Uttam Kumar Maji1,2, Pradipta Jana3, Mitali Chatterjee2, Sanmay Karmakar4, Arup Saha4, Tamal Kanti Ghosh5,6. 1. Department of Pathology, IPGME&R, Kolkata, 700020, India. 2. Department of Pharmacology, UCM, IPGME&R, Kolkata, 700020, India. 3. Sinha Institute of Medical Science and Technology, Kolkata, 700084, India. 4. Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India. 5. Department of Pathology, IPGME&R, Kolkata, 700020, India. ghoshtamal@hotmail.com. 6. Department of Health and Family Welfare, Goverment of West Bengal, Kolkata, India. ghoshtamal@hotmail.com.
Abstract
INTRODUCTION: Hypertension is a metabolic disease which is caused by vasoconstriction and that results into elevated blood pressure. A chronic hypertensive condition affects and even damages to various systems in the body. Presence of renal cortexin (r-cortexin), an antihypertensive protein, which is released from the kidney cortex controls the blood pressure. The effect of r-cortexin was mediated through nitric oxide (NO), a universal vasodilating agent. AIM: In our study, acetyl salicylic acid (aspirin), a well-known activator of the endothelial nitric oxide synthase (eNOS) induced r-cortexin synthesis. METHODS: The hypertensive rat model was prepared by injecting deoxy corticosterone acetate (DOCA). Synthesis of r-cortexin was measured by the anti-r-cortexin antibody which was raised in adult white Wister albino rat model. NO level was determined by using methemoglobin method and later confirmed by chemiluminescence method. Change in blood pressure was determined indirectly by using NIBP monitoring system. RESULTS: Aspirin increased the r-cortexin expression from 64.36 ± 12.6 nM to 216.7 ± 21.31 nM in DOCA induced hypertensive rats. The mechanism was proved with the findings of increased level of NO from 0.4 to 1.9 µM. The DOCA induced blood pressure was also decreased from 139.39 ± 7.36 mm of Hg to 116.57 ± 6.89 mm of Hg in case of systolic blood pressure and in case of diastolic pressure from 110.41 ± 7 mm of Hg to 86.4 ± 2.76 mm of Hg that are quite approximate. CONCLUSION: So, from this study it has been found that aspirin induces the r-cortexin synthesis in kidney cortex through the activation of eNOS in DOCA induced hypertensive rats.
INTRODUCTION:Hypertension is a metabolic disease which is caused by vasoconstriction and that results into elevated blood pressure. A chronic hypertensive condition affects and even damages to various systems in the body. Presence of renal cortexin (r-cortexin), an antihypertensive protein, which is released from the kidney cortex controls the blood pressure. The effect of r-cortexin was mediated through nitric oxide (NO), a universal vasodilating agent. AIM: In our study, acetyl salicylic acid (aspirin), a well-known activator of the endothelial nitric oxide synthase (eNOS) induced r-cortexin synthesis. METHODS: The hypertensiverat model was prepared by injecting deoxy corticosterone acetate (DOCA). Synthesis of r-cortexin was measured by the anti-r-cortexin antibody which was raised in adult white Wister albino rat model. NO level was determined by using methemoglobin method and later confirmed by chemiluminescence method. Change in blood pressure was determined indirectly by using NIBP monitoring system. RESULTS:Aspirin increased the r-cortexin expression from 64.36 ± 12.6 nM to 216.7 ± 21.31 nM in DOCA induced hypertensiverats. The mechanism was proved with the findings of increased level of NO from 0.4 to 1.9 µM. The DOCA induced blood pressure was also decreased from 139.39 ± 7.36 mm of Hg to 116.57 ± 6.89 mm of Hg in case of systolic blood pressure and in case of diastolic pressure from 110.41 ± 7 mm of Hg to 86.4 ± 2.76 mm of Hg that are quite approximate. CONCLUSION: So, from this study it has been found that aspirin induces the r-cortexin synthesis in kidney cortex through the activation of eNOS in DOCA induced hypertensiverats.
Authors: Marina C Dos Santos Moreira; Izabella S de Jesus Pinto; Aline A Mourão; James O Fajemiroye; Eduardo Colombari; Ângela A da Silva Reis; André H Freiria-Oliveira; Marcos L Ferreira-Neto; Gustavo R Pedrino Journal: Front Physiol Date: 2015-08-25 Impact factor: 4.566
Authors: Dike B Ojji; Elena Libhaber; John J Atherton; Bolaji Abdullahi; Ada Nwankwo; Karen Sliwa Journal: Medicine (Baltimore) Date: 2015-09 Impact factor: 1.817
Authors: Monique C Suarez-Giron; Anabel Castro-Grattoni; Marta Torres; Ramon Farré; Ferran Barbé; Manuel Sánchez-de-la-Torre; David Gozal; Cesar Picado; Josep M Montserrat; Isaac Almendros Journal: Front Physiol Date: 2018-05-24 Impact factor: 4.566