Literature DB >> 29276726

Drosophila Model of Leishmania amazonensis Infection.

Kendi Okuda1, Neal Silverman1.   

Abstract

This protocol describes how to generate and harvest antibody-free L. amazonensis amastigotes, and how to infect adult Drosophila melanogaster with these parasites. This model recapitulates key aspects of the interactions between Leishmania amastigotes and animal phagocytes.

Entities:  

Keywords:  Amastigote; Axenic amastigote; Drosophila melanogaster; Hemocytes; Leishmania; Phagocytosis; Plasmatocytes; RNAi screen

Year:  2017        PMID： 29276726      PMCID： PMC5738924          DOI： 10.21769/BioProtoc.2640

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  6 in total

1.  Genetic ablation of Drosophila phagocytes reveals their contribution to both development and resistance to bacterial infection.

Authors:  Arnaud Defaye; Iwan Evans; Michèle Crozatier; Will Wood; Bruno Lemaitre; François Leulier
Journal:  J Innate Immun       Date:  2009-04-01       Impact factor: 7.349

2.  Photodynamic sensitization of Leishmania amazonensis in both extracellular and intracellular stages with aluminum phthalocyanine chloride for photolysis in vitro.

Authors:  Sujoy Dutta; Debalina Ray; Bala K Kolli; Kwang-Poo Chang
Journal:  Antimicrob Agents Chemother       Date:  2005-11       Impact factor: 5.191

Review 3.  In vitro cultivation and characterization of axenic amastigotes of Leishmania.

Authors:  N Gupta; N Goyal; A K Rastogi
Journal:  Trends Parasitol       Date:  2001-03

4.  Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.

Authors:  A H Brand; N Perrimon
Journal:  Development       Date:  1993-06       Impact factor: 6.868

5.  CellProfiler: image analysis software for identifying and quantifying cell phenotypes.

Authors:  Anne E Carpenter; Thouis R Jones; Michael R Lamprecht; Colin Clarke; In Han Kang; Ola Friman; David A Guertin; Joo Han Chang; Robert A Lindquist; Jason Moffat; Polina Golland; David M Sabatini
Journal:  Genome Biol       Date:  2006-10-31       Impact factor: 13.583

6.  Leishmania amazonensis Engages CD36 to Drive Parasitophorous Vacuole Maturation.

Authors:  Kendi Okuda; Mei Tong; Brian Dempsey; Kathryn J Moore; Ricardo T Gazzinelli; Neal Silverman
Journal:  PLoS Pathog       Date:  2016-06-09       Impact factor: 6.823
  6 in total

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