Literature DB >> 29322452

Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase Screening Assays.

Yan Li1, Bing Chen1, Shenglin Huang2.   

Abstract

Circular RNAs from back-spliced exons (circRNAs) represent a novel class of widespread and endogenous RNAs in eukaryotes. circRNAs may bind to microRNAs (miRNAs) and inhibit the activity of miRNAs. Alternatively, miRNAs could also directly target circRNAs and regulate the expression of circRNAs. Here we describe the Argonaute2 (AGO2) RNA immunoprecipitation (RIP) and luciferase screening assays to identify the interaction between circRNAs and miRNAs. The AGO2 RIP assay evaluates the potential of the interaction between circRNAs and miRNAs. The luciferase screening assay investigates the targeting miRNAs and the detail binding sites for a specific circRNA.

Entities:  

Keywords:  Circular RNA; Luciferase screening assay; RNA immunoprecipitation; microRNA

Mesh：

Substances：

Year:  2018        PMID： 29322452     DOI： 10.1007/978-1-4939-7562-4_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

Review 1.  CircRNAs: Insights into Gastric Cancer.

Authors:  Sanjana Rajgopal; Sherine Joanna Fredrick; Venkatachalam Deepa Parvathi
Journal:  Gastrointest Tumors       Date:  2021-07-16

2.  circFAM120B functions as a tumor suppressor in esophageal squamous cell carcinoma via the miR-661/PPM1L axis and the PKR/p38 MAPK/EMT pathway.

Authors:  Huan Song; Dan Tian; Jian Sun; Xuhua Mao; Weimin Kong; Dian Xu; Ye Ji; Beibei Qiu; Mengyao Zhan; Jianming Wang
Journal:  Cell Death Dis       Date:  2022-04-18       Impact factor: 9.685

Review 3.  Circular RNAs: a new frontier for cancer diagnosis and therapy.

Authors:  Miaoci Zhang; Yan Xin
Journal:  J Hematol Oncol       Date:  2018-02-13       Impact factor: 17.388

Review 4.  Advances in circular RNAs and their roles in breast Cancer.

Authors:  Xuehui Wang; Lin Fang
Journal:  J Exp Clin Cancer Res       Date:  2018-08-29

5.  CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer.

Authors:  Hongbin Li; Xiaodong Jin; Bingtao Liu; Pengcheng Zhang; Weiqiang Chen; Qiang Li
Journal:  BMC Cancer       Date:  2019-08-22       Impact factor: 4.430

Review 6.  Biogenesis, functions and clinical significance of circRNAs in gastric cancer.

Authors:  Chan Shan; Yinfeng Zhang; Xiaodan Hao; Jinning Gao; Xinzhe Chen; Kun Wang
Journal:  Mol Cancer       Date:  2019-09-13       Impact factor: 27.401

7.  circPARD3 drives malignant progression and chemoresistance of laryngeal squamous cell carcinoma by inhibiting autophagy through the PRKCI-Akt-mTOR pathway.

Authors:  Wei Gao; Huina Guo; Min Niu; Xiwang Zheng; Yuliang Zhang; Xuting Xue; Yunfeng Bo; Xiaoya Guan; Zhongxun Li; Yujia Guo; Long He; Yu Zhang; Li Li; Jimin Cao; Yongyan Wu
Journal:  Mol Cancer       Date:  2020-11-24       Impact factor: 27.401

8.  Genome-Wide Identification of CircRNAs of Infective Larvae and Adult Worms of Parasitic Nematode, Haemonchus contortus.

Authors:  Caixian Zhou; Yao Zhang; Simin Wu; Zhiheng Wang; Waresi Tuersong; Chunqun Wang; Feng Liu; Min Hu
Journal:  Front Cell Infect Microbiol       Date:  2021-11-22       Impact factor: 5.293

9.  Circular RNA circCORO1C promotes laryngeal squamous cell carcinoma progression by modulating the let-7c-5p/PBX3 axis.

Authors:  Yongyan Wu; Yuliang Zhang; Xiwang Zheng; Fengsheng Dai; Yan Lu; Li Dai; Min Niu; Huina Guo; Wenqi Li; Xuting Xue; Yunfeng Bo; Yujia Guo; Jiangbo Qin; Yixiao Qin; Hongliang Liu; Yu Zhang; Tao Yang; Li Li; Linshi Zhang; Rui Hou; Shuxin Wen; Changming An; Huizheng Li; Wei Xu; Wei Gao
Journal:  Mol Cancer       Date:  2020-06-02       Impact factor: 27.401

10.  A Novel Intronic Circular RNA Antagonizes Influenza Virus by Absorbing a microRNA That Degrades CREBBP and Accelerating IFN-β Production.

Authors:  Zhiyuan Qu; Fei Meng; Jianzhong Shi; Guohua Deng; Xianying Zeng; Jinying Ge; Yanbing Li; Liling Liu; Pucheng Chen; Yongping Jiang; Chengjun Li; Hualan Chen
Journal:  mBio       Date:  2021-07-20       Impact factor: 7.867
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