Literature DB >> 29330797

Testing Acute Oxygen Sensing in Genetically Modified Mice: Plethysmography and Amperometry.

Patricia Ortega-Sáenz1,2,3, Candela Caballero4,5,6,7, Lin Gao4,5,6, José López-Barneo8,9,10.   

Abstract

Monitoring responsiveness to acute hypoxia of whole animals and single cells is essential to investigate the nature of the mechanisms underlying oxygen (O2) sensing. Here we describe the protocols followed in our laboratory to evaluate the ventilatory response to hypoxia in normal and genetically modified animals. We also describe the amperometric technique used to monitor single-cell catecholamine release from chemoreceptor cells in carotid body and adrenal medulla slices.

Entities:  

Keywords:  Acute oxygen sensing; Amperometry; Genetically modified mice; Hyperventilation; Hypoxia; Plethysmography; Single-cell secretion

Mesh：

Substances：

Year:  2018        PMID： 29330797     DOI： 10.1007/978-1-4939-7665-2_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Measuring Breathing Patterns in Mice Using Whole-body Plethysmography.

Authors:  Patricia Prada-Dacasa; Andrea Urpi; Laura Sánchez-Benito; Patrizia Bianchi; Albert Quintana
Journal:  Bio Protoc       Date:  2020-09-05

2.  Loss of Cervical Sympathetic Chain Input to the Superior Cervical Ganglia Affects the Ventilatory Responses to Hypoxic Challenge in Freely-Moving C57BL6 Mice.

Authors:  Paulina M Getsy; Gregory A Coffee; Yee-Hsee Hsieh; Stephen J Lewis
Journal:  Front Physiol       Date:  2021-04-22       Impact factor: 4.566

3.  Using redox-sensitive fluorescent probes to record real-time reactive oxygen species production in cells from mouse carotid body slices.

Authors:  Lin Gao; Ignacio Arias-Mayenco; Patricia Ortega-Sáenz; José López-Barneo
Journal:  STAR Protoc       Date:  2021-05-11

4.  Ventilatory responses during and following hypercapnic gas challenge are impaired in male but not female endothelial NOS knock-out mice.

Authors:  Paulina M Getsy; Sripriya Sundararajan; Walter J May; Graham C von Schill; Dylan K McLaughlin; Lisa A Palmer; Stephen J Lewis
Journal:  Sci Rep       Date:  2021-10-18       Impact factor: 4.379

5.  Oxygen regulation of breathing is abolished in mitochondrial complex III-deficient arterial chemoreceptors.

Authors:  Daniel Cabello-Rivera; Patricia Ortega-Sáenz; Lin Gao; Ana M Muñoz-Cabello; Victoria Bonilla-Henao; Paul T Schumacker; José López-Barneo
Journal:  Proc Natl Acad Sci U S A       Date:  2022-09-19       Impact factor: 12.779

6.  Lactate sensing mechanisms in arterial chemoreceptor cells.

Authors:  Hortensia Torres-Torrelo; Patricia Ortega-Sáenz; Lin Gao; José López-Barneo
Journal:  Nat Commun       Date:  2021-07-06       Impact factor: 14.919

7.  Short-term facilitation of breathing upon cessation of hypoxic challenge is impaired in male but not female endothelial NOS knock-out mice.

Authors:  Paulina M Getsy; Sripriya Sundararajan; Walter J May; Graham C von Schill; Dylan K McLaughlin; Lisa A Palmer; Stephen J Lewis
Journal:  Sci Rep       Date:  2021-09-15       Impact factor: 4.379
  7 in total

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