Fabrizio Bertelloni1, Giovanni Tosi2, Paola Massi2, Laura Fiorentini2, Maria Parigi2, Domenico Cerri3, Valentina Virginia Ebani3. 1. Department of Veterinary Science, University of Pisa, Viale Delle Piagge 2, 56124 Pisa Italy. Electronic address: fabriziobertelloni@gmail.com. 2. Istituto Zooprofilattico Sperimentale Della Lombardia e Dell'Emilia Romagna, Sezione Diagnostica di Forlì, Via Don E. Servadei 3E/3F, 47122 Forlì (FC) Italy. 3. Department of Veterinary Science, University of Pisa, Viale Delle Piagge 2, 56124 Pisa Italy.
Abstract
OBJECTIVE: To investigate some pathogenic characters of Salmonella enterica strains isolated from poultry. METHODS: Twenty-three genetically distinct Salmonella enterica strains, of different serovars and pulsotype, were examined for virulence traits. Resistance to gastric acid environment was estimated by measuring the percentage of survived bacterial cells after exposure for 2 h to a synthetic gastric juice. Strains were analyzed with PCR for the presence of the following virulence genes: mgtC and rhuM located on SPI-3, sopB and pipB located on SPI-5, Salmonella virulence plasmid (spv) R (spvR), spvB and spvC located on Salmonella plasmid virulence and sodCI, sopE, and gipA located on prophage. Finally, resistance to 21 antibiotics was tested with Kirby-Bauer method. RESULTS: A percentage of 82.60% of strains were resistant to gastric environment after induction and 60.87% of the strains exhibited constitutive resistance too. Nineteen different virulence profiles were detected. The phage related genes sodCI and sopE and the plasmid mediated operon spvR, spvB and spvC (spvRBC) were detected in 82.60%, 47.82% and 52.17% of strains, respectively. Typhimurium and Enteritidis strains showed the highest number of virulence genes. Twenty-one different antibiotic resistance profiles were obtained and two isolates (Typhimurium and Enteritidis) resulted sensible to all the tested molecules. The ampicillin, streptomycin, sulfonamide and tetracycline resistance profile was detected in seven isolates (30.43%). CONCLUSION: Our results show that paratyphoid Salmonella strains with several characters of pathogenicity, that may be cause of severe pathology in animals and humans, are circulating among poultry.
OBJECTIVE: To investigate some pathogenic characters of Salmonella enterica strains isolated from poultry. METHODS: Twenty-three genetically distinct Salmonella enterica strains, of different serovars and pulsotype, were examined for virulence traits. Resistance to gastric acid environment was estimated by measuring the percentage of survived bacterial cells after exposure for 2 h to a synthetic gastric juice. Strains were analyzed with PCR for the presence of the following virulence genes: mgtC and rhuM located on SPI-3, sopB and pipB located on SPI-5, Salmonella virulence plasmid (spv) R (spvR), spvB and spvC located on Salmonella plasmid virulence and sodCI, sopE, and gipA located on prophage. Finally, resistance to 21 antibiotics was tested with Kirby-Bauer method. RESULTS: A percentage of 82.60% of strains were resistant to gastric environment after induction and 60.87% of the strains exhibited constitutive resistance too. Nineteen different virulence profiles were detected. The phage related genes sodCI and sopE and the plasmid mediated operon spvR, spvB and spvC (spvRBC) were detected in 82.60%, 47.82% and 52.17% of strains, respectively. Typhimurium and Enteritidis strains showed the highest number of virulence genes. Twenty-one different antibiotic resistance profiles were obtained and two isolates (Typhimurium and Enteritidis) resulted sensible to all the tested molecules. The ampicillin, streptomycin, sulfonamide and tetracycline resistance profile was detected in seven isolates (30.43%). CONCLUSION: Our results show that paratyphoid Salmonella strains with several characters of pathogenicity, that may be cause of severe pathology in animals and humans, are circulating among poultry.
Authors: Daniel Hernandez-Patlan; Bruno Solis-Cruz; Karine Patrin Pontin; Juan D Latorre; Mikayla F A Baxter; Xochitl Hernandez-Velasco; Ruben Merino-Guzman; Abraham Méndez-Albores; Billy M Hargis; Raquel Lopez-Arellano; Guillermo Tellez Journal: Front Microbiol Date: 2018-06-15 Impact factor: 5.640
Authors: Manal A M Mahmoud; Gaber Megahed; Mohamed S Yousef; Fatma Abo Zakaib Ali; Rania S Zaki; Hanan H Abdelhafeez Journal: Pathogens Date: 2020-04-24