Yosuke Shimamura1, Issei Takeuchi1, Hiroshi Terada1,2,3, Kimiko Makino4,2. 1. Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan. 2. Tokyo University of Science Center for Drug Delivery Research, Tokyo University of Science, Chiba, Japan. 3. Niigata University of Pharmacy and Applied Life Sciences, Niigata, Japan. 4. Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba, Japan makino@rs.noda.tus.ac.jp.
Abstract
BACKGROUND: Oral mucositis (OM), one of the side-effects induced by chemotherapy, has 40% incidence and the incidence rate increases to approximately 100% in combination with radiotherapy. We describe OM in ICR mice induced using 5-fluorouracil (5-FU) and 20% acetic acid. MATERIALS AND METHODS: We optimized the dose of 5-FU and 20% acetic acid and validated the efficacy of standard therapies for OM. RESULTS: All mice developed OM after administration of 5-FU and 20% acetic acid. Application of Kenalog® reduced maximum ulcer area and the duration of spontaneous recovery in a dose-dependent manner. CONCLUSION: We succeeded in developing a mouse model of OM induced by cancer chemotherapy. New drugs for OM induced by anticancer drugs can be evaluated simply by monitoring the WBC count in this mouse model. This model is expected to contribute to development of new drugs and elucidation of the mechanisms of ameliorating stomatitis as a side-effect of anticancer drugs. Copyright
BACKGROUND:Oral mucositis (OM), one of the side-effects induced by chemotherapy, has 40% incidence and the incidence rate increases to approximately 100% in combination with radiotherapy. We describe OM in ICR mice induced using 5-fluorouracil (5-FU) and 20% acetic acid. MATERIALS AND METHODS: We optimized the dose of 5-FU and 20% acetic acid and validated the efficacy of standard therapies for OM. RESULTS: All mice developed OM after administration of 5-FU and 20% acetic acid. Application of Kenalog® reduced maximum ulcer area and the duration of spontaneous recovery in a dose-dependent manner. CONCLUSION: We succeeded in developing a mouse model of OM induced by cancer chemotherapy. New drugs for OM induced by anticancer drugs can be evaluated simply by monitoring the WBC count in this mouse model. This model is expected to contribute to development of new drugs and elucidation of the mechanisms of ameliorating stomatitis as a side-effect of anticancer drugs. Copyright
Authors: Sohi Kang; Eun Joo Jang; Hyun Min Jo; Seong Soo Kang; Mu Seong Lee; Sei Young Yun; Kyung Mi Shim; Se Eun Kim; Kwangsik Jang Journal: In Vivo Date: 2022 Jul-Aug Impact factor: 2.406