Chao Zhang1, Stephen D Campbell2, Sabine H Dickens3, Bin Yang2. 1. The State Key Laboratory Breeding Base of Basic Science of Stomatology, Hubei Province and Key Laboratory of Oral Biomedicine, Ministry of Education (Hubei-MOST KLOS & KLOBME), School and Hospital of Stomatology, Wuhan University, Wuhan, China. 2. Restorative Department, College of Dentistry, University of Illinois at Chicago, Chicago, IL. 3. American Dental Association Foundation, Paffenbarger Research Center, National Institute of Standards and Technology, Gaithersburg, MD.
Abstract
PURPOSE: To evaluate the remineralization of natural human dentin caries with an experimental whisker-reinforced Atraumatic Restorative Treatment (ART) composite. MATERIALS AND METHODS: Teeth with moderate active dentin caries were prepared with caries-disclosing dye and hand instruments, restored with ART or resin-modified glass ionomer cement (RM-GIC), and then wet sliced into 120-µm sections with 15 sections in each group. After taking transverse microradiographs and implementing digital image analysis to determine the "mineral-loss-before," each section was incubated in artificial saliva solution (pH = 7.0) for 4 weeks and 8 weeks with 1 hour each workday in demineralization solution (pH = 4.3). Transverse microradiographs of each section were retaken, and the "mineral-loss-after" was determined. The remineralization was calculated from [1-("mineral-loss-after"/"mineral-loss-before")] × 100%. Results were statistically analyzed with a repeated-measures ANOVA with one within-subject factor (time: 4 and 8 weeks) and one between-subject factor (material: ART and RM-GIC) (α = 0.05). RESULTS: The statistical analysis indicated that ART composite resulted in significantly higher remineralization than the RM-GIC (p ≤ 0.05). For the remineralization of each material, there was a statistical difference between 4 weeks and 8 weeks (p ≤ 0.05). CONCLUSIONS: This experimental ART composite remineralized natural human dentin caries better than the RM-GIC.
PURPOSE: To evaluate the remineralization of natural human dentin caries with an experimental whisker-reinforced Atraumatic Restorative Treatment (ART) composite. MATERIALS AND METHODS: Teeth with moderate active dentin caries were prepared with caries-disclosing dye and hand instruments, restored with ART or resin-modified glass ionomer cement (RM-GIC), and then wet sliced into 120-µm sections with 15 sections in each group. After taking transverse microradiographs and implementing digital image analysis to determine the "mineral-loss-before," each section was incubated in artificial saliva solution (pH = 7.0) for 4 weeks and 8 weeks with 1 hour each workday in demineralization solution (pH = 4.3). Transverse microradiographs of each section were retaken, and the "mineral-loss-after" was determined. The remineralization was calculated from [1-("mineral-loss-after"/"mineral-loss-before")] × 100%. Results were statistically analyzed with a repeated-measures ANOVA with one within-subject factor (time: 4 and 8 weeks) and one between-subject factor (material: ART and RM-GIC) (α = 0.05). RESULTS: The statistical analysis indicated that ART composite resulted in significantly higher remineralization than the RM-GIC (p ≤ 0.05). For the remineralization of each material, there was a statistical difference between 4 weeks and 8 weeks (p ≤ 0.05). CONCLUSIONS: This experimental ART composite remineralized natural human dentin caries better than the RM-GIC.