Thiên-Nga Chamaraux-Tran1,2,3,4,5, Carole Mathelin2,3,4,5,6, Marc Aprahamian7, Girish P Joshi8, Catherine Tomasetto2,3,4,5, Pierre Diemunsch9,5,7, Cherif Akladios5,6,7. 1. Department of Anesthesiology and Surgical Intensive Care, Hautepierre Hospital, CHRU, University Hospital of Strasbourg, Strasbourg, France chamarau@igbmc.fr. 2. Functional Genomics and Cancer Department, Institute of Genetics and Molecular and Cellular Biology (IGBMC), Illkirch, France. 3. French National Center for Scientific Research (CNRS) UMR7104, Illkirch, France. 4. French National Institute of Health and Medical Research (INSERM) U964, Illkirch, France. 5. Faculty of Medicine, University of Strasbourg, Strasbourg, France. 6. Department of Gynecology and Obstetrics, Hautepierre Hospital, CHRU, University Hospital of Strasbourg, Strasbourg, France. 7. Research Institute against Digestive Cancer - European Institute of TeleSurgery (IRCAD), Strasbourg, France. 8. Department of Anesthesiology and Pain Management, University of Texas Southwestern Medical Center, Dallas, TX, U.S.A. 9. Department of Anesthesiology and Surgical Intensive Care, Hautepierre Hospital, CHRU, University Hospital of Strasbourg, Strasbourg, France.
Abstract
AIM: Retrospective studies have suggested a protective effect of regional anesthesia against recurrence after cancer surgery. But confirmation of the in vivo antitumor effects is lacking. We examined the in vitro antitumor effects of lidocaine on various breast cancer cell lines and then assessed these properties in vivo at clinically relevant concentrations. MATERIALS AND METHODS: In vitro experiments: normal breast epithelial cells (NBEC) MCF-10A and three tumor breast epithelial cells (TBEC) lines (MCF-7 luminal A, MDA-MB-231 triple-negative and SKBr3 HER2 positive) were exposed to increasing concentrations of lidocaine. Cell viability, migration and anchorage-independent growth were assessed by MTT, wound healing, and soft-agar growth assays. In vivo experiments: 6-week-old severe combined immunodeficient mice were injected intraperitoneally with MDA-MB-231 cells and were treated with intraperitoneal lidocaine or phosphate-buffered saline. The mice were euthanized when they reached experimental endpoints or sacrificed to determine peritoneal carcinomatosis index and global tumor volumes. RESULTS: Lidocaine reduced the viability of all the cell lines, inhibited migration of TBEC compared to the NBEC, and compromised the anchorage-independent growth of the triple-negative cells. Intraperitoneal lidocaine improved survival of mice with MDA-MB-231 peritoneal carcinomatosis using doses that are consistent with the current clinical settings for analgesia. CONCLUSION: In agreement with the notion that local anesthesia may be beneficial for cancer therapy, lidocaine has a protective effect against breast cancer cells in experimental studies. However, the beneficial impact of local anesthetics on breast cancer needs to be strengthened by additional preclinical and clinical trials. Copyright
AIM: Retrospective studies have suggested a protective effect of regional anesthesia against recurrence after cancer surgery. But confirmation of the in vivo antitumor effects is lacking. We examined the in vitro antitumor effects of lidocaine on various breast cancer cell lines and then assessed these properties in vivo at clinically relevant concentrations. MATERIALS AND METHODS: In vitro experiments: normal breast epithelial cells (NBEC) MCF-10A and three tumor breast epithelial cells (TBEC) lines (MCF-7 luminal A, MDA-MB-231 triple-negative and SKBr3 HER2 positive) were exposed to increasing concentrations of lidocaine. Cell viability, migration and anchorage-independent growth were assessed by MTT, wound healing, and soft-agar growth assays. In vivo experiments: 6-week-old severe combined immunodeficientmice were injected intraperitoneally with MDA-MB-231 cells and were treated with intraperitoneal lidocaine or phosphate-buffered saline. The mice were euthanized when they reached experimental endpoints or sacrificed to determine peritoneal carcinomatosis index and global tumor volumes. RESULTS:Lidocaine reduced the viability of all the cell lines, inhibited migration of TBEC compared to the NBEC, and compromised the anchorage-independent growth of the triple-negative cells. Intraperitoneal lidocaine improved survival of mice with MDA-MB-231 peritoneal carcinomatosis using doses that are consistent with the current clinical settings for analgesia. CONCLUSION: In agreement with the notion that local anesthesia may be beneficial for cancer therapy, lidocaine has a protective effect against breast cancer cells in experimental studies. However, the beneficial impact of local anesthetics on breast cancer needs to be strengthened by additional preclinical and clinical trials. Copyright
Authors: Beom Seok Han; Kyung Hee Jung; Ji Eun Lee; Young-Chan Yoon; Soyeon Ko; Min Seok Park; Yun Ji Lee; Sang Eun Kim; Ye Jin Cho; Pureunchowon Lee; Joo Han Lim; Eunsoo Jang; Hyunzu Kim; Soon-Sun Hong Journal: Am J Cancer Res Date: 2022-07-15 Impact factor: 5.942