D Apostolovic1, R Rodrigues2, P Thomas1, M Starkhammar3, C Hamsten1, M van Hage1,2. 1. Department of Medicine Solna, Immunology and Allergy Unit, Karolinska Institutet and University Hospital, Stockholm, Sweden. 2. Department of Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, Stockholm, Sweden. 3. Department of Internal Medicine, Södersjukhuset, Stockholm, Sweden.
Abstract
BACKGROUND: The galactose-α-1,3-galactose (α-Gal) epitope is involved in red meat allergy. As α-Gal is structurally similar to the blood group B-antigen, we explored the relationship between the immune responses to α-Gal- and the B-antigen in red meat-allergic patients compared to healthy A/O or B blood donors. METHODS: Sera from 51 red meat-allergic patients IgE-positive to α-Gal and 102 healthy blood donors (51 blood group A/O; 51 blood group B) were included. α-Gal- and B-antigen-specific IgE (ImmunoCAP) and IgG/IgG1-4 (ELISA) responses were determined. Basophil activation tests were performed. RESULTS: Fifteen healthy donors were IgE positive to α-Gal, of which 3 had blood group B. The allergic patients had significantly higher α-Gal IgE levels compared to the healthy donors. The majority of the allergic patients, but none of the healthy donors, had IgE against the B-antigen. Inhibition studies revealed cross-reactivity between α-Gal and the B-antigen. The biological activity of the B-antigen was confirmed by basophil activation tests. Anti-α-Gal IgG1 and IgG4 levels were significantly higher in the patients compared to the healthy donors. Moreover, the IgG response to the B-antigen was comparable between the allergic patients and healthy A/O donors. CONCLUSION: Red meat-allergic patients showed significantly higher α-Gal IgE, IgG1 , and IgG4 levels, reflecting a Th2 response, compared to healthy blood donors. Blood group B donors had significantly reduced antibody responses to α-Gal, due to similarities with the B-antigen, resulting in a lower risk of sensitization to α-Gal and development of red meat allergy.
BACKGROUND: The galactose-α-1,3-galactose (α-Gal) epitope is involved in red meat allergy. As α-Gal is structurally similar to the blood group B-antigen, we explored the relationship between the immune responses to α-Gal- and the B-antigen in red meat-allergicpatients compared to healthy A/O or B blood donors. METHODS: Sera from 51 red meat-allergicpatients IgE-positive to α-Gal and 102 healthy blood donors (51 blood group A/O; 51 blood group B) were included. α-Gal- and B-antigen-specific IgE (ImmunoCAP) and IgG/IgG1-4 (ELISA) responses were determined. Basophil activation tests were performed. RESULTS: Fifteen healthy donors were IgE positive to α-Gal, of which 3 had blood group B. The allergicpatients had significantly higher α-Gal IgE levels compared to the healthy donors. The majority of the allergicpatients, but none of the healthy donors, had IgE against the B-antigen. Inhibition studies revealed cross-reactivity between α-Gal and the B-antigen. The biological activity of the B-antigen was confirmed by basophil activation tests. Anti-α-Gal IgG1 and IgG4 levels were significantly higher in the patients compared to the healthy donors. Moreover, the IgG response to the B-antigen was comparable between the allergicpatients and healthy A/O donors. CONCLUSION: Red meat-allergicpatients showed significantly higher α-Gal IgE, IgG1 , and IgG4 levels, reflecting a Th2 response, compared to healthy blood donors. Blood group B donors had significantly reduced antibody responses to α-Gal, due to similarities with the B-antigen, resulting in a lower risk of sensitization to α-Gal and development of red meat allergy.
Authors: Michael Levin; Danijela Apostolovic; Tilo Biedermann; Scott P Commins; Onyinye I Iweala; Thomas A E Platts-Mills; Eleonora Savi; Marianne van Hage; Jeffrey M Wilson Journal: Ann Allergy Asthma Immunol Date: 2019-03-26 Impact factor: 6.347
Authors: Jeffrey M Wilson; Alexander J Schuyler; Lisa Workman; Monica Gupta; Hayley R James; Jonathon Posthumus; Emily C McGowan; Scott P Commins; Thomas A E Platts-Mills Journal: J Allergy Clin Immunol Pract Date: 2019-03-30
Authors: Thomas A E Platts-Mills; Scott P Commins; Tilo Biedermann; Marianne van Hage; Michael Levin; Lisa A Beck; Maria Diuk-Wasser; Uta Jappe; Danijela Apostolovic; Michael Minnicozzi; Marshall Plaut; Jeffrey M Wilson Journal: J Allergy Clin Immunol Date: 2020-02-10 Impact factor: 10.793
Authors: Jeffrey M Wilson; Behnam Keshavarz; Hayley R James; Maya K C Retterer; Alexander J Schuyler; Alice Knoedler; Lisa J Workman; Lucy Ng'ang'a; Martha E Chico; Eva Rönmark; Peter W Heymann; Matthew S Perzanowski; Thomas A E Platts-Mills; Philip J Cooper Journal: J Allergy Clin Immunol Date: 2021-02-01 Impact factor: 10.793
Authors: Alejandro Cabezas-Cruz; Adnan Hodžić; Patricia Román-Carrasco; Lourdes Mateos-Hernández; Georg Gerhard Duscher; Deepak Kumar Sinha; Wolfgang Hemmer; Ines Swoboda; Agustín Estrada-Peña; José de la Fuente Journal: Front Immunol Date: 2019-05-31 Impact factor: 7.561
Authors: Jeffrey M Wilson; Behnam Keshavarz; Maya Retterer; Lisa J Workman; Alexander J Schuyler; Emily C McGowan; Charles Lane; Alaaddin Kandeel; Jane Purser; Eva Rönmark; Joseph LaRussa; Scott P Commins; Tina Merritt; Thomas A E Platts-Mills Journal: J Allergy Clin Immunol Date: 2020-06-06 Impact factor: 10.793
Authors: Adnan Hodžić; Lourdes Mateos-Hernández; Michael Leschnik; Pilar Alberdi; Ryan O M Rego; Marinela Contreras; Margarita Villar; José de la Fuente; Alejandro Cabezas-Cruz; Georg Gerhard Duscher Journal: Vaccines (Basel) Date: 2019-09-15