Literature DB >> 29292440

Direct detection of lysine side chain NH3+ in protein-heparin complexes using NMR spectroscopy.

Krishna Mohan Sepuru1, Junji Iwahara1, Krishna Rajarathnam1.   

Abstract

Two NMR observables, the NζH3+ peak in the HISQC spectrum and Nζ chemical shift difference between the free and heparin-bound forms, can identify binding-interface lysines in protein-heparin complexes. Unlike backbone chemical shifts, these direct probes are stringent and are less prone to either false positives or false negatives.

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Year:  2018        PMID： 29292440      PMCID： PMC5788726          DOI： 10.1039/c7an01406f

Source DB:  PubMed          Journal:  Analyst        ISSN： 0003-2654            Impact factor:   4.616


  20 in total

Review 1.  Using chemical shift perturbation to characterise ligand binding.

Authors:  Mike P Williamson
Journal:  Prog Nucl Magn Reson Spectrosc       Date:  2013-03-21       Impact factor: 9.795

2.  Molecular Basis of Chemokine CXCL5-Glycosaminoglycan Interactions.

Authors:  Krishna Mohan Sepuru; Balaji Nagarajan; Umesh R Desai; Krishna Rajarathnam
Journal:  J Biol Chem       Date:  2016-07-28       Impact factor: 5.157

3.  CXCL1/MGSA Is a Novel Glycosaminoglycan (GAG)-binding Chemokine: STRUCTURAL EVIDENCE FOR TWO DISTINCT NON-OVERLAPPING BINDING DOMAINS.

Authors:  Krishna Mohan Sepuru; Krishna Rajarathnam
Journal:  J Biol Chem       Date:  2015-12-31       Impact factor: 5.157

4.  NMR characterization of the electrostatic interaction of the basic residues in HDGF and FGF2 during heparin binding.

Authors:  Liang-Yuan Chiu; Kuo-Wei Hung; Siu-Cin Tjong; Yun-Wei Chiang; Shih-Che Sue
Journal:  Biochim Biophys Acta       Date:  2014-08-10

5.  Unambiguous determination of the ionization state of a glycoside hydrolase active site lysine by 1H-15N heteronuclear correlation spectroscopy.

Authors:  David K Y Poon; Mario Schubert; Jason Au; Mark Okon; Stephen G Withers; Lawrence P McIntosh
Journal:  J Am Chem Soc       Date:  2006-12-06       Impact factor: 15.419

Review 6.  Heparan sulfate and heparin interactions with proteins.

Authors:  Maria C Z Meneghetti; Ashley J Hughes; Timothy R Rudd; Helena B Nader; Andrew K Powell; Edwin A Yates; Marcelo A Lima
Journal:  J R Soc Interface       Date:  2015-09-06       Impact factor: 4.118

7.  Residue-specific pKa determination of lysine and arginine side chains by indirect 15N and 13C NMR spectroscopy: application to apo calmodulin.

Authors:  Ingemar André; Sara Linse; Frans A A Mulder
Journal:  J Am Chem Soc       Date:  2007-11-29       Impact factor: 15.419

8.  Solution NMR characterization of chemokine CXCL8/IL-8 monomer and dimer binding to glycosaminoglycans: structural plasticity mediates differential binding interactions.

Authors:  Prem Raj B Joseph; Philip D Mosier; Umesh R Desai; Krishna Rajarathnam
Journal:  Biochem J       Date:  2015-09-14       Impact factor: 3.857

9.  Solution structure of CXCL5--a novel chemokine and adipokine implicated in inflammation and obesity.

Authors:  Krishna Mohan Sepuru; Krishna Mohan Poluri; Krishna Rajarathnam
Journal:  PLoS One       Date:  2014-04-02       Impact factor: 3.240

10.  Chemokine CXCL1 mediated neutrophil recruitment: Role of glycosaminoglycan interactions.

Authors:  Kirti V Sawant; Krishna Mohan Poluri; Amit K Dutta; Krishna Mohan Sepuru; Anna Troshkina; Roberto P Garofalo; Krishna Rajarathnam
Journal:  Sci Rep       Date:  2016-09-14       Impact factor: 4.379
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  6 in total

Review 1.  NMR Methods for Characterizing the Basic Side Chains of Proteins: Electrostatic Interactions, Hydrogen Bonds, and Conformational Dynamics.

Authors:  Dan Nguyen; Chuanying Chen; B Montgomery Pettitt; Junji Iwahara
Journal:  Methods Enzymol       Date:  2018-09-27       Impact factor: 1.600

2.  Structural basis of chemokine interactions with heparan sulfate, chondroitin sulfate, and dermatan sulfate.

Authors:  Krishna Mohan Sepuru; Krishna Rajarathnam
Journal:  J Biol Chem       Date:  2019-08-27       Impact factor: 5.157

3.  Nucleotide Binding Modes in a Motor Protein Revealed by 31 P- and 1 H-Detected MAS Solid-State NMR Spectroscopy.

Authors:  Thomas Wiegand; Maarten Schledorn; Alexander A Malär; Riccardo Cadalbert; Alexander Däpp; Laurent Terradot; Beat H Meier; Anja Böckmann
Journal:  Chembiochem       Date:  2019-09-30       Impact factor: 3.164

4.  Neutrophil recruitment by chemokines Cxcl1/KC and Cxcl2/MIP2: Role of Cxcr2 activation and glycosaminoglycan interactions.

Authors:  Kirti V Sawant; Krishna Mohan Sepuru; Emily Lowry; Brigith Penaranda; Charles W Frevert; Roberto P Garofalo; Krishna Rajarathnam
Journal:  J Leukoc Biol       Date:  2020-09-02       Impact factor: 4.962

Review 5.  Glycosaminoglycan-Protein Interactions by Nuclear Magnetic Resonance (NMR) Spectroscopy.

Authors:  Vitor H Pomin; Xu Wang
Journal:  Molecules       Date:  2018-09-11       Impact factor: 4.411

Review 6.  Structural Insights Into How Proteoglycans Determine Chemokine-CXCR1/CXCR2 Interactions: Progress and Challenges.

Authors:  Krishna Rajarathnam; Umesh R Desai
Journal:  Front Immunol       Date:  2020-04-24       Impact factor: 7.561
  6 in total

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