Haruaki Kitagawa1, Saeki Miki-Oka2, Gen Mayanagi3, Yuki Abiko4, Nobuhiro Takahashi4, Satoshi Imazato5. 1. Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: h-kita@dent.osaka-u.ac.jp. 2. Department of Restorative Dentistry and Endodontology, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan. 3. Liaison Center for Innovative Dentistry, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. 4. Division of Oral Ecology and Biochemistry, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. 5. Department of Biomaterials Science, Osaka University Graduate School of Dentistry, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan.
Abstract
OBJECTIVES: Resin composites containing surface pre-reacted glass-ionomer (S-PRG) fillers have been reported to inhibit Streptococcus mutans growth on their surfaces, and their inhibitory effects were attributed to BO33- and F- ions. The aim of this study was to evaluate S. mutans acid production through glucose metabolism on resin composite containing S-PRG fillers and assess inhibitory effects of BO33- and F- on S. mutans metabolic activities. METHODS: The pH change through S. mutans acid production on experimental resin composite was periodically measured after the addition of glucose. Inhibitory effects of BO33- or F- solutions on S. mutans metabolism were evaluated by XTT assays and measurement of the acid production rate. RESULTS: The pH of experimental resin containing S-PRG fillers was significantly higher than that of control resin containing silica fillers (p < 0.05). OD450 values by XTT assays and S. mutans acid production rates significantly decreased in the presence of BO33- and F- compared with the absence of these ions (p < 0.05). CONCLUSIONS: pH reduction by S. mutans acid production was inhibited on resin composite containing S-PRG fillers. Moreover, S. mutans glucose metabolism and acid production were inhibited in the presence of low concentrations of BO33- or F-. CLINICAL SIGNIFICANCE: BO33- or F- released from resin composite containing S-PRG fillers exhibits inhibitory effects on S. mutans metabolism at concentrations lower than those which inhibit bacterial growth.
OBJECTIVES: Resin composites containing surface pre-reacted glass-ionomer (S-PRG) fillers have been reported to inhibit Streptococcus mutans growth on their surfaces, and their inhibitory effects were attributed to BO33- and F- ions. The aim of this study was to evaluate S. mutans acid production through glucose metabolism on resin composite containing S-PRG fillers and assess inhibitory effects of BO33- and F- on S. mutans metabolic activities. METHODS: The pH change through S. mutans acid production on experimental resin composite was periodically measured after the addition of glucose. Inhibitory effects of BO33- or F- solutions on S. mutans metabolism were evaluated by XTT assays and measurement of the acid production rate. RESULTS: The pH of experimental resin containing S-PRG fillers was significantly higher than that of control resin containing silica fillers (p < 0.05). OD450 values by XTT assays and S. mutans acid production rates significantly decreased in the presence of BO33- and F- compared with the absence of these ions (p < 0.05). CONCLUSIONS: pH reduction by S. mutans acid production was inhibited on resin composite containing S-PRG fillers. Moreover, S. mutansglucose metabolism and acid production were inhibited in the presence of low concentrations of BO33- or F-. CLINICAL SIGNIFICANCE: BO33- or F- released from resin composite containing S-PRG fillers exhibits inhibitory effects on S. mutans metabolism at concentrations lower than those which inhibit bacterial growth.
Authors: Ning Zhang; Ke Zhang; Xianju Xie; Zixiang Dai; Zeqing Zhao; Satoshi Imazato; Yousif A Al-Dulaijan; Faisal D Al-Qarni; Michael D Weir; Mark A Reynolds; Yuxing Bai; Lin Wang; Hockin H K Xu Journal: Nanomaterials (Basel) Date: 2018-06-01 Impact factor: 5.076
Authors: Ke Zhang; Bashayer Baras; Christopher D Lynch; Michael D Weir; Mary Anne S Melo; Yuncong Li; Mark A Reynolds; Yuxing Bai; Lin Wang; Suping Wang; Hockin H K Xu Journal: Materials (Basel) Date: 2018-09-17 Impact factor: 3.623