Jing Yu1, Yifan Lv1, Wenjuan Di1, Juan Liu1, Xiaocen Kong1, Yunlu Sheng1, Min Huang1, Shan Lv1, Hanmei Qi1, Mei Gao2, Hui Liang3, Sarah Kim4, Zan Fu3, Hong Zhou4, Guoxian Ding1. 1. Department of Geratology, the First Affiliated Hospital to Nanjing Medical University, Nanjing, China. 2. Department of Anesthesia, the First Affiliated Hospital to Nanjing Medical University, Nanjing, China. 3. Department of General Surgery, the First Affiliated Hospital to Nanjing Medical University, Nanjing, China. 4. Bone Research Program, ANZAC Research Institute, University of Sydney, Sydney, New South Wales, Australia.
Abstract
OBJECTIVE: Given the rising prevalence of central obesity and the discovery that beige cells appear within white adipose tissue, strategies to enhance these energy-expending adipocytes or "browning" within white adipose depots have become of therapeutic interest to combat obesity and its associated disorders. This study focused on, the role of microRNA (miRNA)-27b-3p in human visceral adipose tissue (VAT) browning. METHODS: Expression of miR-27b-3p and UCP1 in VAT and serum of humans was measured. MiR-27b-3p was overexpressed or suppressed in human visceral stromal fraction cells to analyze the potential role of miR-27b-3p. RESULTS: UCP1 expression in human VAT decreased with elevated BMI and waist-hip ratio, whereas expression of miR-27b-3p was found to correlate positively with BMI and waist-hip ratio. High expression of miR-27b-3p was associated with reduced browning ability of human visceral adipocytes. Antagonism of miR-27b-3p led to the enhancement of browning ability in human visceral adipocytes. CONCLUSIONS: These findings highlight the decreased browning ability of VAT from humans with obesity and the role of miR-27b-3p in regulating browning of human visceral adipocytes. They suggest that miR-27b-3p should be further explored as a potential target for the treatment of central obesity.
OBJECTIVE: Given the rising prevalence of central obesity and the discovery that beige cells appear within white adipose tissue, strategies to enhance these energy-expending adipocytes or "browning" within white adipose depots have become of therapeutic interest to combat obesity and its associated disorders. This study focused on, the role of microRNA (miRNA)-27b-3p in human visceral adipose tissue (VAT) browning. METHODS: Expression of miR-27b-3p and UCP1 in VAT and serum of humans was measured. MiR-27b-3p was overexpressed or suppressed in human visceral stromal fraction cells to analyze the potential role of miR-27b-3p. RESULTS:UCP1 expression in human VAT decreased with elevated BMI and waist-hip ratio, whereas expression of miR-27b-3p was found to correlate positively with BMI and waist-hip ratio. High expression of miR-27b-3p was associated with reduced browning ability of human visceral adipocytes. Antagonism of miR-27b-3p led to the enhancement of browning ability in human visceral adipocytes. CONCLUSIONS: These findings highlight the decreased browning ability of VAT from humans with obesity and the role of miR-27b-3p in regulating browning of human visceral adipocytes. They suggest that miR-27b-3p should be further explored as a potential target for the treatment of central obesity.
Authors: Marcos Garcia-Lacarte; Maria L Mansego; M Angeles Zulet; J Alfredo Martinez; Fermin I Milagro Journal: Cells Date: 2019-11-30 Impact factor: 6.600
Authors: Kaiping Burrows; Leandra K Figueroa-Hall; Rayus Kuplicki; Jennifer L Stewart; Ahlam M Alarbi; Rajagopal Ramesh; Jonathan B Savitz; T Kent Teague; Victoria B Risbrough; Martin P Paulus Journal: Sci Rep Date: 2022-01-17 Impact factor: 4.379
Authors: Guoqi Li; Yihui Shao; Hong Chang Guo; Ying Zhi; Bokang Qiao; Ke Ma; Jie Du; Yong Qiang Lai; Yulin Li Journal: Cardiovasc Res Date: 2022-07-20 Impact factor: 13.081