Raquel Muñoz-Fernández1, Claudia de la Mata1, Alejandro Prados1, Ana Perea1, María José Ruiz-Magaña1, Tatiana Llorca1, Pablo Fernández-Rubio1, Osmany Blanco2, Ana C Abadía-Molina3, Enrique G Olivares4. 1. Instituto de Biopatología y Medicina Regenerativa, Centro de Investigación Biomédica, Universidad de Granada, Armilla, Granada, Spain. 2. Bacteriología y Laboratorio Clínico, Facultad de Salud, Universidad de Santander, Bucaramanga, Colombia. 3. Instituto de Biopatología y Medicina Regenerativa, Centro de Investigación Biomédica, Universidad de Granada, Armilla, Granada, Spain; Departamento de Bioquímica y Biología Molecular III e Inmunología, Universidad de Granada, Granada, Spain. 4. Instituto de Biopatología y Medicina Regenerativa, Centro de Investigación Biomédica, Universidad de Granada, Armilla, Granada, Spain; Departamento de Bioquímica y Biología Molecular III e Inmunología, Universidad de Granada, Granada, Spain; Unidad de Gestión Clínica Laboratorios, Complejo Hospitalario Universitario de Granada, Granada, Spain. Electronic address: engarcia@ugr.es.
Abstract
INTRODUCTION: Human decidual stromal cells (DSCs) play a key role in maternal-fetal interactions. Precursors of DSCs (preDSCs) localize around vessels in both the endometrium and decidua. Previous studies suggested a relationship between preDSCs and pericytes because these cells share a perivascular location, alpha smooth muscle actin (α-SM actin) expression and the ability to contract under the effects of cytokines. METHODS: To further study this relationship, we established 15 human preDSC lines and 3 preDSC clones. The preDSC lines and clones were tested by flow cytometry with a panel of 29 monoclonal antibodies, 14 of which are pericyte markers. The expression of angiogenic factors was determined by RT-PCR, chemotactic activity was studied with the migration assay, and cell contractility was evaluated with the collagen cell contraction assay. Confocal microscopy was used to study decidual sections. RESULTS: Under the effect of progesterone and cAMP, these lines decidualized in vitro: the cells became rounder and secreted prolactin, a marker of physiological DSC differentiation (decidualization). The antigen phenotype of these preDSC lines and clones was fully compatible with that reported for pericytes. PreDSC lines displayed pericyte characteristics: they expressed angiogenic factors and showed chemotactic and cytokine-induced contractile activity. Confocal microscopic examination of decidual sections revealed the expression of antigens detected in preDSC lines: α-SM actin colocalized with CD146, CD140b, MFG-E8, nestin, and STRO-1 (all of which are pericyte markers) in cells located around the vessels, a distinctive location of preDSCs and pericytes. DISCUSSION: Taken together, our results show that preDSCs are pericyte-like cells.
INTRODUCTION:Human decidual stromal cells (DSCs) play a key role in maternal-fetal interactions. Precursors of DSCs (preDSCs) localize around vessels in both the endometrium and decidua. Previous studies suggested a relationship between preDSCs and pericytes because these cells share a perivascular location, alpha smooth muscle actin (α-SM actin) expression and the ability to contract under the effects of cytokines. METHODS: To further study this relationship, we established 15 human preDSC lines and 3 preDSC clones. The preDSC lines and clones were tested by flow cytometry with a panel of 29 monoclonal antibodies, 14 of which are pericyte markers. The expression of angiogenic factors was determined by RT-PCR, chemotactic activity was studied with the migration assay, and cell contractility was evaluated with the collagen cell contraction assay. Confocal microscopy was used to study decidual sections. RESULTS: Under the effect of progesterone and cAMP, these lines decidualized in vitro: the cells became rounder and secreted prolactin, a marker of physiological DSC differentiation (decidualization). The antigen phenotype of these preDSC lines and clones was fully compatible with that reported for pericytes. PreDSC lines displayed pericyte characteristics: they expressed angiogenic factors and showed chemotactic and cytokine-induced contractile activity. Confocal microscopic examination of decidual sections revealed the expression of antigens detected in preDSC lines: α-SM actin colocalized with CD146, CD140b, MFG-E8, nestin, and STRO-1 (all of which are pericyte markers) in cells located around the vessels, a distinctive location of preDSCs and pericytes. DISCUSSION: Taken together, our results show that preDSCs are pericyte-like cells.
Authors: Roser Vento-Tormo; Mirjana Efremova; Muzlifah Haniffa; Ashley Moffett; Sarah A Teichmann; Rachel A Botting; Margherita Y Turco; Miquel Vento-Tormo; Kerstin B Meyer; Jong-Eun Park; Emily Stephenson; Krzysztof Polański; Angela Goncalves; Lucy Gardner; Staffan Holmqvist; Johan Henriksson; Angela Zou; Andrew M Sharkey; Ben Millar; Barbara Innes; Laura Wood; Anna Wilbrey-Clark; Rebecca P Payne; Martin A Ivarsson; Steve Lisgo; Andrew Filby; David H Rowitch; Judith N Bulmer; Gavin J Wright; Michael J T Stubbington Journal: Nature Date: 2018-11-14 Impact factor: 69.504
Authors: Raquel Muñoz-Fernández; Claudia De La Mata; Francisco Requena; Francisco Martín; Pablo Fernandez-Rubio; Tatiana Llorca; Maria José Ruiz-Magaña; Carmen Ruiz-Ruiz; Enrique G Olivares Journal: Stem Cell Res Ther Date: 2019-06-14 Impact factor: 6.832