in the placenta or umbilical cord. mRNA expression of Tnfα and Il6 in the placenta, and Hif1α in the umbilical cord, was significantly increased in the ED group. Microarray analysis of the umbilical cord revealed increased expression of several genes including Orm1, Mgl2, Rps6ka3 and Trim15 in the ED group. CONCLUSIONS: Pg infection during the third trimester caused low birth weight and inflammation in the placenta and umbilical cord.
OBJECTIVE: Growing evidence indicates an association between periodontitis and delivery outcome; however, the mechanism is unclear. This study aimed to investigate the influence of Porphyromonas gingivalis (Pg) infection on delivery outcome in mice. MATERIALS AND METHODS:Bacteremia was induced in pregnant Slc:ICR mice (8 weeks old) by intravenous injection of Pg. Mice were randomly divided into a control group (CO), and those receiving Pg injection at gestational day 1 (GD1), gestational day 15 (GD15) or every day (ED). Delivery outcome, Pg infection, and gene expression in the placenta and umbilical cord were evaluated. RESULTS: Birth weight was lower in the ED and GD15 groups than in the CO group. A remarkable increase in anti-Pg IgG antibody was observed in the ED and GD1 groups, although Pg was not detected in the placenta or umbilical cord. mRNA expression of Tnfα and Il6 in the placenta, and Hif1α in the umbilical cord, was significantly increased in the ED group. Microarray analysis of the umbilical cord revealed increased expression of several genes including Orm1, Mgl2, Rps6ka3 and Trim15 in the ED group. CONCLUSIONS:Pg infection during the third trimester caused low birth weight and inflammation in the placenta and umbilical cord.
Authors: Gerson Aparecido Foratori-Junior; Bruna Machado da Silva; Ana Carolina da Silva Pinto; Heitor Marque Honório; Francisco Carlos Groppo; Silvia Helena de Carvalho Sales-Peres Journal: Clin Oral Investig Date: 2019-05-08 Impact factor: 3.573
Authors: Luis Daniel Sansores-España; Samanta Melgar-Rodríguez; Katherine Olivares-Sagredo; Emilio A Cafferata; Víctor Manuel Martínez-Aguilar; Rolando Vernal; Andrea Cristina Paula-Lima; Jaime Díaz-Zúñiga Journal: Front Aging Date: 2021-12-10