Madoka Naemura1, Masahide Kuroki2, Toshiyuki Tsunoda3,4, Nagisa Arikawa1, Yuuga Sawata1, Senji Shirasawa3,4, Yojiro Kotake5,2. 1. Graduate School of Humanity-Oriented Science and Engineering, Faculty of Humanity-Oriented Science and Engineering, Kindai University, Iizuka, Japan. 2. Department of Biological and Environmental Chemistry, Faculty of Humanity-Oriented Science and Engineering, Kindai University, Iizuka, Japan. 3. Department of Cell Biology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan. 4. Central Research Institute for Advanced Molecular Medicine, Fukuoka University, Fukuoka, Japan. 5. Graduate School of Humanity-Oriented Science and Engineering, Faculty of Humanity-Oriented Science and Engineering, Kindai University, Iizuka, Japan ykotake@fuk.kindai.ac.jp.
Abstract
BACKGROUND/AIM: OPA-interacting protein 5 antisense transcript 1 (OIP5-AS1) is a long noncoding RNA located on human chromosome 15q15.1 and transcribed in the opposite direction to OIP5. Here, we report that OIP5-AS1 is involved in regulating cell proliferation. MATERIALS AND METHODS: HeLa cells were transfected with OIP5-AS1-targeting siRNA oligonucleotides and anti-sense oligonucleotides. The cells were harvested 72 h after transfection and subjected to quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and cell-cycle and apoptosis analysis. RESULTS: OIP5-AS1 was expressed at a lower level in cells harbouring an oncogenic kirsten rat sarcoma viral oncogene homolog (K-RAS) mutation than in cells expressing wild-type K-RAS. Silencing OIP5-AS1 with siRNA oligonucleotides or anti-sense oligonucleotides reduced HeLa cell proliferation. Apoptosis and cell-cycle analysis showed that silencing OIP5-AS1 did not cause apoptosis, but did cause G2/M phase cell-cycle arrest. CONCLUSION: These results suggest that OIP5-AS1 positively regulates cell proliferation by promoting G2/M phase progression. Copyright
BACKGROUND/AIM: OPA-interacting protein 5 antisense transcript 1 (OIP5-AS1) is a long noncoding RNA located on human chromosome 15q15.1 and transcribed in the opposite direction to OIP5. Here, we report that OIP5-AS1 is involved in regulating cell proliferation. MATERIALS AND METHODS: HeLa cells were transfected with OIP5-AS1-targeting siRNA oligonucleotides and anti-sense oligonucleotides. The cells were harvested 72 h after transfection and subjected to quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and cell-cycle and apoptosis analysis. RESULTS:OIP5-AS1 was expressed at a lower level in cells harbouring an oncogenic kirsten ratsarcoma viral oncogene homolog (K-RAS) mutation than in cells expressing wild-type K-RAS. Silencing OIP5-AS1 with siRNA oligonucleotides or anti-sense oligonucleotides reduced HeLa cell proliferation. Apoptosis and cell-cycle analysis showed that silencing OIP5-AS1 did not cause apoptosis, but did cause G2/M phase cell-cycle arrest. CONCLUSION: These results suggest that OIP5-AS1 positively regulates cell proliferation by promoting G2/M phase progression. Copyright