Ghareib W Ali1, W El-Hotaby2, Bahaa Hemdan3, Wafa I Abdel-Fattah4. 1. Refractories, Ceramics, Building Materials, Dept. Biomaterials Group, Inorganic and Mineral Resources Division, National Research Centre, Egypt. Electronic address: wafaaghareib@gmail.com. 2. Spectroscopy Dept., Physics Division, National Research Centre, Egypt. 3. Water Pollution Research Dept., Environmental Division, National Research Centre, Egypt. 4. Refractories, Ceramics, Building Materials, Dept. Biomaterials Group, Inorganic and Mineral Resources Division, National Research Centre, Egypt.
Abstract
INTRODUCTION: Thermo-responsive hydrogels are promising biomedical systems as their gelation is triggered by temperature changes. Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine. MATERIALS AND METHODS: Chitosan/phosphate thermosensitive gels were successfully achieved. The developed composite scaffolds were functionalized with the greenly synthesized Ag or Ag@Pd targeting improved bactericidal activity and biocompatibility performance. The physicochemical characterization was assessed through TGA, DSC, FESEM, HRTEM, XRD and FTIR. Bactericidal activities were tested against gram- positive Staphylococcus aureus and gram-negative Pseudomonas aeruginosa. Their biodegradability upon DMEM immersion was followed up to seven days through measuring ionic concentrations of Ca, P, Ag and Pd successively. KEY FINDINGS: The newly developed phosphatic layers over the scaffold surfaces post-immersion assessed their osteogenic ability. Further, their promising and differentiated bactericidal activities due to the noble metals incorporation were proved. Cytotoxicity assessment demonstrated their high biocompatibility since no toxic effect was recorded. SIGNIFICANCE: Consequently, they can be successfully and directly applied in biomedical and dental surgeries.
INTRODUCTION: Thermo-responsive hydrogels are promising biomedical systems as their gelation is triggered by temperature changes. Greenly synthesized noble metallic nanoparticles are a growing research area assessing their potential applications in nanomedicine. MATERIALS AND METHODS: Chitosan/phosphate thermosensitive gels were successfully achieved. The developed composite scaffolds were functionalized with the greenly synthesized Ag or Ag@Pd targeting improved bactericidal activity and biocompatibility performance. The physicochemical characterization was assessed through TGA, DSC, FESEM, HRTEM, XRD and FTIR. Bactericidal activities were tested against gram- positive Staphylococcus aureus and gram-negative Pseudomonas aeruginosa. Their biodegradability upon DMEM immersion was followed up to seven days through measuring ionic concentrations of Ca, P, Ag and Pd successively. KEY FINDINGS: The newly developed phosphatic layers over the scaffold surfaces post-immersion assessed their osteogenic ability. Further, their promising and differentiated bactericidal activities due to the noble metals incorporation were proved. Cytotoxicity assessment demonstrated their high biocompatibility since no toxic effect was recorded. SIGNIFICANCE: Consequently, they can be successfully and directly applied in biomedical and dental surgeries.
Authors: Mohamed E El-Hefnawy; Sultan Alhayyani; Mohsen M El-Sherbiny; Mohamed I Sakran; Mohamed H El-Newehy Journal: Polymers (Basel) Date: 2022-01-13 Impact factor: 4.329