Christina Stene1, Andrea Polistena2, Alexander Gaber3, Björn Nodin3, Bianca Ottochian4, Diya Adawi4, Nicola Avenia2, Karin Jirström5, Louis Banka Johnson6. 1. Division of Colorectal Surgery, Department of Clinical Sciences, Skåne University Hospital/Malmö, Lund University, Malmö, Sweden. 2. General and Specialist Surgery Unit, S. Maria University Hospital, Perugia University, Terni, Italy. 3. Division of Pathology, Department of Clinical Sciences, Skåne University Hospital/Malmö, Lund University, Malmö, Sweden. 4. Division of Surgery, Department of Clinical Sciences, Skåne University Hospital/Malmö, Lund University, Malmö, Sweden. 5. Division of Oncology and Pathology, Department of Clinical Sciences, Lund University, Malmö, Sweden. 6. Division of Colorectal Surgery, Department of Clinical Sciences, Skåne University Hospital/Malmö, Lund University, Malmö, Sweden louis.johnson@med.lu.se.
Abstract
BACKGROUND/AIM: Matrix metalloproteinase 7 (MMP7) expression is highly associated with colorectal cancer and modulates tumour growth and invasion. Radiation injury induces inflammation with increases in MMP7 and in transforming growth factor beta (TGFβ). The aim of this study was to investigate the effect on MMP7 and TGFβ. expression in patients with rectal cancer undergoing different regimens of neoadjuvant radiotherapy (RT). PATIENTS AND METHODS: We studied 53 patients in three RT treatment groups receiving RT of 25 Gy, long-term RT 50 Gy and controls receiving no RT. Three biopsies were obtained from each patient during the treatments: before RT, after RT and after surgery. Tissue samples were formalin fixed, paraffin embedded and tissue microarrays were constructed and stained for MMP7 and TGFβ. Mann-Whitney U-tests and Wilcoxon Z-tests were used to determine differences between patients before and after RT, and after surgery, as well as between the RT groups. RESULTS: In all three patient groups, increases of MMP7 and TGFβ expression were observed after surgery. MMP7 expression was significantly increased in patients receiving short-term RT but TGFβ expression was not affected by RT. CONCLUSION: 50 Gy Irradiation of rectal cancer gives less tumour activation of MMP7, whilst it is up-regulated by 25 Gy and surgery regardless of RT. Copyright
BACKGROUND/AIM: Matrix metalloproteinase 7 (MMP7) expression is highly associated with colorectal cancer and modulates tumour growth and invasion. Radiation injury induces inflammation with increases in MMP7 and in transforming growth factor beta (TGFβ). The aim of this study was to investigate the effect on MMP7 and TGFβ. expression in patients with rectal cancer undergoing different regimens of neoadjuvant radiotherapy (RT). PATIENTS AND METHODS: We studied 53 patients in three RT treatment groups receiving RT of 25 Gy, long-term RT 50 Gy and controls receiving no RT. Three biopsies were obtained from each patient during the treatments: before RT, after RT and after surgery. Tissue samples were formalin fixed, paraffin embedded and tissue microarrays were constructed and stained for MMP7 and TGFβ. Mann-Whitney U-tests and Wilcoxon Z-tests were used to determine differences between patients before and after RT, and after surgery, as well as between the RT groups. RESULTS: In all three patient groups, increases of MMP7 and TGFβ expression were observed after surgery. MMP7 expression was significantly increased in patients receiving short-term RT but TGFβ expression was not affected by RT. CONCLUSION: 50 Gy Irradiation of rectal cancer gives less tumour activation of MMP7, whilst it is up-regulated by 25 Gy and surgery regardless of RT. Copyright
Authors: Andrea Polistena; Alessandra Cucina; Simona Dinicola; Christina Stene; Giuseppe Cavallaro; Antonio Ciardi; Gennaro Orlando; Rossella Arena; Giuseppe D'Ermo; Antonino Cavallaro; Louis Banka Johnson; Giorgio De Toma Journal: In Vivo Date: 2014 Jan-Feb Impact factor: 2.155
Authors: Alejandro Martínez-Fernandez; Xabier García-Albeniz; Estela Pineda; Laura Visa; Rosa Gallego; Jordi Codony-Servat; Josep Maria Augé; Raquel Longarón; Pere Gascón; Antonio Lacy; Antoni Castells; Joan Maurel Journal: Ann Surg Oncol Date: 2009-03-04 Impact factor: 5.344