Degarelix treatment is compatible with diabetes and antithrombotic therapy in patients with prostate cancer.

INTRODUCTION: Therapeutically induced androgen deficiency (AD) is a standard treatment for patients with prostate cancer, but it is often associated with various adverse effects (AEs) that may lead to discontinuation. Some AEs may depend on the patient's health condition, while others may be due to complications of the drug delivery method. Degarelix is a gonadotropin-releasing hormone (GnRH) antagonist widely used for the treatment of androgen-dependent prostate cancer. This study aimed to ascertain the following: 1) the compatibility of degarelix treatment with diabetes and 2) any specific causal associations of degarelix injections with increased blood clotting and antithrombotic therapy requirements. PATIENTS AND METHODS: The medical records of 162 patients with prostate cancer who had undergone degarelix treatment were retrospectively examined. The association of a medical history of diabetes and anticoagulant co-treatment with degarelix treatment discontinuation was analyzed statistically.
RESULTS: Rapid and significant decreases in prostate-specific antigen (PSA) levels during the course of degarelix treatment were detected for patients with prostate cancer regardless of clinical state. During the 27 months of treatment, 68 subjects (48%) ceased degarelix treatment, owing to several reasons, mainly financial issues. Among these subjects, 19 had diabetes, while 35 were treated with antithrombotics. Extensive statistical analysis indicated that there were no causal associations between degarelix treatment discontinuation and preexisting diabetes or antithrombotic therapy.
CONCLUSION: Our study suggests that preexisting diabetes and antithrombotic therapy were not significant factors for the discontinuation of degarelix treatment in patients with prostate cancer.

Introduction

Induction of androgen deficiency (AD) is the most commonly used treatment for patients with advanced/metastatic prostate cancer, since the development of prostate cancer is dependent on activated androgen signaling.1,2 An AD state is achievable surgically (by bilateral orchidectomy) or therapeutically (by blunting androgen production via gonadotropin-releasing hormone [GnRH]-facilitated luteinizing hormone [LH] and follicle-stimulating hormone [FSH; ie, GnRH-LH/FSH axis]) to debilitate androgen receptor function.1–3 Furthermore, the function of androgen receptors can be inhibited by androgen antagonists.4,5 Since GnRH is the most upstream endocrine inducer of androgen production, GnRH agonists were first developed for AD therapy to establish a negative feedback regulation of androgen production.6,7 However, the potential for non-physiological rapid increases in testosterone levels in patients treated with GnRH agonists, leading to androgen receptor activation in prostate tumors and further tumor growth stimulation, is a significant concern.8,9 GnRH antagonists thus emerged to achieve the AD state without detrimental testosterone surges and consequent increases in serum prostate-specific antigen (PSA) levels in treated patients.10–12 Currently, one GnRH antagonist, degarelix, is widely used for AD induction therapy and has been proven to be at least as efficient as some known GnRH and LH agonists by many indicators of clinical efficacy, but without their undesirable side effects.13,14

Similar to other hormone agonists and antagonists indicated for AD therapy, degarelix is also known to cause complications, mainly related to AD.13,14 Therapeutically induced AD is commonly associated with typical adverse effects (AEs) such as hot flushes, fatigue and weight gain, regardless of the specific therapeutic compound administered.1,2 As the physiological actions of androgens facilitate basal metabolism in males via energy expenditure more efficiently than in females,15,16 AD therapy has been suggested to be risky in patients with diabetes. Thus, AD therapy has been believed to possibly lead to undesired health complications in treated prostate cancer patients.1–3 Particularly, the occurrence of severe AEs often deters patients from continuing therapy, leading to discontinuation. Specific AEs have also been associated with degarelix, presumably related to the route of administration.17–20 As degarelix is given by repeated injections, injection site reactions, such as pain, erythema, swelling and nodules, have been noted as AEs. Since severe injection site skin reactions and related complications may force patients to discontinue ongoing therapy,18,19 it is beneficial for the patients with prostate cancer to minimize any baseline risk that may contribute to the occurrence of undesired AEs, including injection site reactions.

Skin is prone to hypersensitivity in patients with certain conditions and/or drug treatments for diseases other than prostate cancer. Patients with diabetes are highly susceptible to skin complications, such as bacterial/fungal infections, severe irritation and itching.21,22 Such clinical preconditions could be serious confounding factors for treatments requiring repeated injections. Antithrombotic treatment is also often used for elderly patients, including prostate cancer patients, and may lead to eventual bleeding.23,24 As serious AEs, including skin complications induced by repeated degarelix injections, lead to discontinuation of therapy,18,19 the current retrospective study was undertaken to assess whether diabetes and/or antithrombotic treatment were risk factors for discontinuation of degarelix therapy in patients with prostate cancer with a Gleason score higher than 6.

Patients and methods

Data sources

We retrospectively examined the records of 162 patients with prostate cancer treated with degarelix at the Jyoban Hospital (Iwaki, Japan) from November 2012 to October 2015. The subjects were classified into four subgroups, three of which depended on Gleason scores and serum PSA levels (ie, D’Amico’s method that stratifies patients with localized prostate cancer into low, intermediate or high risk of biochemical recurrence)25 and one subgroup of patients with advanced prostate cancer with metastasis to other organs. The patients with advanced prostate cancer bearing metastatic tumors were also treated with other drugs, such as docetaxel, enzalutamide, or abiraterone, to inhibit further cancer cell proliferation.

Degarelix was periodically injected subcutaneously according to the recommended protocol: 240 mg for the first injection and then 80 mg every month. We assessed baseline characteristics, ongoing and past therapy, and switches to other drugs and therapy. To evaluate the treatment response, we monitored the serum PSA levels (ng/mL).

Diabetes mellitus was diagnosed according to the criteria of the Japanese Diabetes Society.26

Antithrombotic therapy included the following drugs: antiplatelet agents (eg, aspirin, clopidogrel, cilostazol, ticlopidine, eicosapentaenoic acid, and limaprost alfadex) and anticoagulants with vitamin K inhibition (eg, warfarin) or direct factor Xa inhibition (eg, rivaroxaban).27

Statistical analysis

To evaluate the crude relationship between having diabetes and receiving an antithrombotic treatment and discontinuation of degarelix therapy, we considered the Kaplan–Meier product limit method.26,27 The Kaplan–Meier method is a widely used retrospective technique to estimate the cumulative probability of event occurrence (ie, discontinuation of degarelix therapy) at a particular follow-up time, which is also known as a probability curve. Using the Wilcoxon test and the log-rank test,28,29 we compared the estimated probability curves between subjects with or without diabetes and between those who received or did not receive antithrombotic treatment.

A Cox proportional hazards regression model – one of the most common methods used in time-to-event data analysis28,29 – was also employed to evaluate the relationship between having diabetes and receiving an antithrombotic treatment and discontinuation of degarelix therapy, with adjustment for covariates. Independent variables considered in the model were prostate cancer subgroups (low, intermediate or high risk of recurrence in localized prostate cancer according to D’Amico risk stratification, or metastatic cancer), age at diagnosis, initial pretreatment PSA level (ng/mL) and experience of radical prostatectomy.

All statistical analyses were conducted using STATA/IC 14 (StataCorp LLC, College Station, TX, USA), and a p-value of less than 0.05 was considered statistically significant. The datasets are available on reasonable request to the corresponding authors.

Ethics

This study underwent ethical review and was approved by the Jyoban Hospital Institutional Review Board. All study participants provided written informed consent.

Results

Patient characteristics

The baseline characteristics of the studied patients are summarized in Table 1. Among the 162 subjects, 114 subjects with localized prostate cancer were classified into subgroups of low (n=14), intermediate (n=31) and high risk (n=69) of recurrence, based on Gleason scores and serum PSA levels, and the remaining subjects (n=48) had metastatic tumors. The mean age and SD of the four subgroups at diagnosis were 72.1 (5.2), 74.6 (7.2), 76.6 (6.3) and 74.2 (9.5) years, respectively, which were not statistically significantly different (p=0.66: ANOVA). The minimum age was 48 years, and the maximum age was 89 years. The mean (standard deviation: SD) initial PSA levels were 6.2 (1.9), 10.4 (4.7), 31.8 (36.5) and 390.9 (360.3) ng/mL, respectively, which were statistically significantly different (p<0.001: ANOVA). Note that nine subjects with metastasis (18.8% of 48) had initial PSA levels >999 ng/mL, which were scored as “999” by default. This means that the mean initial PSA level for the metastasis group was underestimated. The Gleason scores for low- and intermediate-risk groups were within 7, but they were more than 8 for the high-risk and metastasis groups. Two cases in the high-risk group and six cases in the metastasis group had undergone prior therapies before degarelix administration.

Table 1

Patient characteristics (n=162)

Characteristics	Group
	Low risk	Intermediate risk	High risk	Metastasis
Number of cases	14	31	69	48
Age (years), mean (SD)	72.1 (5.2)	74.6 (7.2)	76.6 (6.3)	74.2 (9.5)
Initial PSA (ng/mL), mean (SD)	6.2 (1.9)	10.4 (4.7)	31.8 (36.5)	390.9 (360.3)
Gleason score
6	14	1	2	–
7	–	30	14	1
More than 8	–	–	54	42
Previous treatment	–	–	2	6
Combination of drug
Docetaxel	–	–	–	4
Enzalutamide	–	–	1	9
Abiraterone	–	–	–	4

Abbreviation: PSA, prostate-specific antigen.

The PSA response to degarelix therapy is shown in Figure 1. In the three subgroups of patients with localized prostate cancer, the PSA levels decreased sharply in the first 3 months, and gradual declines continued over 1 year. Up to the assessed 27 months, the PSA levels were maintained at normal levels (about 4 ng/mL) for these three subgroups. Degarelix therapy was also effective for the group with metastasis, as a similar sharp drop in PSA levels was also detectable in the first 3 months, but they remained high (more than 4 ng/mL), even when assessed after 27 months of treatment.

Figure 1

PSA response to degarelix therapy in the (A) low-risk group, (B) intermediate-risk group, (C) high-risk group and (D) metastasis group.

Abbreviation: PSA, prostate-specific antigen.

Discontinuation of degarelix use

Nearly half of all subjects (n=68: 42.0%) ceased degarelix use. The reasons for discontinuation of degarelix therapy are summarized in Table 2. The most common causes of discontinuation were not serious AEs, but switches to the other drugs (n=12: 7.4%), or due to drugs not being effective (n=10: 6.2%). Nine patients (5.6%) discontinued degarelix because of AEs, including five (3.1%) severe injection site reactions.

Table 2

Summary of reasons for discontinuation of degarelix therapy

Reasons	Group
	Low risk	Intermediate risk	High risk	Metastasis
Discontinuation cases/total cases	4/14	9/31	36/69	19/48
Adverse events
Injection site reaction	–	1	3	1
Hot flash	–	1	–	–
Malaise	–	1	–	1
Suspicion of hepatic disorder	–	–	1	–
Change to other drugs	–	1	11	–
Drugs ineffective	–	–	2	8
Deaths	–	–	2	1
Difficulty in attending	2	1	1	–
Others*	2	4	16	8
Period until discontinuation (day), mean (SD)	116.3 (139.7)	268.7 (176.1)	349.9 (204.8)	370.5 (300.0)

Note:

Others include combination with high-intensity focused ultrasound or radiotherapy.

Probability of discontinuation of degarelix use

We then assessed if an association existed between other clinical conditions and degarelix therapy discontinuation. Although injection site reaction AEs were not raised as major reasons for discontinuation, diabetes and antithrombotic treatment might have exerted an influence on enhancing serious AEs that eventually led to discontinuation of degarelix therapy. Figure 2 shows the Kaplan–Meier curves of the probability of discontinuation of degarelix therapy. The probability was plotted separately in subjects with or without diabetes (the upper graph in Figure 2A) and in those who received or did not receive an antithrombotic treatment (the lower graph in Figure 2B), against the analysis time that started from the date of the initial administration of degarelix.

Figure 2

Estimated probability of discontinuation of degarelix therapy by diabetes status (A) and anticoagulant treatment conditions (B).

No significant difference in probability curves for discontinuation of degarelix therapy was observed between subjects with or without diabetes and those who received or did not receive an antithrombotic treatment. The p-values from the Wilcoxon test were 0.54 and 0.35, respectively, and from the log-rank test wre 0.44 and 0.23, respectively.

Regression model for discontinuation of degarelix use

The results of the multiple Cox proportional hazards regression model are summarized in Table 3 and are expressed as a multiplicative change (ie, hazard ratio) in the likelihood of discontinuation of degarelix therapy for a unit increase/change in the independent variable.

Table 3

Multiple Cox regression model showing the effect of being diabetic and receiving an antithrombotic treatment on discontinuation of degarelix therapy

Variable	Hazard ratio	95% CI	p-value
Diabetes status
No	1.00
Yes	1.19	0.51–2.75	0.69
Antithrombotic treatment
No	1.00
Yes	1.63	0.89–2.98	0.11
Cancer risk group
Low risk	0.79	0.14–4.33	0.78
Intermediate risk	1.33	0.48–3.76	0.58
High risk	1.42	0.62–3.24	0.41
Metastasis	1.00
Age at diagnosis	0.99	0.95–1.03	0.49
Initial PSA levels (per 1 ng/mL)	1.00	1.00–1.00	0.46
Radical prostatectomy surgery
Not experienced	1.00
Experienced	4.69	2.32–9.49	<0.001

Abbreviation: PSA, prostate-specific antigen.

The model that we specified is the following: where h(t) is the hazard function, h0(t) is the baseline hazard function at time t and exp(β) indicates the hazard ratio. The model incorporated six covariates (X1: diabetes status – no or yes; X2: antithrombotic treatment conditions – no or yes; X3: cancer risk levels – low, intermediate or high risk of recurrence for localized cancer, or metastatic cancer; X4: age at diagnosis; X5: initial PSA levels and X6: radical prostatectomy – not experienced or experienced). Our major interests were comparisons between “no” and “yes” with respect to the covariates “diabetes status” and “antithrombotic treatment,” effects that were expressed as β1 and β2, respectively. This model takes into account the time from the date of the initial administration of degarelix until its discontinuation or the last date of follow-up.

After adjusting for covariates, having diabetes and receiving an antithrombotic treatment had no significant relationship with discontinuation of degarelix therapy, with hazard ratios of 1.19 (95% CI 0.51–2.75, p=0.69) and 1.63 (95% CI 0.89–2.98, p=0.11), respectively. Radical prostatectomy was the only variable significantly associated with discontinuation of degarelix therapy (hazard ratio 4.69, 95% CI 2.32–9.49, p<0.001).

Discussion

GnRH antagonists were developed to overcome the complications of the GnRH agonists that caused a rapid surge of androgen production and consequent exacerbation of prostate tumors.13,14 Numerous clinical studies have confirmed that a GnRH antagonist, degarelix, successfully induced an AD state without a transient increase in serum testosterone levels, and with a robust decrease in serum PSA levels in patients with prostate cancer of different stages.10–12 Consistently, in the current study, the expected PSA response was seen in patients of all subgroups. Since the serum PSA level is a better clinical marker for prostate cancer therapy than serum testosterone levels, serum testosterone levels were not systemically assessed in the treated patients. However, it should be noted that monitoring serum testosterone levels in patients with prostate cancer can also be very informative to predict cancer progression, as endogenous production of androgens is dysregulated during the treatments.30–32 Previous studies suggested that repeated degarelix injections might provoke skin reactions at the injection site, particularly at the first injection.10–12 It has been estimated that injection site reactions occur in over 40% of degarelix-treated patients11 and that they eventually become serious enough to cause discontinuation of therapy. In the current study, we also observed that large numbers of patients developed injection site reactions of varying severity, but they only caused discontinuation of degarelix therapy in four cases. Thus, degarelix therapy may cause injection site reactions, but they may not be serious enough to prompt discontinuation from the perspective of both patients and doctors.

Previous reports of frequent AEs among treated patients led us to question whether these serious AEs resulted in discontinuation in our study, as the major causes had been potentiated by the preexisting conditions of the treated patients. We focused specifically on patients with documented clinical conditions of higher susceptibility to any skin stimulation, since their skin conditions would be likely to deteriorate in response to regular injections, leading to potentially serious AEs in skin and eventual treatment discontinuation. Although a high frequency of injection site reactions has been observed in many studies,10–12 information about clinical associations between the occurrence of the injection site reactions and other disease conditions is scarce. We particularly focused on common diseases and treatments that might potentiate the occurrence of AEs during degarelix therapy. In this study, we assessed whether there was an association between discontinuation of degarelix therapy and diabetes, since the skin in patients with diabetes is known to be hypersensitive to any stimulation21,22 and, therefore likely to develop serious AEs resulting in discontinuation of any non-vital treatment. Similarly, co-treatment with antithrombotic agents is also assumed to potentiate the occurrence of AEs in response to degarelix injections, since antithrombotic treatment is well known to often cause side effects related to bleeding in the skin and other organs.23,24

Nevertheless, a relationship between diabetes and the incidence of AEs related to degarelix therapy was not found. Similarly, a causal association of AEs with degarelix therapy and antithrombotic treatment remains to be ascertained. There were 19 subjects among the tested 148 subjects with prostate cancer who were clinically diabetic. We could determine no statistical association between degarelix treatment discontinuation and diabetes in this group of patients (Figure 2 and Table 3). Thus, the incidence of common AEs, including injection site reactions, is unlikely to be influenced by diabetes. However, the relationship between AEs and body mass index (BMI) still remains to be tested, due to lack of the BMI data for this cohort, as local androgen actions for cancer progression have been indicated to be potentiated by the increased adipocyte tissues.3,33 Likewise, under the same statistical analyses, antithrombotic co-treatment was not causal for discontinuation of degarelix therapy (Figure 2 and Table 3). At the same time, we observed a statistically significant association of degarelix therapy discontinuation with radical prostatectomy, but the basis of this association remains to be addressed in further studies.

In this study, we analyzed the potential for a causal association between degarelix therapy discontinuation and the most common preexisting condition (diabetes) or co-treatment (antithrombotic therapy) in a cohort of prostate cancer patients. It is possible that other common diseases and co-therapies may lead to the development of different serious AEs that would eventually lead to discontinuation of degarelix therapy. To address this issue, further studies on larger cohorts of subjects are clearly required to optimize therapeutic strategies for degarelix treatment in patients with prostate cancer to avoid undesired AEs and unnecessary discontinuation of treatment.

The limitations of this study are the very small number of subjects as well as its retrospective nature. Although a recent paper reported a genetic effect by polymorphism in the gene locus of aldo-keto reductase 1C3,34 we were unable to assess whether genetic variations were associated with the AEs. The diabetic condition and antithrombotic therapy among the studied patients were diverse, but for the degarelix effect assessment, the patients were simply classified into four groups based on the prostate cancer states evaluated by D’Amico’s method.

Conclusion

Degarelix effectively induced an AD state in patients with prostate cancer, and frequent occurrence of AEs was observed, in accordance with past reports. However, preexisting diabetes and antithrombotic therapy were not statistically significant predictors for the discontinuation of degarelix treatment in patients with prostate cancer.