Literature DB >> 2930518

Human microsomal glutathione S-transferase. Its involvement in the conjugation of hexachlorobuta-1,3-diene with glutathione.

L I McLellan1, C R Wolf, J D Hayes.   

Abstract

A microsomal glutathione S-transferase (GST) was purified from human liver. This enzyme was shown to have characteristics similar to those of the rat microsomal GST described by Morgenstern & De Pierre [(1983) Eur. J. Biochem. 134, 591-597]. The specific activity of human microsomal GST towards 1-chloro-2,4-dinitrobenzene or cumene hydroperoxide can be stimulated by treating the enzyme with N-ethylmaleimide. This enhancement of activity is accompanied by increased sensitivity to inhibition by haematin and cholic acid. The subunit Mr values of the rat and human enzymes are similar (approx. 17,300), and the proteins are immunologically related. During purification, both human and rat microsomal GST enzymes are the only hepatic proteins obtained from Triton X-100-solubilized microsomal fractions that show activity towards the nephrotoxin hexachlorobuta-1,3-diene. The involvement of microsomal GST in toxification reactions is discussed.

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Year:  1989        PMID： 2930518      PMCID： PMC1138327          DOI： 10.1042/bj2580087

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  29 in total

1.  Statistical estimations in enzyme kinetics.

Authors:  G N WILKINSON
Journal:  Biochem J       Date:  1961-08       Impact factor: 3.857

2.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  A set of inhibitors for discrimination between the basic isozymes of glutathione transferase in rat liver.

Authors:  S Yalçin; H Jensson; B Mannervik
Journal:  Biochem Biophys Res Commun       Date:  1983-07-29       Impact factor: 3.575

5.  Assays for differentiation of glutathione S-transferases.

Authors:  W H Habig; W B Jakoby
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

6.  Evidence for the occurrence of selenium-independent glutathione peroxidase activity in rat liver microsomes.

Authors:  C C Reddy; C P Tu; J R Burgess; C Y Ho; R W Scholz; E J Massaro
Journal:  Biochem Biophys Res Commun       Date:  1981-08-14       Impact factor: 3.575

7.  Microsomal glutathione S-transferase. Purification, initial characterization and demonstration that it is not identical to the cytosolic glutathione S-transferases A, B and C.

Authors:  R Morgenstern; C Guthenberg; J W Depierre
Journal:  Eur J Biochem       Date:  1982-11

8.  The enzymatic reaction of chlorotrifluoroethylene with glutathione.

Authors:  D R Dohn; M W Anders
Journal:  Biochem Biophys Res Commun       Date:  1982-12-31       Impact factor: 3.575

9.  Characterization of rat-liver microsomal glutathione S-transferase activity.

Authors:  R Morgenstern; J Meijer; J W Depierre; L Ernster
Journal:  Eur J Biochem       Date:  1980-02

10.  Microsomal glutathione transferase. Purification in unactivated form and further characterization of the activation process, substrate specificity and amino acid composition.

Authors:  R Morgenstern; J W DePierre
Journal:  Eur J Biochem       Date:  1983-08-15
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  16 in total

1.  Characterization of a novel microsomal glutathione S-transferase produced by Aspergillus ochraceus TS.

Authors:  J Datta; T B Samanta
Journal:  Mol Cell Biochem       Date:  1992-12-02       Impact factor: 3.396

2.  Glutathione S-transferases in alcoholic liver disease.

Authors:  D J Harrison; L May; P C Hayes; M M Haque; J D Hayes
Journal:  Gut       Date:  1990-08       Impact factor: 23.059

3.  Glutathione S-transferase class Kappa: characterization by the cloning of rat mitochondrial GST and identification of a human homologue.

Authors:  S E Pemble; A F Wardle; J B Taylor
Journal:  Biochem J       Date:  1996-11-01       Impact factor: 3.857

4.  Evidence that glutathione S-transferases B1B1 and B2B2 are the products of separate genes and that their expression in human liver is subject to inter-individual variation. Molecular relationships between the B1 and B2 subunits and other Alpha class glutathione S-transferases.

Authors:  J D Hayes; L A Kerr; A D Cronshaw
Journal:  Biochem J       Date:  1989-12-01       Impact factor: 3.857

5.  Glutathione S-transferases in human liver cancer.

Authors:  P C Hayes; L May; J D Hayes; D J Harrison
Journal:  Gut       Date:  1991-12       Impact factor: 23.059

6.  Microsomal glutathione S-transferase A1-1 with glutathione peroxidase activity from sheep liver: molecular cloning, expression and characterization.

Authors:  K S Prabhu; P V Reddy; E Gumpricht; G R Hildenbrandt; R W Scholz; L M Sordillo; C C Reddy
Journal:  Biochem J       Date:  2001-12-01       Impact factor: 3.857

7.  Distribution of glutathione S-transferase isoenzymes in human kidney: basis for possible markers of renal injury.

Authors:  D J Harrison; R Kharbanda; D S Cunningham; L I McLellan; J D Hayes
Journal:  J Clin Pathol       Date:  1989-06       Impact factor: 3.411

8.  Lactococcus lactis is an Efficient Expression System for Mammalian Membrane Proteins Involved in Liver Detoxification, CYP3A4, and MGST1.

Authors:  Sana Bakari; Mehdi Lembrouk; Laura Sourd; Fares Ousalem; François André; Stéphane Orlowski; Marcel Delaforge; Annie Frelet-Barrand
Journal:  Mol Biotechnol       Date:  2016-04       Impact factor: 2.695

9.  Purification to homogeneity and the N-terminal sequence of human leukotriene C4 synthase: a homodimeric glutathione S-transferase composed of 18-kDa subunits.

Authors:  D W Nicholson; A Ali; J P Vaillancourt; J R Calaycay; R A Mumford; R J Zamboni; A W Ford-Hutchinson
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-01       Impact factor: 11.205

10.  Purification of human leukotriene C4 synthase.

Authors:  J F Penrose; L Gagnon; M Goppelt-Struebe; P Myers; B K Lam; R M Jack; K F Austen; R J Soberman
Journal:  Proc Natl Acad Sci U S A       Date:  1992-12-01       Impact factor: 11.205
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