Draft genome sequence of Anoxybacillus flavithermus KU2-6-11 isolated from hot-spring in Uzon caldera (Kamchatka, Russia).

The Anoxybacillus flavithermus KU2-6-11 was isolated from sediments of a nameless hot spring. The hot spring is located in Uzon caldera (Kamchatka, Russia). The sequenced and annotated genome is 2,646,305 bp and encodes 2787genes. The draft genome sequence of the Anoxybacillus flavithermus KU2-6-11 has been deposited at DDBJ/EMBL/GenBank under the accession PEDM01000000 and the sequences could be found at the site https://www.ncbi.nlm.nih.gov/nuccore/PEDM01000000.

Specifications

Value of the data

Genome Anoxybacillus flavithermus consist genes important for biotechnology.

Draft genome assembly of Anoxybacillus flavithermus, from poorly studied region, will increase the knowledge of the biochemical process of thermophilic communities and create an opportunity for comparative studies with other bacteria.

Data

Anoxybacillus was defined as a new genus in 2000, the first type strain was A. pushchinoensis [1]. In this work, Bacillus flavithermus was reclassified as Anoxybacillus flavithermus [1]. The members of this genus are alkaliphilic, moderately thermophilic, spore-forming, Gram-positive bacteria belonging to the order of Bacillales. Present time the genus Anoxybacillus include more 15 species [1], [2], [3], [4], [5]. Species of this genus are widely distributed in geothermal springs [1], [6]. In Russia they were detected in geothermal springs of Baikal rift zone [7], in the Geyser valley, Kamchatka [8].

Anoxybacillus flavithermus strain KU2-6-11 was isolated from cyanobacterial mat from nameless hot spring (65 °C) in Uzon Caldera (Kamchatka, Russia, 54о 29.883' N; 160о 00.874' E), 10 m from Zavarzin hot spring [9]. The hot spring has diameter of less than 1 m. The threads of sulfur bacteria developing on the edge of the funnel and outflow of the stream by, replaced by cyano and algo-bacterial communities. We isolated the strain for Collection of biotechnological microorganisms as a source of novel promising objects for biotechnology and bioengineering of Federal Research Center "Institute of Cytology and Genetics of the Siberian Branch of the RAS".

The draft genome sequence for Anoxybacillus flavithermus strain KU2-6-11 has been deposited in DDBJ/EMBL/Genbank under the accession no. PEDM01000000. The 74 contigs have been deposited under accession no. PEDM01000001–PEDM01000074.

Experimental design, materials, and methods

Library

Strategy: Whole-genome DNA sequencing.

Taxon: Anoxybacillus flavithermus.

Sample details: The strain was isolated in 2011 by Alla V. Bryanskaya and stored at Collection of biotechnological microorganisms as a source of novel promising objects for biotechnology and bioengineering of Federal Research Center "Institute of Cytology and Genetics of the Siberian Branch of the RAS".

Location: Uzon Caldera (Kamchatka, Russia), 10 m from Zavarzin hot spring. (54о 29.883' N; 160о 00.874' E).

Sample handling: culture was cultivated in liquid medium containing 1% trypton, 0.5% yeast extract, and 1%of NaCl. DNA was extracted from 8 ml using the DNA Purification Kit (Fermentas).

Library: Paired-end 75 bp reads.

DNA isolation and sequencing

A.flavithermus KU2-6-11 culture was cultivated in liquid medium containing 1% trypton, 0.5% yeast extract, and 1%of NaCl. Eight ml of cell culture were pelleted by centrifugation and resuspended in 75 µl of H2O by intense pipetting. DNA was isolated using the DNA Purification Kit (Fermentas). Nebnext® ultra™ II DNA library prep kit for Illumina (New England Biolabs, USA) were used to create libraries for genome sequencing. Genome sequencing was performed on MiSeq (Illumina), using MiSeq Reagent Kit v3 (150-cycle) (Illumina, USA) in the "Molecular and cellular biology" facility at IMCB SB RAS.

Data processing

De novo assembly of short reads into contigs was performed using SPAdes v. 3.10.1 [10]. Contigs shorter than 1000 bp were deleted. A total of 74 contigs yielded a genome sequence 2,652,077 bp long, and the G + C content is 41.48%. ORF prediction and automatic annotation was performed using NCBI PGAAP (http://www.ncbi.nlm.nih.gov/genome/annotation_prok). The complete genome sequence contained 2787 genes, 2673 CDS, rRNAs ( 5 - 5S, 1 - 16S, 6 - 23S), 29 tRNAs, 4 ncRNA.

Subject area	Biology
More specific subject area	Microbiology, Genomics
Type of data	Genomic sequence, gene prediction of Anoxybacillus flavithermus
How data was acquired	Shotgun whole-genome DNA sequencing using MiSeq platform (Illumina) at the "Molecular and cellular biology" facility at IMCB SB RAS
Data format	Draft genome assembly, and gene prediction
Experimental factors	DNA was extracted from Anoxybacillus flavithermus KU2-6-11
Experimental features	Whole genome shotgun sequencing followed by genome assembly and gene description
Data source location	A. flavithermus KU2-6-11 was isolated from a cyanobacteria mat from nameless hot spring (65 °C) in Uzon Caldera (Kamchatka, Russia), 10 m from Zavarzin hot spring. (54о 29.883' N; 160о 00.874' E)
Data accessibility	The draft genome sequence for Anoxybacillus flavithermus strain KU2-6-11 has been deposited in DDBJ/EMBL/Genbank under the accession no. PEDM01000000. The 74 contigs have been deposited under accession no. PEDM01000001–PEDM01000074.
	https://www.ncbi.nlm.nih.gov/nuccore/PEDM01000000