Dapagliflozin and Empagliflozin Ameliorate Hepatic Dysfunction Among Chinese Subjects with Diabetes in Part Through Glycemic Improvement: A Single-Center, Retrospective, Observational Study.

INTRODUCTION: Sodium-glucose co-transporter 2 inhibitors (SGLT2i) improve hepatic dysfunction, although studies focusing on their underlying mechanisms are lacking, especially ones on dapagliflozin and empagliflozin. Here, we investigated the relationship between amelioration of hepatic dysfunction and improvement in various metabolic parameters among Chinese subjects with type 2 diabetes (T2DM).
METHODS: This was a single-center, retrospective, observational study that involved 115 Chinese participants with T2DM treated with either dapagliflozin or empagliflozin for at least 6 months between July 2016 and February 2017.
RESULTS: Of the 115 participants included in this study, 69 received dapagliflozin and 46 received empagliflozin. After 6 months of treatment, all patients showed significant improvements in body weight (BW), systolic blood pressure (SBP) and fasting glucose (FG) and glycated hemoglobin (HbA1c) levels. All participants also showed a significant reduction in serum alanine aminotransferase (ALT) levels, from 40.3 ± 28.0 to 29.0 ± 14.1 U/L (p < 0.001). Pearson's correlation analysis revealed a positive correlation between the reduction in ALT levels after treatment with the respective SGLT2i and changes in FG (p = 0.014) and HbA1c (p = 0.043) levels over 6 months, but not with changes in BW and SBP. Multiple linear regression analysis revealed that the reduction in serum ALT levels was independently associated with changes in both HbA1c and FG but not with the changes in the other clinical variables, including BW.
CONCLUSIONS: Dapagliflozin and empagliflozin improved both metabolic and hepatic dysfunction as a class effect. The amelioration of hepatic dysfunction was mediated partly through an alleviation of hyperglycemia and possibly through an improvement in insulin resistance, independent of BW.

Introduction

Both non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM) are common health problems worldwide, including China [1, 2]. Strikingly, a recent study found that almost half of Chinese adults suffered from T2DM or prediabetes [2]. In another study, the prevalence of NAFLD was reported to be as high as 70% among Chinese subjects with T2DM [3]. Notably, the relationship between T2DM and NAFLD is bi-directional: the presence of NAFLD has been related to increased risk of all-cause mortality among subjects with T2DM [4], while T2DM is also a predictor for the development, progression and complications of NAFLD. T2DM has been found to increase the risk of cirrhosis [5], double the risk of hepatocellular carcinoma [6] and to be an independent predictor of overall and liver mortality in patients with NAFLD [4, 7]. Collectively, NAFLD has gradually emerged as an important diabetic complication.

The introduction of sodium–glucose co-transporter 2 inhibitors (SGLT2i) into clinical practice represents a novel approach to glycemic control in T2DM [8]. The benefits of this class of prescription medicines on cardiovascular and renal outcomes in patients with T2DM have been well demonstrated in large long-term randomized controlled trials [1, 9–11], as well as in a large-scale real world observational study [12]. A recent review also suggested that SGLT2i are anti-diabetic agents that should be considered in the treatment of subjects comorbid with T2DM and NAFLD [13]. Although a meta-analysis of 43 randomized controlled trials showed that SGLT2i decreases serum alanine aminotransferase (ALT) levels [14], the relationship between such ALT reductions and the improvement in metabolic parameters after the use of SGLT2i remains controversial. For example, a recent pooled analysis of data from six controlled studies on canagliflozin attributed the significant improvement in serum ALT levels to a combined reduction in glycated hemoglobin (HbA1c) levels and body weight (BW) reduction [15], while the authors of some animal and human studies of other SGLT2i attributed the improvement to mechanisms independent of BW reduction [16, 17]. Therefore, in order to examine whether SGLT2i ameliorate hepatic dysfunction through glycemic improvement and BW reduction as a class effect, we performed this retrospective observational study to investigate the relationship between improvement in hepatic dysfunction and metabolic parameters, in Chinese subjects with T2DM who were treated with either the SGLT2i dapagliflozin or empagliflozin.

Methods

Subjects

The study included subjects with T2DM who were followed up at the Diabetes Clinic of Queen Mary Hospital, Hong Kong. All subjects were on SGLT2i, either dapagliflozin or empagliflozin, between 1 July 2016 and 28 February 2017 and were identified through the computer-based clinical management system. Only those who had been prescribed SGLT2i (dapagliflozin or empagliflozin) for at least 6 months were included in this study. Participants who were known hepatitis B carriers were excluded from this study. This study is a clinical audit and does not involve any new studies of human subjects performed by any of the authors.

Design

This was a single-center, retrospective, observational study conducted at a university hospital in Hong Kong. This study was a clinical audit consequently does not involve any new studies of human subjects performed by any of the authors. Moreover, given the clinical audit nature of the study, informed consent was not obtained from the included participants; nonetheless all patient records and information were rendered anonymous prior to analysis. Clinical data, including demographics (age, sex and smoking habit), anthropometric parameters [BW, height, body mass index (BMI), systolic and diastolic blood pressure (SBP and DBP) measurements], comorbidities, metabolic parameters, including fasting glucose, lipid profile, HbA1c, and biochemical parameters, including serum creatinine (SCr), ALT, aspartate aminotransferase (AST) and albumin levels, were measured at baseline and after 26 weeks of SGLT2i use.

In this study, the estimated glomerular filtration rate (eGFR) was calculated using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation and expressed as eGFR = 141 × min (SCr/κ,1)α × max (SCr/κ,1)−1.209 × 0.993age (× 1.018 if female), where κ is 0.7 for females and 0.9 for males, α is − 0.329 for females and − 0.411 for males, min indicates the minimum of SCr/κ or 1 and max indicates the maximum of SCr/κ or 1 [18]. We defined hypertension as BP ≥ 140/90 mmHg or as being on anti-hypertensive medications.

Statistical Analysis

All data were analyzed using the IBM SPSS Statistics 24.0 program (IBM Corp., Armonk, NY). Values were reported as the mean ± standard deviation, median with interquartile range or percentage, as appropriate. Data for all continuous variables were tested for normality. Skewed variables and all delta changes were logarithmically transformed before analysis. Data at baseline and at 26 weeks after initiation of SGLT2i therapy were compared using paired Student’s t test and the Wilcoxon signed-rank test. Pearson’s correlations analysis was performed to determine the correlation of the reduction in serum ALT levels with baseline and changes of other variables after treatment with SGLT2i for 6 months. Multiple linear regression was performed to evaluate the independent variables that were associated with the reduction in serum ALT levels. One-way analysis of variance and the Chi-square test, or Fisher’s exact test, whichever was appropriate, were also employed to compare if there was any difference between the use of dapagliflozin and empagliflozin, as well as between groups with or without background insulin therapy. In all statistical tests, two-sided p values of < 0.05 were considered to be significant.

Results

SGLT2i Improved Metabolic Parameters Among Chinese Participants with T2DM

Of the 115 Chinese participants with T2DM included in this study, 69 and 46 were treated with dapagliflozin and empagliflozin, respectively. The mean age of the participants was 56.3 ± 11.1 years and mean duration of diabetes was 16.1 ± 8.0 years. Among them, 79.1% were non-drinkers or social drinkers, and 14.8% were ex-drinkers. With regard to background anti-diabetic agents before starting on SGLT2i, 110 (95.7%) participants were on metformin, 44 (38.3%) were on dipeptidyl-peptidase-4 inhibitors, 44 (38.3%) were on sulphonylureas, six (5.2%) were on glitazones, seven (6.1%) were on glucagon-like-peptide-1 receptor agonists and 66 (57.4%) were on insulin therapy (Electronic Supplementary Material Table 1). At baseline, their mean BMI was 30.5 ± 5.8 kg/m2, mean HbA1c was 8.6 ± 1.4% and fasting glucose was 9.1 ± 2.7 mmol/L. In addition, 102 (88.7%) participants had hypertension, 76 (66.1%) were on lipid-lowering medications and 32 (27.8%) had established cardiovascular disease.

After 6 months of treatment with SGLT2i, metabolic parameters, including BW, SBP, fasting glucose and HbA1c, had improved significantly (Table 1). The participants showed a weight loss of 1.6 kg [95% confidence interval (CI) − 2.2 to − 1.1; p < 0.001], reduction in SBP of 5.3 mmHg (95% CI − 9.1 to − 1.5; p = 0.006), fasting glucose of 1.9 mmol/L (95% CI − 2.5 to − 1.4; p < 0.001) and HbA1c of 1.0% (95% CI − 1.2 to − 0.8; p < 0.001), as well as significantly increased high-density lipoprotein and decreased eGFR, at 26 weeks (HDL: mean change 0.04 mmol/L, 95% CI 0.003–0.08, p = 0.033; eGFR: mean change − 1.6 ml/min/1.73 m2, 95% CI − 2.9 to − 0.3, p = 0.016). Notably, among the 66 participants on background insulin therapy, after treatment with SGLT2i, they showed a significant improvement in BW, fasting glucose and HbA1c despite a significant reduction in total insulin daily dose of 2.3 U (95% CI − 4.2 to − 0.4; p = 0.019).

Table 1

Changes in metabolic parameters and serum alanine aminotransferase levels before and after 6 months of treatment with dapagliflozin and empagliflozin

Patient parameters	Measurement time point		Mean change from baseline (95% CI)	p value
	Baseline	6 months post treatment initiation
BW (kg)	81.1 ± 18.1	79.5 ± 18.1	− 1.6 (− 2.2, − 1.1)	< 0.001*
BMI (Kg/m2)	30.5 ± 5.76	29.9 ± 5.75	− 0.6 (− 0.8, − 0.4)	< 0.001*
SBP (mmHg)	138 ± 19.0	132 ± 18.0	− 5.3 (− 9.1, − 1.5)	0.006*
DBP(mmHg)	77.4 ± 10.5	75.9 ± 11.2	− 1.5 (− 3.7, 0.6)	0.155
FPG (mmol/L)	9.12 ± 2.73	7.20 ± 1.91	− 1.9 (− 2.5, − 1.4)	< 0.001*
HbA1c (%)	8.56 ± 1.35	7.55 ± 0.90	− 1.0 (− 1.2, − 0.8)	< 0.001*
ALT (U/L)	40.3 ± 28.0	29.0 ± 14.1	− 11.3 (− 15.1, − 7.6)	< 0.001*
AST (U/L)	28.2 ± 13.2	23.1 ± 7.44	− 5.1 (− 7.1, − 3.1)	< 0.001*
Albumin (g/L)	43.3 ± 3.11	43.9 ± 3.05	0.6 (0.1, 1.1)	0.017*
TC (mmol/L)	3.95 ± 0.87	3.96 ± 0.85	0.01 (− 0.12, 0.13)	0.887
TG (mmol/L)	1.84 ± 1.43	1.68 ± 1.20	− 0.16 (− 0.34. 0.03)	0.098
HDL (mmol/L)	1.10 ± 0.29	1.14 ± 0.27	0.04 (0.003, 0.08)	0.033*
LDL (mmol/L)	2.10 ± 0.69	2.07 ± 0.67	− 0.03 (− 0.13, 0.07)	0.572
eGFR (ml/min/1.73 m2)	91.3 ± 19.6	89.7 ± 20.6	− 1.6 (− 2.9, − 0.3)	0.016*

95% CI 95% confidence interval, BW body weight, BMI body mass index, SBP systolic blood pressure, DBP diastolic blood pressure, FG fasting glucose, HbA1c glycated hemoglobin, ALT serum alanine aminotransferase, AST serum aspartate aminotransferase, TC total cholesterol, TG triglyceride, HDL high-density lipoprotein, LDL low-density lipoprotein, eGFR estimated glomerular filtration rate

*Significant difference (p < 0.05) between data at baseline and at 26 weeks after initiation of sodium–glucose co-transporter 2 inhibitor (SGLT2i) therapy

SGLT2i Ameliorated Hepatic Dysfunction Among Chinese Participants with T2DM

Participants treated with SGLT2i for 6 months also had a significant reduction in their mean serum ALT levels from 40.3 ± 28.0 to 29.0 ± 14.1 U/L (p < 0.001) (Table 1), and the results were consistent among various subgroups, whether with or without background insulin therapy, and whether dapagliflozin-treated or empagliflozin-treated (Fig. 1) The serum AST levels also decreased significantly from 28.2 ± 13.2 to 23.1 ± 7.5 U/L (p < 0.001), and the serum albumin increased significantly from 43.3 ± 3.1 to 43.9 ± 3.1 U/L (p = 0.017) after treatment with SGLT2i. Pearson’s correlation analysis revealed a positive correlation between the reduction in serum ALT levels after SGLT2i treatment and baseline BW (r = 0.231; p = 0.014), log-transformed ALT (r = 0.747; p < 0.001) and eGFR levels (r = 0.230; p = 0.014), suggesting that participants who had higher BW and serum ALT and eGFR levels at baseline experienced a significantly greater improvement in serum ALT levels after 6 months of treatment with SGLT2i. Moreover, the reduction in serum ALT levels after 6 months of SGLT2i treatment also correlated positively with log-transformed changes in AST (r = 0.465; p = < 0.001), fasting glucose (r = 0.248; p = 0.014) and HbA1c (r = 0.192; p = 0.043) but not with log-transformed changes in BW, SBP, serum albumin or eGFR levels (Table 2) The improvements in serum ALT levels after 6 months did not differ statistically between participants treated with dapagliflozin versus empagliflozin (p = 0.815), nor between participants with and without background insulin therapy (p = 0.429). Since the changes in AST and ALT levels were highly correlated (r = 0.465, p < 0.001), as were the changes in the fasting glucose and HbA1c (r = 0.448, p < 0.001), to avoid multi-collinearity, in the multiple linear regression analysis, either the change in fasting glucose or HbA1c was included in the model. Nonetheless, two separate analyses (Table 3) showed that the reduction in serum ALT levels was independently associated with the changes in both HbA1c and fasting glucose (p = 0.043 for change in HbA1c and p = 0.014 for change in fasting glucose), but not with the changes in other clinical variables, including BW. Using either the conventional serum ALT cutoff of 40 U/L [19, 20] or the cutoff of 30 U/L as suggested by some authors for Asians [20, 21], there was a significant increase in participants with normal ALT levels after 6 months of treatment with SGLT2i, compared with baseline (Fig. 2).

Fig. 1

Change in serum alanine aminotransferase levels from baseline to 6 months after initiation of treatment with dapagliflozin or empagliflozin. a All participants, b participants on dapagliflozin, c participants on empagliflozin, d participants on insulin, e participants not on insulin)

Table 2

Pearson’s correlation analysis showing the correlation of the reduction in serum alanine aminotransferase levels with baseline and changes in other variables at 6 months of treatment

Patients parameters	R	p value
Baseline variables
BW	0.231	0.014*
ALTa	0.614	< 0.001*
ASTa	0.401	< 0.001*
Albumina	0.088	0.381
HbA1c	0.007	0.938
FPG	− 0.026	0.795
SBP	0.095	0.325
eGFR (ml/min/1.73 m2)	0.216	0.023*
Change from baseline variablesa
BW	0.031	0.743
AST	0.465	< 0.001*
Albumin	0.083	0.411
HbA1c	0.192	0.043*
FPG	0.248	0.014*
SBP	0.003	0.978
eGFR (ml/min/1.73 m2)	− 0.150	0.122

*Significant difference at p < 0.05

aLog-transformed before analysis

Table 3

Multiple linear regression analysis showing the association between the reduction in serum alanine aminotransferase levels and change in glycated hemoglobin and fasting glucose levels, respectively, and other clinical variables after 6 months

Changes in clinical variablesa	Model 1			Model 2
	Standardized beta	Beta (95% CI)	p value	Standardized beta	Beta (95% CI)	p value
HbA1c in Model
Weight	0.007	0.067 (− 1.711, 1.845)	0.940	–	–	–
Albumin	0.122	0.713 (− 0.400, 1.826)	0.207	–	–	–
HbA1c	0.190	0.510 (0.001, 1.018)	0.049*	0.192	0.514 (0.017, 1.011)	0.043*
SBP	− 0.008	− 0.019 (− 0.481, 0.443)	0.936	–	–	–
eGFR	− 0.149	− 0.535 (− 1.219, 0.148)	0.124	–	–	–
Fasting glucose in Model
Weight	− 0.003	− 0.026 (− 1.793, 1.740)	0.976	–	–	–
Albumin	0.112	0.656 (− 0.443, 1.755)	0.239	–	–	–
FG	0.230	0.252 (0.048, 0.456)	0.016*	0.232	0.255 (0.053, 0.456)	0.014*
Systolic BP	0.021	0.052 (− 0.405, 0.508)	0.822	–	–	–
eGFR	− 0.155	− 0.557 (− 1.233, 0.119)	0.105	–	–	–

95% CI 95% confidence interval, HbA1c glycated hemoglobin, SBP systolic blood pressure, eGFR estimated glomerular filtration rate, FG fasting glucose

*Statistics test for comparision of ALT distribution between baseline and 6 months

aAll variables were log-transformed before analysis

Fig. 2

Participants at baseline and after 6 months of treatment with dapagliflozin or empagliflozin, stratified by their serum alanine aminotransferase levels (ALT). a ALT cutoff of 40 U/L, b ALT cutoff of 30 U/L; comparisons between baseline and 6 months ALT distributions by Fisher's exact test

Discussions

To our knowledge, our study is the first to explore the relationship between the amelioration of hepatic dysfunction and the improvement in various metabolic parameters brought about by dapagliflozin and empagliflozin among Chinese subjects with T2DM. Although SGLT2i exert a glucose-lowering effect together with weight loss, both of which are beneficial in the management of NAFLD, our findings demonstrated that dapagliflozin and empagliflozin reduced serum ALT levels as a class effect, at least in part through an improvement in glycemia, but not through BW reduction, as reflected by the significant correlation between ALT reduction and changes in fasting glucose and HbA1c levels, but not with the change in BW.

SGLT2i have been shown to reduce serum ALT levels in clinical trials, although the patients in these trials were mostly from Western populations. A recent meta-analysis of 43 randomized controlled trials reported that serum ALT levels fell by 0.21 IU/L (95% CI 0.33–0.10) after treatment with SGLT2i across all included studies [14]. However, the mechanism by which SGLT2i ameliorated hepatic dysfunction in T2DM remains controversial. Based on the results of their pooled analysis of data from four placebo-controlled and two active-controlled studies of canagliflozin, Leiter et al. attributed the significant improvement in serum ALT levels to combined HbA1c and body BW reduction, further noting that each 1% reduction in BW and HbA1c was associated with a 2 and 7% improvement in serum ALT levels, respectively [15]. Our findings are in line with their results with regard to the contribution of glycemic improvement towards the amelioration of hepatic dysfunction being much more important than BW reduction. A number of other animal and human studies using other SGLT2i also reported different findings from those observed in the pooled analysis of canagliflozin—although differences in sample size, study design and patient populations may explain (part of) the discrepancy in clinical outcomes. For example, in a mouse model of non-alcoholic steatohepatitis with diabetes, the use of empagliflozin decreased hepatic expression of several inflammatory genes, including tumor necrosis factor alpha, monocyte chemoattractant protein-1, interleukin-6 and suppressor of cytokine signaling-3, also independent of BW reduction [16]. In a recent randomized, active-controlled trial involving human participants which compared the effects of pioglitazone and ipragliflozin on NAFLD, among the 32 subjects on ipragliflozin, despite their significant improvement in glycemic control, BW and subcutaneous and visceral fat after 24 weeks, the reductions in serum ALT levels correlated only with changes in BW but not with changes in visceral fat or HbA1c level [22]. On the other hand, in that same study, improvement in the liver-to-spleen attenuation ratio, which is a reflection of hepatic steatosis, correlated with changes in BW and HbA1c and adiponectin levels [22]. However, in another Japanese study involving 48 Japanese subjects with T2DM, ipragliflozin was shown to reduce serum ALT levels irrespective of the reduction in BW, although the authors did not evaluate the relationship between glycemic improvement and the amelioration of hepatic dysfunction [17]. Taken together with the findings of the current study, these published data would suggest that the benefit of SGLT2i on hepatic dysfunction is likely to be a class effect, but the predominant effect through which SGLT2i ameliorate hepatic dysfunction in subjects with T2DM is still open to discussion. Theoretically, patients with NAFLD are required to lose up to 10% of their BW in order to improve necroinflammation, while a weight loss of 3–5% might only improve hepatic steatosis [23]. In our study, subjects experienced a mean weight reduction of 1.6 kg over 6 months, in keeping with the generally expected weight loss of 1.5–3.5 kg reported among SGLT2i users over a period of 6–12 months [8]; this was equivalent to around 2% of their baseline BW. Given this modest degree of weight loss brought by SGLT2i in our subjects, it may still be reasonable to suggest that the amelioration of hepatic dysfunction in our study population, as reflected by the improvement in liver enzyme levels, could be due to some other factors beyond weight reduction.

Our findings also highlight the role of glucotoxicity in modulating the progression of hepatic dysfunction. Chronic hyperglycemia can induce oxidative stress and endoplasmic reticulum stress responses and decrease AMP-activated protein kinase activity, hence promoting insulin resistance, hepatic steatosis and hepatocellular injury [24]. Although we found that the improvement in hepatic dysfunction significantly correlated with changes in glycemic parameters, the contribution of a direct effect of SGLT2i remains a possibility. In a randomized active-controlled trial of canagliflozin versus sitagliptin, despite a similar HbA1c improvement in both arms, there was a reduction in mean ALT levels among subjects randomized to canagliflozin 100 mg that was not observed among those randomized to sitagliptin [25]. Furthermore, dapagliflozin had recently been shown to increase circulating adiponectin and zinc-A2-glycoprotein levels in Chinese subjects with T2DM [26]. The authors in that study attributed their findings to peroxisome proliferator activated-receptor (PPAR) gamma activation. Nonetheless, the results of our study would support a role of hyperglycemia (and possibly secondary improvement in insulin resistance), in the amelrioation of hepatic dysfunction by SGLT2i, although this hypothesis warrants validation in further mechanistic studies.

Our study has several limitations. First, the sample size was relatively small. Second, results from histological or imaging studies were not available in the majority of our study subjects as confirmation of the cause of hepatic dysfunction. However, given the mild degree of hepatic dysfunction, it might be impractical to perform liver biopsy in all of our subjects. Furthermore, although ultrasound scan of the liver is a common tool to screen for NAFLD, this tool can only pick up hepatic steatosis of ≥ 30%. Last but not least, due to the clinical audit nature of the study, the causal relationship between the improvement in hepatic dysfunction and the changes in clinical variables could not be ascertained. Moreover, fasting insulin levels were not measured and hence conventional measures of the insulin resistance like homeostasis model assessment of insulin resistance (HOMA-IR) could not be assessed. Nonetheless, compared to most existing studies that have examined the relationship between SGLT2i and hepatic dysfunction, the majority of which comprised subjects with a relatively shorter duration of diabetes and not on insulin therapy, our study provides real-world observational data to support the use of SGLT2i in subjects with hepatic dysfunction across different patient populations of T2DM.

Conclusions

The use of SGLT2i is a novel approach to glycemic control and is accompanied by multiple demonstrable cardio-metabolic and renal benefits beyond its glucose-lowering effect. Our study results provide further support to the recent suggestion of considering SGLT2i in subjects comorbid with T2DM and NAFLD. With SGLT2i becoming available to such an enormous diabetic population in China, we have provided timely evidence that among Chinese subjects with T2DM, with or without background insulin therapy, dapagliflozin and empagliflozin not only improved metabolic parameters, but they also ameliorated hepatic dysfunction as a class, in part through alleviation of hyperglycemia, independent of BW reduction. Further prospective studies with larger sample sizes are certainly warranted to provide mechanistic insights into the benefits of SGLT2i in reversing hepatic dysfunction of T2DM.

Below is the link to the electronic supplementary material.

Supplementary material 1 (pdf 128 kb)