Paraquat treatment modulates integrin associated protein (CD47) and basigin (CD147) expression and mitochondrial potential on erythroid cells in mice.

The present study is focused on the interaction of paraquat with the erythroid system in bone marrow and spleen of mice. Administration of paraquat (10 mg/kg of body weight i.p. on alternate days in C57Bl/6 mice) induced the level of reactive oxygen species in bone marrow (BM) on 7, 14, and 21 day time points but it was unchanged in spleen erythroid cell. A marked induction of CD147 expression in BM and spleen erythroid cells was observed in the paraquat treated mice. Paraquat treatment also modulated the CD47 expression in erythroid cells and its expression level was significantly higher on day 14, 21 and 28 in bone marrow and on day 14 and 21 in spleen. The expression level of mitochondrial potential and antioxidant genes SOD1, SOD2, GPX1 and FOXO3 expression was significantly reduced in BM erythroid cells but a reverse response was seen in spleen. Taken together, this study demonstrates that paraquat treatment modulates ROS production, mitochondrial membrane potential, and oxidative stress markers gene expression in the erythroid systems of C57Bl/6 mice.