Vitor Mendonça Alves1, Fernanda Bernadelli De Vito2, Paulo Roberto Juliano Martins3, Sheila Soares Silva4, Lilian Castilho5, Helio Moraes-Souza6. 1. Universidade Federal do Triângulo Mineiro (UFTM) Avenida Getúlio Guaritá, 159 - Abadia, Uberaba, MG, 38025-440, Brazil. Electronic address: vitor.bio.uftm@bol.com.br. 2. Universidade Federal do Triângulo Mineiro (UFTM) Avenida Getúlio Guaritá, 159 - Abadia, Uberaba, MG, 38025-440, Brazil. Electronic address: fernandabernadelli@yahoo.com.br. 3. Universidade Federal do Triângulo Mineiro (UFTM) Avenida Getúlio Guaritá, 159 - Abadia, Uberaba, MG, 38025-440, Brazil; Hemocentro Regional de Uberaba/Fundação HEMOMINAS Avenida Getúlio Guaritá, 250 - Abadia, Uberaba, MG, 38025-440, Brazil. Electronic address: paulo.martins@hemominas.mg.gov.br. 4. Universidade Federal do Triângulo Mineiro (UFTM) Avenida Getúlio Guaritá, 159 - Abadia, Uberaba, MG, 38025-440, Brazil; Hemocentro Regional de Uberaba/Fundação HEMOMINAS Avenida Getúlio Guaritá, 250 - Abadia, Uberaba, MG, 38025-440, Brazil. Electronic address: sheila.silva@uftm.edu.br. 5. Hemocentro da Universidade Estadual de Campinas (UNICAMP), Rua Carlos Chagas, 480 - Cidade Universitária, Campinas, SP, 13083-878, Brazil. Electronic address: castilho@unicamp.br. 6. Universidade Federal do Triângulo Mineiro (UFTM) Avenida Getúlio Guaritá, 159 - Abadia, Uberaba, MG, 38025-440, Brazil; Hemocentro Regional de Uberaba/Fundação HEMOMINAS Avenida Getúlio Guaritá, 250 - Abadia, Uberaba, MG, 38025-440, Brazil. Electronic address: helio.moraes@uftm.edu.br.
Abstract
BACKGROUND AND OBJECTIVES: The frequency of red blood cell (RBC) antigens in Brazil varies due to differences in the ethnic groups in different regions; however, these studies have not been performed in Minas Gerais, where African admixture is more prevalent in comparison with other states. Due to these facts, this study aimed to determine the frequency of RBC genotypes on Rh, Kell, Duffy and Kidd systems in blood donors and multi-transfused patients from Minas Gerais, Southeast Brazil. METHODS: Blood samples were collected from 170 donors and 117 patients with different diagnosis and at least three RBC transfusions. DNA was extracted from leukocytes and genotyped by PCR-SSP, Multiplex or RFLP to alleles of the referred systems. The results were compared by the Chi-Square test, with a significance level of 5%. RESULTS: The most frequent genotypes were: RHD+, RHCE*ce/RHCE*ce, KEL*2/KEL*2, FY*B-67T/FY*B-67T and JK*A/JK*B. FY*B-67C/FY*B-67C, RHD*Ψ and JK*A/JK*A genotypes were more prevalent in sickle cell disease (SCD) patients than in donors. Many differences in RBC genotype frequencies were observed in comparison with studies from other states and countries. CONCLUSION: The results reinforce the importance of determining RBC genotypes of blood donors and patients in different regions of Brazil and the world, improving the transfusion safety of individuals requiring chronic RBC transfusions, especially those with SCD, due to ethnic differences in relation to donors.
BACKGROUND AND OBJECTIVES: The frequency of red blood cell (RBC) antigens in Brazil varies due to differences in the ethnic groups in different regions; however, these studies have not been performed in Minas Gerais, where African admixture is more prevalent in comparison with other states. Due to these facts, this study aimed to determine the frequency of RBC genotypes on Rh, Kell, Duffy and Kidd systems in blood donors and multi-transfused patients from Minas Gerais, Southeast Brazil. METHODS: Blood samples were collected from 170 donors and 117 patients with different diagnosis and at least three RBC transfusions. DNA was extracted from leukocytes and genotyped by PCR-SSP, Multiplex or RFLP to alleles of the referred systems. The results were compared by the Chi-Square test, with a significance level of 5%. RESULTS: The most frequent genotypes were: RHD+, RHCE*ce/RHCE*ce, KEL*2/KEL*2, FY*B-67T/FY*B-67T and JK*A/JK*B. FY*B-67C/FY*B-67C, RHD*Ψ and JK*A/JK*A genotypes were more prevalent in sickle cell disease (SCD) patients than in donors. Many differences in RBC genotype frequencies were observed in comparison with studies from other states and countries. CONCLUSION: The results reinforce the importance of determining RBC genotypes of blood donors and patients in different regions of Brazil and the world, improving the transfusion safety of individuals requiring chronic RBC transfusions, especially those with SCD, due to ethnic differences in relation to donors.