| Literature DB >> 29308895 |
Qingxiang Guo1, Chenhua Yu1, Chao Zhang1, Yongtao Li1, Tianqi Wang1, Zhi Huang1, Xin Wang1, Wei Zhou1, Yao Li1, Zhongxiang Qin1, Cheng Wang1, Ruifang Gao1, Yongwei Nie1, Yakun Ma1, Yi Shi1, Jianyu Zheng2, Shengyong Yang3, Yan Fan1,4, Rong Xiang1,5.
Abstract
On the basis of novel pyrazino[2,3-c]quinolin-2(1H)-one scaffold, we designed and identified a highly selective, potent and oral mTOR inhibitor, 9m. Compound 9m showed low nanomolar activity against mTOR (IC50 = 7 nM) and greater selectivity over the related PIKK family kinases, which demonstrated only modest activity against 3 out of the 409 protein kinases. In vitro assays, compound 9m exhibited high potency against human breast and cervical cancer cells and induced tumor cell cycle arrest and autophagy. 9m inhibited cellular phosphorylation of mTORC1 (pS6 and p4E-BP1) and mTORC2 (pAKT (S473)) substrates. In T-47D xenograft mouse model, oral administration of compound 9m led to significant tumor regression without obvious toxicity. In addition, this compound showed good pharmacokinetics. Collectively, due to its high potency and selectivity, compound 9m could be used as a mTOR drug candidate.Entities:
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Year: 2018 PMID： 29308895 DOI： 10.1021/acs.jmedchem.7b01402
Source DB: PubMed Journal: J Med Chem ISSN： 0022-2623 Impact factor: 7.446