Literature DB >> 2932110

Identification of a cDNA clone in lambda gt11 for the transacylase component of branched chain ketoacid dehydrogenase.

S Litwer, D J Danner.   

Abstract

Two cDNA clones for the transacylase protein of the branched chain ketoacid dehydrogenase complex [E.C. 1.2.4.4] have been isolated from a human fetal liver cDNA expression library in lambda gt11 using antibody selection. By selective antibody elution from nitrocellulose filters containing the fusion proteins, it was determined that these inserts represent the transacylase protein. These data support the hypothesis that this protein is synthesized in the cytosol on transcripts independent of the other proteins of the branched chain ketoacid dehydrogenase complex.

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Year:  1985        PMID： 2932110     DOI： 10.1016/0006-291x(85)91333-6

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  4 in total

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Authors:  F Peinemann; D J Danner
Journal:  J Inherit Metab Dis       Date:  1994       Impact factor: 4.982

2.  Mitochondrial import and processing of an in vitro synthesized human prebranched chain acyltransferase fragment.

Authors:  S Litwer; D J Danner
Journal:  Am J Hum Genet       Date:  1988-11       Impact factor: 11.025

3.  Molecular cloning of genes encoding branched-chain keto acid dehydrogenase of Pseudomonas putida.

Authors:  P J Sykes; G Burns; J Menard; K Hatter; J R Sokatch
Journal:  J Bacteriol       Date:  1987-04       Impact factor: 3.490

4.  Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene.

Authors:  T Pihlajaniemi; T Helaakoski; K Tasanen; R Myllylä; M L Huhtala; J Koivu; K I Kivirikko
Journal:  EMBO J       Date:  1987-03       Impact factor: 11.598
  4 in total

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