Masahiro Hirasawa1, Chiaki Tsutsumi-Arai2, Kensuke Takakusaki3, Toyohisa Oya4, Kenji Fueki5, Noriyuki Wakabayashi6. 1. Department of Removable Partial Prosthodontics, Graduate School, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan. Electronic address: sawarpro@tmd.ac.jp. 2. Department of Oral Medicine and Stomatology, Tsurumi University School of Dental Medicine, 2-1-3, Tsurumi, Tsurumi-ku, Yokohama, Kanagawa, 230-8501, Japan. Electronic address: tsutsumi-c@tsurumi-u.ac.jp. 3. Department of Removable Partial Prosthodontics, Graduate School, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan. Electronic address: k.takakusaki.rpro@tmd.ac.jp. 4. Synthetic Organic Chemistry Laboratories, Research & Development Management Headquarters, FUJIFILM Corporation, 210, Nakanuma, Minamiashigara-shi, Kanagawa, 250-0193, Japan. Electronic address: toyohisa.oya@fujifilm.com. 5. Department of Removable Partial Prosthodontics, Graduate School, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan. Electronic address: kunfu.rpro@tmd.ac.jp. 6. Department of Removable Partial Prosthodontics, Graduate School, Tokyo Medical and Dental University (TMDU), 1-5-45, Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan. Electronic address: wakabayashi.rpro@tmd.ac.jp.
Abstract
OBJECTIVE: In this study, we aimed to investigate denture-base-resin coatings prepared with a crosslinkable co-polymer containing sulfobetaine methacrylamide (SBMAm) and the relationship between their surface characteristics and the initial adhesion of Candida albicans (C. albicans). METHODS: Acrylic resin discs were coated with co-polymers containing various concentrations of SBMAm and N,N'-(4,7,10-trioxa-1,13-tridecadiamine) diacrylamide (JDA) as crosslinking agent. Uncoated discs were used as controls. An acquired pellicle was formed on each disc using artificial saliva, and the discs were immersed in a suspension of C. albicans (JCM2085) cells. After incubation, tetrazolium salt (XTT-reduction) and colony forming unit (CFU) assays were performed and the morphogenesis of C. albicans was examined using scanning electron microscopy (SEM). The surface roughness, film thickness, and the water contact angle of each disc surface were measured. RESULTS: All coating groups showed significantly lower amounts of adhered C. albicans in the XTT-reduction and CFU assays than the control, confirmed by the SEM images. Many wrinkle structures were observed on the surfaces coated with co-polymers containing more than 30% SBMAm. There were no significant differences in surface roughness among all groups. The co-polymer films on the coated discs were less than 5.0 μm in thickness, and these surfaces exhibited significantly lower mean water contact angles than the control. CONCLUSION: Crosslinkable co-polymers containing SBMAm can enhance the hydrophilicity of the surface of denture-base resins and reduce the initial adhesion of C. albicans.
OBJECTIVE: In this study, we aimed to investigate denture-base-resin coatings prepared with a crosslinkable co-polymer containing sulfobetaine methacrylamide (SBMAm) and the relationship between their surface characteristics and the initial adhesion of Candida albicans (C. albicans). METHODS:Acrylic resin discs were coated with co-polymers containing various concentrations of SBMAm and N,N'-(4,7,10-trioxa-1,13-tridecadiamine) diacrylamide (JDA) as crosslinking agent. Uncoated discs were used as controls. An acquired pellicle was formed on each disc using artificial saliva, and the discs were immersed in a suspension of C. albicans (JCM2085) cells. After incubation, tetrazolium salt (XTT-reduction) and colony forming unit (CFU) assays were performed and the morphogenesis of C. albicans was examined using scanning electron microscopy (SEM). The surface roughness, film thickness, and the water contact angle of each disc surface were measured. RESULTS: All coating groups showed significantly lower amounts of adhered C. albicans in the XTT-reduction and CFU assays than the control, confirmed by the SEM images. Many wrinkle structures were observed on the surfaces coated with co-polymers containing more than 30% SBMAm. There were no significant differences in surface roughness among all groups. The co-polymer films on the coated discs were less than 5.0 μm in thickness, and these surfaces exhibited significantly lower mean water contact angles than the control. CONCLUSION: Crosslinkable co-polymers containing SBMAm can enhance the hydrophilicity of the surface of denture-base resins and reduce the initial adhesion of C. albicans.
Authors: Mohammed M Gad; Hala A Bahgat; Mohamed F Edrees; Abdulkareem Alhumaidan; Soban Qadir Khan; Neveen M Ayad Journal: J Int Soc Prev Community Dent Date: 2022-01-29
Authors: David Gräfe; Andreas Wickberg; Markus Michael Zieger; Martin Wegener; Eva Blasco; Christopher Barner-Kowollik Journal: Nat Commun Date: 2018-07-17 Impact factor: 14.919