Literature DB >> 29277486

Development of a high-throughput bead based assay system to measure HIV-1 specific immune signatures in clinical samples.

Thomas Liechti1, Claus Kadelka2, Hanna Ebner3, Nikolas Friedrich4, Roger D Kouyos5, Huldrych F Günthard6, Alexandra Trkola7.   

Abstract

The monitoring and assessment of a broadly neutralizing antibody (bnAb) based HIV-1 vaccine require detailed measurements of HIV-1 binding antibody responses to support the detection of correlates of protection. Here we describe the development of a flexible, high-throughput microsphere based multiplex assay system that allows monitoring complex binding antibody signatures. Studying a panel of 13 HIV-1 antigens in a parallel assessment of different IgG subclasses (IgG1, IgG2 and IgG3) we demonstrate the potential of our strategy. The technical advances we describe include means to improve antigen reactivity using directed neutravidin-biotin immobilization of antigens and biotin saturation to reduce background. A particular emphasis of our study was to provide tools for the assessment of reproducibility and stability of the assay system and strategies to control for variations allowing the application in high-throughput assays, where reliability of single measurements needs to be guaranteed.
Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Entities:  

Keywords:  HIV-1 binding antibody response; High-throughput screen; Microsphere based multiplex antigen binding assay

Mesh：

Substances：

Year:  2017        PMID： 29277486     DOI： 10.1016/j.jim.2017.12.003

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  7 in total

1.  Distinct, IgG1-driven antibody response landscapes demarcate individuals with broadly HIV-1 neutralizing activity.

Authors:  Claus Kadelka; Thomas Liechti; Hanna Ebner; Merle Schanz; Peter Rusert; Nikolas Friedrich; Emanuel Stiegeler; Dominique L Braun; Michael Huber; Alexandra U Scherrer; Jacqueline Weber; Therese Uhr; Herbert Kuster; Benjamin Misselwitz; Matthias Cavassini; Enos Bernasconi; Matthias Hoffmann; Alexandra Calmy; Manuel Battegay; Andri Rauch; Sabine Yerly; Vincent Aubert; Thomas Klimkait; Jürg Böni; Roger D Kouyos; Huldrych F Günthard; Alexandra Trkola
Journal:  J Exp Med       Date:  2018-05-24       Impact factor: 14.307

2.  Widespread B cell perturbations in HIV-1 infection afflict naive and marginal zone B cells.

Authors:  Thomas Liechti; Claus Kadelka; Dominique L Braun; Herbert Kuster; Jürg Böni; Melissa Robbiani; Huldrych F Günthard; Alexandra Trkola
Journal:  J Exp Med       Date:  2019-06-20       Impact factor: 14.307

3.  A comparison of non-magnetic and magnetic beads for measuring IgG antibodies against Plasmodium vivax antigens in a multiplexed bead-based assay using Luminex technology (Bio-Plex 200 or MAGPIX).

Authors:  Ramin Mazhari; Jessica Brewster; Rich Fong; Caitlin Bourke; Zoe S J Liu; Eizo Takashima; Takafumi Tsuboi; Wai-Hong Tham; Matthias Harbers; Chetan Chitnis; Julie Healer; Maria Ome-Kaius; Jetsumon Sattabongkot; James Kazura; Leanne J Robinson; Christopher King; Ivo Mueller; Rhea J Longley
Journal:  PLoS One       Date:  2020-12-04       Impact factor: 3.240

4.  Multifactorial seroprofiling dissects the contribution of pre-existing human coronaviruses responses to SARS-CoV-2 immunity.

Authors:  Irene A Abela; Chloé Pasin; Magdalena Schwarzmüller; Selina Epp; Michèle E Sickmann; Merle M Schanz; Peter Rusert; Jacqueline Weber; Stefan Schmutz; Annette Audigé; Liridona Maliqi; Annika Hunziker; Maria C Hesselman; Cyrille R Niklaus; Jochen Gottschalk; Eméry Schindler; Alexander Wepf; Urs Karrer; Aline Wolfensberger; Silvana K Rampini; Patrick M Meyer Sauteur; Christoph Berger; Michael Huber; Jürg Böni; Dominique L Braun; Maddalena Marconato; Markus G Manz; Beat M Frey; Huldrych F Günthard; Roger D Kouyos; Alexandra Trkola
Journal:  Nat Commun       Date:  2021-11-18       Impact factor: 14.919

5.  Distinct conformations of the HIV-1 V3 loop crown are targetable for broad neutralization.

Authors:  Nikolas Friedrich; Emanuel Stiegeler; Matthias Glögl; Thomas Lemmin; Simon Hansen; Claus Kadelka; Yufan Wu; Patrick Ernst; Liridona Maliqi; Caio Foulkes; Mylène Morin; Mustafa Eroglu; Thomas Liechti; Branislav Ivan; Thomas Reinberg; Jonas V Schaefer; Umut Karakus; Stephan Ursprung; Axel Mann; Peter Rusert; Roger D Kouyos; John A Robinson; Huldrych F Günthard; Andreas Plückthun; Alexandra Trkola
Journal:  Nat Commun       Date:  2021-11-18       Impact factor: 14.919

6.  Critically ill COVID-19 patients with neutralizing autoantibodies against type I interferons have increased risk of herpesvirus disease.

Authors:  Idoia Busnadiego; Irene A Abela; Pascal M Frey; Daniel A Hofmaenner; Thomas C Scheier; Reto A Schuepbach; Philipp K Buehler; Silvio D Brugger; Benjamin G Hale
Journal:  PLoS Biol       Date:  2022-07-05       Impact factor: 9.593

Review 7.  Rapid Response to Pandemic Threats: Immunogenic Epitope Detection of Pandemic Pathogens for Diagnostics and Vaccine Development Using Peptide Microarrays.

Authors:  Kirsten Heiss; Jasmin Heidepriem; Nico Fischer; Laura K Weber; Christine Dahlke; Thomas Jaenisch; Felix F Loeffler
Journal:  J Proteome Res       Date:  2020-09-21       Impact factor: 4.466
  7 in total

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