Qin Gong1,2, Xianjie Wen3, Heng Li4, Jian He3, Yunhua Wang3, Huiping Wu3, Hanbing Wang3, Xiaoping Wang1. 1. a Department of Pain Management , The First Affiliated Hospital of Jinan University , Guangzhou , Guangdong Province , China. 2. b Department of Anesthesiology , Foshan Hospital of Traditional Chinese Medicine , Foshan , Guangdong Province , China. 3. c Department of Anesthesiology , The First People's Hospital of Foshan & Foshan Hospital of Sun Yat-sen University , Foshan , Guangdong Province , China. 4. d Department of Anesthesiology , The Sixth Affiliated Hospital of Guangzhou Medical University , Qinyuan , Guangdong Province , China.
Abstract
BACKGROUND: Neurotoxicity induced by the local anaesthetics has aroused concern. A previous study has shown that an overload of intracellular calcium was involved in the neurotoxic effect. Cav3.1 is one of the low-voltage-activated (LVA) calcium channels which play a key point to regulate the intracellular calcium ion level. This study aimed to investigate the changes of the Cav3.1 expression in the SH-SY5Y cells treated with lidocaine hydrochloride. METHODS: The SH-SY5Y cells were treated with different concentrations of lidocaine hydrochloride(1 mM, 5 mM and 10 mM, namely L1 group, L5 group and L10 group) and different exposure times (1 h,12 h and 24 h), respectively. Cell viability, Cav3.1 protein and mRNA expression were detected. RESULTS: The results showed that cell viability decreased and Cav3.1 mRNA and protein expression increased with the concentration (from 1 mM to 10 mM) of the lidocaine hydrochloride and exposure time (from 1 h to 24 h) to the SH-SY5Y cell line increased. CONCLUSION: Those data showed that lidocaine hydrochloride induced SH-SY5Y cell toxicity and up-regulated Cav3.1mRNA and protein expression.
BACKGROUND:Neurotoxicity induced by the local anaesthetics has aroused concern. A previous study has shown that an overload of intracellular calcium was involved in the neurotoxic effect. Cav3.1 is one of the low-voltage-activated (LVA) calcium channels which play a key point to regulate the intracellular calcium ion level. This study aimed to investigate the changes of the Cav3.1 expression in the SH-SY5Y cells treated with lidocaine hydrochloride. METHODS: The SH-SY5Y cells were treated with different concentrations of lidocaine hydrochloride(1 mM, 5 mM and 10 mM, namely L1 group, L5 group and L10 group) and different exposure times (1 h,12 h and 24 h), respectively. Cell viability, Cav3.1 protein and mRNA expression were detected. RESULTS: The results showed that cell viability decreased and Cav3.1 mRNA and protein expression increased with the concentration (from 1 mM to 10 mM) of the lidocaine hydrochloride and exposure time (from 1 h to 24 h) to the SH-SY5Y cell line increased. CONCLUSION: Those data showed that lidocaine hydrochloride induced SH-SY5Y cell toxicity and up-regulated Cav3.1mRNA and protein expression.
Entities:
Keywords:
Cav3.1; Lidocaine hydrochloride; SH-SY5Y cells; local anaesthetics; neurotoxicity