Arzu Keskin-Aktan1, Kazime Gonca Akbulut2, Çiğdem Yazici-Mutlu3, Gizem Sonugur4, Müge Ocal5, Hakan Akbulut6. 1. Department of Physiology, Gazi University School of Medicine, Ankara, Turkey. 2. Department of Physiology, Gazi University School of Medicine, Ankara, Turkey. Electronic address: kgonca@gazi.edu.tr. 3. Department of Interdisciplinary Neuroscience, Health Science Institute, Ankara University, Ankara, Turkey. 4. Department of Internal Medicine, Ankara University School of Medicine, Ankara, Turkey. Electronic address: gizem.sonugur@ankara.edu.tr. 5. Department of Internal Medicine, Ankara University School of Medicine, Ankara, Turkey. Electronic address: mocal@ankara.edu.tr. 6. Department of Internal Medicine, Ankara University School of Medicine, Ankara, Turkey. Electronic address: akbulut@medicine.ankara.edu.tr.
Abstract
OBJECTIVE: Though the mechanisms are not clearly understood, melatonin and curcumin have been reported to have neuroprotective effects. However, the mechanisms of neuroprotective effects of melatonin and curcumin in the brain are not clearly understood. In the current study, we investigated the effects of melatonin and curcumin treatments on oxidative stress parameters, the expression of SIRT2, Bcl-2 and Bax in the hippocampus. METHODS: A total of thirty adult (13 months-old) male Wistar rats were divided into five groups: Control (1% ethanol:PBS), s.c. for 30 days), dimethyl sulfoxide (10%, s.c. for 30 days), Melatonin (10 mg/kg/day, s.c. for 30 days), Curcumin (30 mg/kg/day, i.p. for 30 days) and Salermide (100 μM, i.p. for 30 days). The levels of malondialdehyde (MDA) glutathione (GSH) were measured as oxidative stress parameters in the hippocampus. The expression levels of SIRT2, Bcl-2 and Bax proteins were tested by western blotting and the SIRT2 protein levels of the hippocampal region was measured by a sandwich ELISA method. RESULTS: Melatonin and curcumin significantly decreased MDA and SIRT2 expression in the hippocampus (p < 0.05). Accordingly, a significant increase in the GSH levels of curcumin-treated group and melatonin-treated group was observed. Melatonin, but not curcumin, significantly increased the Bcl-2 expression of the hippocampal region. There was a significant correlation between SIRT2 and MDA levels (p < 0.05). DISCUSSION: In conclusion, our results suggest that melatonin may increase cell survival in the hippocampus via decreasing oxidative stress and SIRT2 expression and increasing Bcl-2 expression.
OBJECTIVE: Though the mechanisms are not clearly understood, melatonin and curcumin have been reported to have neuroprotective effects. However, the mechanisms of neuroprotective effects of melatonin and curcumin in the brain are not clearly understood. In the current study, we investigated the effects of melatonin and curcumin treatments on oxidative stress parameters, the expression of SIRT2, Bcl-2 and Bax in the hippocampus. METHODS: A total of thirty adult (13 months-old) male Wistar rats were divided into five groups: Control (1% ethanol:PBS), s.c. for 30 days), dimethyl sulfoxide (10%, s.c. for 30 days), Melatonin (10 mg/kg/day, s.c. for 30 days), Curcumin (30 mg/kg/day, i.p. for 30 days) and Salermide (100 μM, i.p. for 30 days). The levels of malondialdehyde (MDA) glutathione (GSH) were measured as oxidative stress parameters in the hippocampus. The expression levels of SIRT2, Bcl-2 and Bax proteins were tested by western blotting and the SIRT2 protein levels of the hippocampal region was measured by a sandwich ELISA method. RESULTS:Melatonin and curcumin significantly decreased MDA and SIRT2 expression in the hippocampus (p < 0.05). Accordingly, a significant increase in the GSH levels of curcumin-treated group and melatonin-treated group was observed. Melatonin, but not curcumin, significantly increased the Bcl-2 expression of the hippocampal region. There was a significant correlation between SIRT2 and MDA levels (p < 0.05). DISCUSSION: In conclusion, our results suggest that melatonin may increase cell survival in the hippocampus via decreasing oxidative stress and SIRT2 expression and increasing Bcl-2 expression.
Authors: Dolly Patricia Pardo-Mora; Oscar Julián Murillo; Mauricio Rey-Buitrago; Mónica Losada-Barragán; Jaime Fabian Cruz Uribe; Karina Basso Santiago; Bruno José Conti; Eliza de Oliveira Cardoso; Fernanda Lopes Conte; Rafael María Gutiérrez; Orlando Torres García; José Maurício Sforcin Journal: Vet World Date: 2021-04-22