Literature DB >> 29322440

Characterization and Validation of Circular RNA and Their Host Gene mRNA Expression Using PCR.

Andreas W Heumüller1, Jes-Niels Boeckel2.   

Abstract

Polymerase chain reaction enables the detection and characterization of circular RNA expression. The use of divergent primer pairs flanking the back-splice site, being the unique sequence element of a circular RNA, enables the detection of circular RNA expression. Here we describe the basic techniques to detect different circular transcripts of a gene or one circular RNA specifically by PCR and highlight the advantages and drawbacks of both.

Keywords:  Back-splice site; CircRNA; Circular RNA; Divergent primer; Polymerase chain reaction

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Substances：

Year:  2018        PMID： 29322440     DOI： 10.1007/978-1-4939-7562-4_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  3 in total

1.  Detecting of chloroplast circular RNAs in Arabidopsis thaliana.

Authors:  Shuai Liu; Qiaojun Wang; Xinyu Li; Guibin Wang; Yinglang Wan
Journal:  Plant Signal Behav       Date:  2019-05-27

2.  Reduction of A-to-I RNA editing in the failing human heart regulates formation of circular RNAs.

Authors:  Karoline E Kokot; Jasmin M Kneuer; David John; Sabine Rebs; Maximilian N Möbius-Winkler; Stephan Erbe; Marion Müller; Michael Andritschke; Susanne Gaul; Bilal N Sheikh; Jan Haas; Holger Thiele; Oliver J Müller; Susanne Hille; Florian Leuschner; Stefanie Dimmeler; Katrin Streckfuss-Bömeke; Benjamin Meder; Ulrich Laufs; Jes-Niels Boeckel
Journal:  Basic Res Cardiol       Date:  2022-06-23       Impact factor: 12.416

3.  Circular RNAs are abundantly expressed and upregulated during repair of the damaged endometrium by Wharton's jelly-derived mesenchymal stem cells.

Authors:  Baolan Sun; Lei Shi; Qin Shi; Yao Jiang; Zhangyao Su; Xiaoqing Yang; Yuquan Zhang
Journal:  Stem Cell Res Ther       Date:  2018-11-15       Impact factor: 6.832
  3 in total

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