Matthias Noll1, Sylvia Kleta2, Sascha Al Dahouk3. 1. University of Applied Sciences and Arts, Institute for Bioanalysis, Friedrich-Streib-Str. 2, D-96450 Coburg, Germany; German Federal Institute for Risk Assessment, National Reference Laboratory for Listeria monocytogenes, Diedersdorfer Weg 1, D-12277 Berlin, Germany. Electronic address: matthias.noll@hs-coburg.de. 2. German Federal Institute for Risk Assessment, National Reference Laboratory for Listeria monocytogenes, Diedersdorfer Weg 1, D-12277 Berlin, Germany. Electronic address: Sylvia.Kleta@bfr.bund.de. 3. German Federal Institute for Risk Assessment, National Reference Laboratory for Listeria monocytogenes, Diedersdorfer Weg 1, D-12277 Berlin, Germany; RWTH Aachen University, Department of Internal Medicine III, Pauwelsstraße 30, D-52074 Aachen, Germany. Electronic address: Sascha.Al-Dahouk@gmx.de.
Abstract
BACKGROUND: The objective of this study was to evaluate the susceptibility of 259 Listeria monocytogenes strains isolated from food and food-processing environments and patient samples in Germany to 14 antibiotics widely used in veterinary and human medicine. L. monocytogenes strains were isolated mainly from milk and milk products and classified according to their molecular serotypes IIa (n=112), IIb (n=41), IIc (n=36), IVa (n=1), IVb (n=66), and IVb-v1 (n=3). METHODS: Susceptibility tests were performed by using the automated 96-well based microdilution system Micronaut-S. Ampicillin, benzylpenicillin, ceftriaxone, ciprofloxacin, daptomycin, erythromcyin, gentamicin, linezolid, meropenem, rifampicin, tetracycline, tigecycline, trimethoprim/sulfamethoxazole and vancomycin were tested in at least five different concentrations. RESULTS: Among the 259 strains under study, 145 strains revealed multidrug-resistance (resistance to ≥3 antibiotics) and predominantly belonged to serotype IV (59%). Strains were mainly resistant to daptomycin, tigecycline, tetracycline, ciprofloxacin, ceftriaxone, trimethropim/sulfamethoxazole and gentamicin. CONCLUSIONS: Antibiotic resistance in general and multidrug-resistance in particular were more prevalent in L. monocytogenes strains isolated in Germany compared to similar reference stocks from other European countries and the USA but similar to stocks from China.
BACKGROUND: The objective of this study was to evaluate the susceptibility of 259 Listeria monocytogenes strains isolated from food and food-processing environments and patient samples in Germany to 14 antibiotics widely used in veterinary and human medicine. L. monocytogenes strains were isolated mainly from milk and milk products and classified according to their molecular serotypes IIa (n=112), IIb (n=41), IIc (n=36), IVa (n=1), IVb (n=66), and IVb-v1 (n=3). METHODS: Susceptibility tests were performed by using the automated 96-well based microdilution system Micronaut-S. Ampicillin, benzylpenicillin, ceftriaxone, ciprofloxacin, daptomycin, erythromcyin, gentamicin, linezolid, meropenem, rifampicin, tetracycline, tigecycline, trimethoprim/sulfamethoxazole and vancomycin were tested in at least five different concentrations. RESULTS: Among the 259 strains under study, 145 strains revealed multidrug-resistance (resistance to ≥3 antibiotics) and predominantly belonged to serotype IV (59%). Strains were mainly resistant to daptomycin, tigecycline, tetracycline, ciprofloxacin, ceftriaxone, trimethropim/sulfamethoxazole and gentamicin. CONCLUSIONS: Antibiotic resistance in general and multidrug-resistance in particular were more prevalent in L. monocytogenes strains isolated in Germany compared to similar reference stocks from other European countries and the USA but similar to stocks from China.
Authors: Mélodie Duval; Daniel Dar; Filipe Carvalho; Eduardo P C Rocha; Rotem Sorek; Pascale Cossart Journal: Proc Natl Acad Sci U S A Date: 2018-12-13 Impact factor: 11.205
Authors: Konstantinos Koutsoumanis; Ana Allende; Avelino Álvarez-Ordóñez; Declan Bolton; Sara Bover-Cid; Marianne Chemaly; Robert Davies; Alessandra De Cesare; Lieve Herman; Friederike Hilbert; Roland Lindqvist; Maarten Nauta; Giuseppe Ru; Marion Simmons; Panagiotis Skandamis; Elisabetta Suffredini; Héctor Argüello; Thomas Berendonk; Lina Maria Cavaco; William Gaze; Heike Schmitt; Ed Topp; Beatriz Guerra; Ernesto Liébana; Pietro Stella; Luisa Peixe Journal: EFSA J Date: 2021-06-17