Literature DB >> 29311322

In situ detection of live-to-dead bacteria ratio after inactivation by means of synchronous fluorescence and PCA.

Runze Li1, Umang Goswami1, Maria King2, Jie Chen3, Thomas C Cesario4, Peter M Rentzepis5.   

Abstract

The determination of live and dead bacteria is of considerable significance for preventing health care-associated infection in hospitals, field clinics, and other areas. In this study, the viable (live) and nonviable (dead) bacteria in a sample were determined by means of their fluorescence spectra and principal component analysis (PCA). Data obtained in this study show that it is possible to identify bacteria strains and determine the live/dead ratio after UV light inactivation and antibiotic treatment, in situ, within minutes. In addition, synchronous fluorescence scans enable the identification of bacterial components such as tryptophan, tyrosine, and DNA. Compared with the time-consuming plating and culturing methods, this study renders a means for rapid detection and determination of live and dead bacteria.

Entities:  

Keywords:  PCA; bacteria inactivation; fluorescence spectroscopy

Mesh：

Year:  2018        PMID： 29311322      PMCID： PMC5789938          DOI： 10.1073/pnas.1716514115

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


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Authors:  Runze Li; Dinesh Dhankhar; Jie Chen; Thomas C Cesario; Peter M Rentzepis
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