Claudio Passariello1, Pierangelo Gigola2, Luca Testarelli3, Monica Puttini4, Serena Schippa1, Stefano Petti1. 1. Department of Public Health and Infectious Diseases, " Sapienza" University of Rome, Rome, Italy. 2. Department of Medical-Surgical Specialties, Radiologic and Public Health Sciences University of Brescia, Brescia, Italy. 3. Department of Odontostomatologic and Maxillo-Facial Sciences, "Sapienza" University of Rome, Rome, Italy. 4. Free Practice in Dentistry, Genova, Italy.
Abstract
AIMS: The present study aimed to investigate microbial patterns associated with disease progression and coinfection by different Herpesviruses in generalized aggressive periodontitis (GAP). METHODS: Microbiological samples were obtained from active (AS) and non-active (n-AS) sites in 165 subjects affected by GAP and were analyzed for 40 bacterial species by the Checkerboard DNA-DNA Hybridization technique and for Herpes simplex 1 (HSV-1), Human Cytomegalovirus (CMV), and Epstein Bar virus (EBV) by PCR.Common Factor Analysis and Multiple Regression Analysis were applied to disclose specific microbial patterns associated with the three viruses. RESULTS: Herpesviruses were detected in 37.6% of subjects. Detection of each of the searched viruses was associated with specific patterns of subgingival biofilm in AS. Logistic regression analyses evidenced several virus/bacteria associations: i) EBV with Aggregatibacter actinomycetemcomitans; ii) CMV with A. actinomycetemcomitans, Veillonella parvula, Parvimonas micra and Fusobacterium nucleatum subsp. polymorphum; iii) HSV-1 with Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium periodonticum and Staphylococcus aureus. CONCLUSIONS: Microbiological data suggest that Herpesviruses are probably not mere spectators of disease progression and that specific patterns of subgingival plaque are correlated with the presence of different Herpesviruses.
AIMS: The present study aimed to investigate microbial patterns associated with disease progression and coinfection by different Herpesviruses in generalized aggressive periodontitis (GAP). METHODS: Microbiological samples were obtained from active (AS) and non-active (n-AS) sites in 165 subjects affected by GAP and were analyzed for 40 bacterial species by the Checkerboard DNA-DNA Hybridization technique and for Herpes simplex 1 (HSV-1), Human Cytomegalovirus (CMV), and Epstein Bar virus (EBV) by PCR.Common Factor Analysis and Multiple Regression Analysis were applied to disclose specific microbial patterns associated with the three viruses. RESULTS: Herpesviruses were detected in 37.6% of subjects. Detection of each of the searched viruses was associated with specific patterns of subgingival biofilm in AS. Logistic regression analyses evidenced several virus/bacteria associations: i) EBV with Aggregatibacter actinomycetemcomitans; ii) CMV with A. actinomycetemcomitans, Veillonella parvula, Parvimonas micra and Fusobacterium nucleatum subsp. polymorphum; iii) HSV-1 with Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium periodonticum and Staphylococcus aureus. CONCLUSIONS: Microbiological data suggest that Herpesviruses are probably not mere spectators of disease progression and that specific patterns of subgingival plaque are correlated with the presence of different Herpesviruses.
Authors: Alice G Cheng; Hwan Keun Kim; Monica L Burts; Thomas Krausz; Olaf Schneewind; Dominique M Missiakas Journal: FASEB J Date: 2009-06-12 Impact factor: 5.191
Authors: Carlos J Rodriguez-Hernandez; Kevin J Sokoloski; Kendall S Stocke; Himabindu Dukka; Shunying Jin; Melissa A Metzler; Konstantin Zaitsev; Boris Shpak; Daonan Shen; Daniel P Miller; Maxim N Artyomov; Richard J Lamont; Juhi Bagaitkar Journal: Proc Natl Acad Sci U S A Date: 2021-12-21 Impact factor: 12.779
Authors: Freida Blostein; Sydney Foote; Elizabeth Salzman; Daniel W McNeil; Mary L Marazita; Emily T Martin; Betsy Foxman Journal: J Pediatric Infect Dis Soc Date: 2021-09-23 Impact factor: 5.235