Guanqun Hu1, Mingjie Zhang2, Min Su1, Qing Zhang1, Hangfei Wu1, Xiaolin Wang3, Zhao Dong3, Shengyuan Yu4. 1. Department of Neurology, Chinese PLA General Hospital, Beijing 100853, China; School of Medicine, Nankai University, Tianjin, 300071, China. 2. Department of Neurology, Chinese PLA General Hospital, Beijing 100853, China. Electronic address: mjzhangnk@163.com. 3. Department of Neurology, Chinese PLA General Hospital, Beijing 100853, China. 4. Department of Neurology, Chinese PLA General Hospital, Beijing 100853, China. Electronic address: yusy1963@126.com.
Abstract
BACKGROUND: To identify differences in allodynia and grooming behaviours between rat models of either repeated dural nociception with inflammatory soup (IS) or infraorbital nerve chronic constriction injury (IoN-CCI). METHODS: Repeated dural nociception was induced via the application of IS to the dural meninges and IoN-CCI was applied to model neuropathic pain. All surgeries were performed on the right side and a sham operation was performed on the control group. Mechanical and thermal withdrawal thresholds were tested on different facial areas and hindpaw during the interictal period and grooming behaviours were recorded. RESULTS: A significant decreases was found in the mechanical withdrawal thresholds of the bilateral vibrissa pad and right periorbital area in both the IS and the IoN-CCI groups, but only in the left periorbital area of the IS group. Hindpaw thermal allodynia was evident only in the IS group. Ipsilateral hindpaw grooming behaviour increased in the IS group and facial grooming behaviour increased in the IoN-CCI group. CONCLUSIONS: Repeated dural nociception induced by IS and IoN-CCI in rats effectively simulated chronic migraine (CM) and trigeminal neuralgia (TN), respectively. The IS group exhibited a wider range of allodynia than the IoN-CCI group, but further studies are necessary to determine underlying mechanisms.
BACKGROUND: To identify differences in allodynia and grooming behaviours between rat models of either repeated dural nociception with inflammatory soup (IS) or infraorbital nerve chronic constriction injury (IoN-CCI). METHODS: Repeated dural nociception was induced via the application of IS to the dural meninges and IoN-CCI was applied to model neuropathic pain. All surgeries were performed on the right side and a sham operation was performed on the control group. Mechanical and thermal withdrawal thresholds were tested on different facial areas and hindpaw during the interictal period and grooming behaviours were recorded. RESULTS: A significant decreases was found in the mechanical withdrawal thresholds of the bilateral vibrissa pad and right periorbital area in both the IS and the IoN-CCI groups, but only in the left periorbital area of the IS group. Hindpaw thermal allodynia was evident only in the IS group. Ipsilateral hindpaw grooming behaviour increased in the IS group and facial grooming behaviour increased in the IoN-CCI group. CONCLUSIONS: Repeated dural nociception induced by IS and IoN-CCI in rats effectively simulated chronic migraine (CM) and trigeminal neuralgia (TN), respectively. The IS group exhibited a wider range of allodynia than the IoN-CCI group, but further studies are necessary to determine underlying mechanisms.