Jinsong Hong1, Ting Wang2, Zhibin Chen3, Haobo Pan2, Xiaohua Pan3. 1. Department of Orthopedic and Traumatology, Guangzhou Orthopedic HospitalGuangzhou, China. 2. Center for Human Tissue and Organ Degeneration and Shenzhen Key Laboratory of Marine Biomedical Materials, Shenzhen Institutes of Advanced Technology, China Academy of ScienceShenzhen, China. 3. Department of Orthopedics and Traumatology, Bao'an Hospital Affiliated to Southern Medical University and Shenzhen Eighth People's HospitalShenzhen 518100, China.
Abstract
BACKGROUND: This study characterized a novel rabbit model of subchondral bone bruise and investigated the intervening effect of calcitonin. METHODS: Bone bruise was implemented via controlled free-fall counterpoise on the medial tibial subchondral bone of 5-month-old New Zealand rabbits, with 3, 2.5, or 2 Joules of energy. Subsequent subchondral bone bruise was characterized via magnetic resonance imaging, micro computed tomography, and histology. Calcitonin was administered for 3 weeks, and the changes in subchondral bone were characterized. RESULTS: The severity of subchondral bone bruise lesions correlated with the energy applied. The lesions involved trabecular separation and reduced trabecular number, with bone marrow edema and trabecular micro-fracture. With calcitonin treatment, subchondral bone marrow edema subsided and trabecular ultrastructure repaired. CONCLUSION: Free fall counterpoise is a promising method to establish a subchondral bone bruise model in rabbits. Calcitonin injection is a potential treatment for subchondral bone bruise lesions.
BACKGROUND: This study characterized a novel rabbit model of subchondral bone bruise and investigated the intervening effect of calcitonin. METHODS:Bone bruise was implemented via controlled free-fall counterpoise on the medial tibial subchondral bone of 5-month-old New Zealand rabbits, with 3, 2.5, or 2 Joules of energy. Subsequent subchondral bone bruise was characterized via magnetic resonance imaging, micro computed tomography, and histology. Calcitonin was administered for 3 weeks, and the changes in subchondral bone were characterized. RESULTS: The severity of subchondral bone bruise lesions correlated with the energy applied. The lesions involved trabecular separation and reduced trabecular number, with bone marrow edema and trabecular micro-fracture. With calcitonin treatment, subchondral bone marrow edema subsided and trabecular ultrastructure repaired. CONCLUSION: Free fall counterpoise is a promising method to establish a subchondral bone bruise model in rabbits. Calcitonin injection is a potential treatment for subchondral bone bruise lesions.
Entities:
Keywords:
Rabbits model; calcitonin; magnetic resonance imaging; subchondral bone bruise
Authors: Patrice W J Vincken; Bert P M Ter Braak; Arian R van Erkel; Emile G Coerkamp; Walter M C Mallens; Johan L Bloem Journal: Eur Radiol Date: 2005-04-20 Impact factor: 5.315