Gabriela Marini1, Fernanda Piculo2, Giovana Vesentini2, Débora C Damasceno2, Flávia K Delella3, Iracema M P Calderon2, Firouz Daneshgari4, Sérgio L Felisbino3, Angélica M P Barbosa2, Marilza V C Rudge2. 1. Universidade do Sagrado Coração (USC), Bauru, São Paulo, Brazil; UNESP - Univ Estadual Paulista, Botucatu Medical School, Department of Gynecology and Obstetrics, Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu, São Paulo, Brazil. Electronic address: gabriela.marini@usc.br. 2. UNESP - Univ Estadual Paulista, Botucatu Medical School, Department of Gynecology and Obstetrics, Laboratory of Experimental Research on Gynecology and Obstetrics, Botucatu, São Paulo, Brazil. 3. UNESP - Univ Estadual Paulista, Institute of Biosciences, Department of Morphology, Botucatu, São Paulo, Brazil. 4. Case Western Reserve University, Department of Urology, Cleveland, OH, USA.
Abstract
OBJECTIVE: To analyze the distribution and quantification of the key structural extracellular matrix components of the urethral tissue in a rat model of hyperglycemia and pregnancy. STUDY DESIGN: A total of 120 female Wistar rats were distributed into the following four experimental groups: virgin, pregnant, hyperglycemic and hyperglycemic + pregnant groups. The urethra was harvested for histochemical, morphometric, immunohistochemical, Western blot and glycosaminoglycan analyses. All protocols were approved by the Institutional Animal Care and Use Committee of Botucatu Medical School (process number 828-2010). RESULTS: The hyperglycemic + pregnant group showed significantly increased stiffness in urethral tissue. The total striated muscle was decreased, with increased deposition of collagen fibers around the muscle fibers and a change in the organization of the collagen fibrils. An increase in the relative collagen type I/III ratio and a decrease in total glycosaminoglycans were also observed. CONCLUSIONS: This study provides the first line of experimental evidence supporting a metabolic relationship between hyperglycemia and urethral remodeling of connective tissue in pregnant rats. The different organization of the collagen fibrils and the profile of glycosaminoglycans found in urethral samples suggest that the pathology of the urethral fibromuscular system could be related to hyperglycemia-induced pelvic floor dysfunction in women, which has direct clinical implications with the possibility to develop new multidisciplinary treatments for improving the health care of these women.
OBJECTIVE: To analyze the distribution and quantification of the key structural extracellular matrix components of the urethral tissue in a rat model of hyperglycemia and pregnancy. STUDY DESIGN: A total of 120 female Wistar rats were distributed into the following four experimental groups: virgin, pregnant, hyperglycemic and hyperglycemic + pregnant groups. The urethra was harvested for histochemical, morphometric, immunohistochemical, Western blot and glycosaminoglycan analyses. All protocols were approved by the Institutional Animal Care and Use Committee of Botucatu Medical School (process number 828-2010). RESULTS: The hyperglycemic + pregnant group showed significantly increased stiffness in urethral tissue. The total striated muscle was decreased, with increased deposition of collagen fibers around the muscle fibers and a change in the organization of the collagen fibrils. An increase in the relative collagen type I/III ratio and a decrease in total glycosaminoglycans were also observed. CONCLUSIONS: This study provides the first line of experimental evidence supporting a metabolic relationship between hyperglycemia and urethral remodeling of connective tissue in pregnant rats. The different organization of the collagen fibrils and the profile of glycosaminoglycans found in urethral samples suggest that the pathology of the urethral fibromuscular system could be related to hyperglycemia-induced pelvic floor dysfunction in women, which has direct clinical implications with the possibility to develop new multidisciplinary treatments for improving the health care of these women.
Authors: G Vesentini; G Marini; F Piculo; D C Damasceno; S M M Matheus; S L Felisbino; I M P Calderon; A Hijaz; A M P Barbosa; M V C Rudge Journal: Braz J Med Biol Res Date: 2018-03-01 Impact factor: 2.590
Authors: Bruna B Catinelli; Patrícia S Rossignoli; Juliana F Floriano; Aline M Carr; Rafael G de Oliveira; Nilton J Dos Santos; Lara C C Úbeda; Maria Angélica Spadella; Raghavendra L S Hallur; Luis Sobrevia; Sérgio L Felisbino; Iracema M P Calderon; Angélica M P Barbosa; Marilza V C Rudge Journal: Sci Rep Date: 2022-05-05 Impact factor: 4.996
Authors: David R A Reyes; Angelica M P Barbosa; Marilza V C Rudge; Iracema I M P Calderon; Floriano F Juliana; Quiroz B C V Sofia; Sarah M B Costa; Raghavendra L S Hallur; Eusebio M A Enriquez; Rafael G Oliveira; Patricia de Souza Rossignolli; Cristiane Rodrigues Pedroni; Fernanda C B Alves; Gabriela A Garcia; Joelcio F Abbade; Carolina N F Carvalho; Luis Sobrevia Journal: Biomed Eng Online Date: 2022-10-14 Impact factor: 3.903
Authors: Giovana Vesentini; Angélica M P Barbosa; Débora C Damasceno; Gabriela Marini; Fernanda Piculo; Selma M M Matheus; Raghavendra L S Hallur; Sthefanie K Nunes; Bruna B Catinelli; Claudia G Magalhães; Roberto Costa; Joelcio F Abbade; José E Corrente; Iracema M P Calderon; Marilza V C Rudge Journal: PLoS One Date: 2020-04-03 Impact factor: 3.240