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Literature DB >> 29276723

Cell-free Generation of COPII-coated Procollagen I Carriers.

Lin Yuan¹, Satoshi Baba¹, Kanika Bajaj¹, Randy Schekman¹.

Abstract

The aim of this protocol is to generate COPII-coated procollagen I (PC1) carriers in a cell-free reaction. The COPII-coated PC1 carriers were reconstituted from donor membrane, cytosol, purified recombinant COPII proteins, and nucleotides. This protocol describes the preparation of donor membrane and cytosol, the assembly of the reaction, and the isolation and detection of reconstituted COPII-coated carriers. This cell-free reaction can be used to test conditions that stimulate or suppress the packaging of PC1 into COPII-coated carriers.

Entities: Chemical

Keywords: Budding; COPII; Collagen; Membrane; Reconstitution

Year: 2017 PMID： 29276723 PMCID： PMC5738930 DOI： 10.21769/BioProtoc.2450

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

7 in total

Review 1. COPII-coated vesicles: flexible enough for large cargo?

Authors: J Christopher Fromme; Randy Schekman
Journal: Curr Opin Cell Biol Date: 2005-08 Impact factor: 8.382

2. COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum.

Authors: C Barlowe; L Orci; T Yeung; M Hosobuchi; S Hamamoto; N Salama; M F Rexach; M Ravazzola; M Amherdt; R Schekman
Journal: Cell Date: 1994-06-17 Impact factor: 41.582

3. Order of events in the yeast secretory pathway.

Authors: P Novick; S Ferro; R Schekman
Journal: Cell Date: 1981-08 Impact factor: 41.582

4. Cranio-lenticulo-sutural dysplasia is caused by a SEC23A mutation leading to abnormal endoplasmic-reticulum-to-Golgi trafficking.

Authors: Simeon A Boyadjiev; J Christopher Fromme; Jin Ben; Samuel S Chong; Christopher Nauta; David J Hur; George Zhang; Susan Hamamoto; Randy Schekman; Mariella Ravazzola; Lelio Orci; Wafaa Eyaid
Journal: Nat Genet Date: 2006-09-17 Impact factor: 38.330

5. Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway.

Authors: C A Kaiser; R Schekman
Journal: Cell Date: 1990-05-18 Impact factor: 41.582

6. Uncoupled packaging of amyloid precursor protein and presenilin 1 into coat protein complex II vesicles.

Authors: Jinoh Kim; Susan Hamamoto; Mariella Ravazzola; Lelio Orci; Randy Schekman
Journal: J Biol Chem Date: 2004-12-28 Impact factor: 5.157

7. COPII-coated membranes function as transport carriers of intracellular procollagen I.

Authors: Amita Gorur; Lin Yuan; Samuel J Kenny; Satoshi Baba; Ke Xu; Randy Schekman
Journal: J Cell Biol Date: 2017-04-20 Impact factor: 10.539

7 in total

6 in total

1. YIPF6 controls sorting of FGF21 into COPII vesicles and promotes obesity.

Authors: Lirui Wang; Magdalena Mazagova; Chuyue Pan; Song Yang; Katharina Brandl; Jun Liu; Shannon M Reilly; Yanhan Wang; Zhaorui Miao; Rohit Loomba; Na Lu; Qinglong Guo; Jihua Liu; Ruth T Yu; Michael Downes; Ronald M Evans; David A Brenner; Alan R Saltiel; Bruce Beutler; Bernd Schnabl
Journal: Proc Natl Acad Sci U S A Date: 2019-07-09 Impact factor: 11.205

Cell-free Generation of COPII-coated Procollagen I Carriers.

Review 1. COPII-coated vesicles: flexible enough for large cargo?

2. COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum.

3. Order of events in the yeast secretory pathway.

4. Cranio-lenticulo-sutural dysplasia is caused by a SEC23A mutation leading to abnormal endoplasmic-reticulum-to-Golgi trafficking.

5. Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway.

6. Uncoupled packaging of amyloid precursor protein and presenilin 1 into coat protein complex II vesicles.

7. COPII-coated membranes function as transport carriers of intracellular procollagen I.

1. YIPF6 controls sorting of FGF21 into COPII vesicles and promotes obesity.

2. Cell-free Reconstitution of the Packaging of Cargo Proteins into Vesicles at the trans Golgi Network.

3. TANGO1 membrane helices create a lipid diffusion barrier at curved membranes.

Review 4. COPII-dependent ER export in animal cells: adaptation and control for diverse cargo.

5. ER-to-Golgi trafficking of procollagen in the absence of large carriers.

6. TANGO1 and SEC12 are copackaged with procollagen I to facilitate the generation of large COPII carriers.