BACKGROUND: Brassica crops together with cereals represent the basis of world supplies. Due to their importance, the production losses caused by Xanthomonas campestris pv. campestris (Xcc) infection represent a high economic impact. Understanding molecular and biochemical mechanisms of plants is essential to develop resistant crops with durable protection against diseases. In this regard, metabolomics has emerged as a valuable technology to provide an overview of the biological status of a plant exposed to a disease. This study investigated the dynamic changes in the metabolic profile of Brassica oleracea plants during an Xcc infection from leaves collected at five different days post infection using a mass spectrometry approach. RESULTS: Results showed that Xcc infection causes dynamic changes in the metabolome of B. oleracea. Moreover, induction/repression pattern of the metabolites implicated in the response follows a complex dynamics during infection progression, indicating a complex temporal response. Specific metabolic pathways such as alkaloids, coumarins or sphingolipids are postulated as promising key role candidates in the infection response. CONCLUSION: This work tries to decipher the changes produced on Brassica crops metabolome under Xcc infection and represents a step forward in the understanding of B. oleracea-Xcc interaction.
BACKGROUND:Brassica crops together with cereals represent the basis of world supplies. Due to their importance, the production losses caused by Xanthomonas campestris pv. campestris (Xcc) infection represent a high economic impact. Understanding molecular and biochemical mechanisms of plants is essential to develop resistant crops with durable protection against diseases. In this regard, metabolomics has emerged as a valuable technology to provide an overview of the biological status of a plant exposed to a disease. This study investigated the dynamic changes in the metabolic profile of Brassica oleracea plants during an Xcc infection from leaves collected at five different days post infection using a mass spectrometry approach. RESULTS: Results showed that Xcc infection causes dynamic changes in the metabolome of B. oleracea. Moreover, induction/repression pattern of the metabolites implicated in the response follows a complex dynamics during infection progression, indicating a complex temporal response. Specific metabolic pathways such as alkaloids, coumarins or sphingolipids are postulated as promising key role candidates in the infection response. CONCLUSION: This work tries to decipher the changes produced on Brassica crops metabolome under Xcc infection and represents a step forward in the understanding of B. oleracea-Xcc interaction.
Authors: Kristian Peters; Anja Worrich; Alexander Weinhold; Oliver Alka; Gerd Balcke; Claudia Birkemeyer; Helge Bruelheide; Onno W Calf; Sophie Dietz; Kai Dührkop; Emmanuel Gaquerel; Uwe Heinig; Marlen Kücklich; Mirka Macel; Caroline Müller; Yvonne Poeschl; Georg Pohnert; Christian Ristok; Victor Manuel Rodríguez; Christoph Ruttkies; Meredith Schuman; Rabea Schweiger; Nir Shahaf; Christoph Steinbeck; Maria Tortosa; Hendrik Treutler; Nico Ueberschaar; Pablo Velasco; Brigitte M Weiß; Anja Widdig; Steffen Neumann; Nicole M van Dam Journal: Int J Mol Sci Date: 2018-05-06 Impact factor: 5.923
Authors: Konstantinos Giannoukos; Stamatios Giannoukos; Christina Lagogianni; Dimitrios I Tsitsigiannis; Stephen Taylor Journal: Sci Rep Date: 2020-12-03 Impact factor: 4.379