Literature DB >> 2930554

Evidence for the formation of 26-hydroxycholesterol by cytochrome P-450 in pig kidney mitochondria.

H Postlind1, K Wikvall.   

Abstract

Pig kidney mitochondria were found to catalyze the formation of 26-hydroxycholesterol, an inhibitor of cholesterol biosynthesis. The cholesterol 26-hydroxylase was purified 600-fold. It was present in a mitochondrial enzyme fraction enriched in cytochrome P-450. The cytochrome P-450 fraction required NADPH, mitochondrial ferredoxin and ferredoxin reductase for 26-hydroxylase activity. The mitochondria and the purified 26-hydroxylase preparation also catalyzed 26-hydroxylation of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol, and intermediate in cholic acid biosynthesis, and of 25-hydroxyvitamin D3. The role of extra-hepatic formation of 26-hydroxycholesterol is discussed.

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Year:  1989        PMID： 2930554     DOI： 10.1016/0006-291x(89)92227-4

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  4 in total

Review 1.  Review of progress in sterol oxidations: 1987-1995.

Authors:  L L Smith
Journal:  Lipids       Date:  1996-05       Impact factor: 1.880

2.  27-Oxygenation of C27-sterols and 25-hydroxylation of vitamin D3 in kidney: cloning, structure and expression of pig kidney CYP27A.

Authors:  H Postlind; F Hosseinpour; M Norlin; K Wikvall
Journal:  Biochem J       Date:  2000-04-15       Impact factor: 3.857

3.  Characterization of mitochondrial cytochromes P-450 from pig kidney and liver catalysing 26-hydroxylation of 25-hydroxyvitamin D3 and C27 steroids.

Authors:  T Bergman; H Postlind
Journal:  Biochem J       Date:  1991-06-01       Impact factor: 3.857

4.  Atherosclerosis and sterol 27-hydroxylase: evidence for a role of this enzyme in elimination of cholesterol from human macrophages.

Authors:  I Björkhem; O Andersson; U Diczfalusy; B Sevastik; R J Xiu; C Duan; E Lund
Journal:  Proc Natl Acad Sci U S A       Date:  1994-08-30       Impact factor: 11.205
  4 in total

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