Mei Fu1,2,3, Wen-Ming Xu4, Tai-Zhou Qin5, Ke-Hui Xu4,2. 1. Department of Reproductive Endocrinology,Joint Laboratory for Reproductive Medicine, SCU-CUHK,West China Second University Hospital,Sichuan University,Chengdu 610041,China. 2. Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University) ,Ministry of Education,Chengdu 610041,China. 3. Department of Obstetrics and Gynecology,Chengdu Women and Children's Central Hospital, Chengdu 610041, China. 4. Department of Reproductive Endocrinology,Joint Laboratory for Reproductive Medicine, SCU-CUHK,West China Second University Hospital, Sichuan University,Chengdu 610041,China. 5. Department of Obstetrics and Gynecology,Chengdu Women and Children's Central Hospital,Chengdu 610041,China.
Abstract
OBJECTIVE: To explore the association between polymorphisms of miRNA biogenesis related gene DICER,DROSHA,RAN and unexplained recurrent spontaneous abortion (URSA) in Chinese women. METHODS: We recruited 217 patients with URSA (URSA group) and 390 healthy controls who were fertile women with history of more than one successful pregnancy outcome (control group) from June 2013 to December 2015 in the West China Second University Hospital of Sichuan University. The control group was recruited from regular physical examination and prepregnancy check for women of childbearing age during the same period. A case-control study was performed to analyze polymorphism of miRNA machinery genes,including DICER rs3742330,DROSHA rs10719, RAN rs14035,by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. RESULTS: The frequencies of genotypes and alleles of DICER rs3742330,DROSHA rs10719,and RAN rs14035 showed no difference between URSA patients and control group (all P>0.05). DICER rs3742330/DROSHA rs10719 GG/TC+TT combinations were synergistically associated with increased URSA risk [odds ratio (OR)=1.657,95% confidence interval (CI)=1.006-2.731, P=0.047]. Although DICER rs3742330/RAN rs14035 GG / TT+TC combinations was significantly higher in the URSA group than in the control group,there was no statistical significance (OR=1.977,95%CI=0.956-4.087, P=0.066). However,DROSHA10719/RAN14035 TT+TC/TT+TC had no significant correlation with URSA (OR=0.958,95%CI=0.679-1.353, P=0.808). CONCLUSION: Our study demonstrated the relationship between URSA development and combined genotype of DICER rs3742330/DROSHA rs10719 GG/TC+TT.
OBJECTIVE: To explore the association between polymorphisms of miRNA biogenesis related gene DICER,DROSHA,RAN and unexplained recurrent spontaneous abortion (URSA) in Chinese women. METHODS: We recruited 217 patients with URSA (URSA group) and 390 healthy controls who were fertile women with history of more than one successful pregnancy outcome (control group) from June 2013 to December 2015 in the West China Second University Hospital of Sichuan University. The control group was recruited from regular physical examination and prepregnancy check for women of childbearing age during the same period. A case-control study was performed to analyze polymorphism of miRNA machinery genes,including DICER rs3742330,DROSHArs10719, RANrs14035,by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. RESULTS: The frequencies of genotypes and alleles of DICER rs3742330,DROSHArs10719,and RANrs14035 showed no difference between URSA patients and control group (all P>0.05). DICER rs3742330/DROSHArs10719 GG/TC+TT combinations were synergistically associated with increased URSA risk [odds ratio (OR)=1.657,95% confidence interval (CI)=1.006-2.731, P=0.047]. Although DICER rs3742330/RANrs14035 GG / TT+TC combinations was significantly higher in the URSA group than in the control group,there was no statistical significance (OR=1.977,95%CI=0.956-4.087, P=0.066). However,DROSHA10719/RAN14035 TT+TC/TT+TC had no significant correlation with URSA (OR=0.958,95%CI=0.679-1.353, P=0.808). CONCLUSION: Our study demonstrated the relationship between URSA development and combined genotype of DICER rs3742330/DROSHArs10719 GG/TC+TT.
Authors: Yubin Lee; Eun Hee Ahn; Chang Soo Ryu; Jung Oh Kim; Hui Jeong An; Sung Hwan Cho; Ji Hyang Kim; Young Ran Kim; Woo Sik Lee; Nam Keun Kim Journal: Exp Ther Med Date: 2020-02-26 Impact factor: 2.447