Hideaki Watanabe1,2, Takuya Matsumoto2, Moeka Nishi2, Ken Takeshi Kusakabe3, Takeshi Kuraishi4, Shosaku Hattori4, Hiromichi Matsumoto1,2, Emiko Fukui1,2, Akiko Kuwahata5, Masanori Ochi5, Yasuo Kiso3, Chieko Kai6, Midori Yoshizawa1,2. 1. United Graduate School of Agricultural Science Tokyo University of Agriculture and Technology 183-8509 Fuchu-shi Japan. 2. Graduate School of Agricultural Science Utsunomiya University 321-8505 Tochigi Japan. 3. Joint Faculty of Veterinary Science Yamaguchi University 753-8515 Yamaguchi Japan. 4. Amami Laboratory, Institute of Medical Science The University of Tokyo 894-1531 Kagoshima Japan. 5. Ochi Yume Clinic 460-0002 Nagoya Aichi Japan. 6. Institute of Medical Science The University of Tokyo 108-8639 Tokyo Japan.
Abstract
Purpose: We explored the possibility of employing intracytoplasmic sperm injection (ICSI), involving oocytes and sperm of owl monkeys, to increase the availability of this species for investigations relating to malaria, etc., by increasing the number of animals in our laboratory. Methods: Two owl monkeys (a female and a male), raised at the Amami Laboratory of the University of Tokyo, were used. Follicular oocytes surrounded with cumulus cells were cultured in vitro for approximately 25 h and cumulus cells were removed with 0.1 % hyaluronidase. Because of the poor motility of caudal epididymal sperm, sperm were injected without adding polyvinylpyrrolidone to immobilize them. The ICSI procedure was performed by an individual with considerable experience of human ICSI. Results: We were able to produce two owl monkey embryos using ICSI of oocytes that matured to MII stage. Both embryos reached the 10-cell stage at 98 h after ICSI and showed signs of compaction, but failed to cleave further. Conclusions: Although we successfully produced owl monkey embryos after ICSI, the embryos did not develop to the blastocyst stage. Many parameters need to be studied further, including superovulation, selection of culture media, and selection of good quality sperm in order to achieve successful ICSI in the owl monkey.
Purpose: We explored the possibility of employing intracytoplasmic sperm injection (ICSI), involving oocytes and sperm of owl monkeys, to increase the availability of this species for investigations relating to malaria, etc., by increasing the number of animals in our laboratory. Methods: Two owl monkeys (a female and a male), raised at the Amami Laboratory of the University of Tokyo, were used. Follicular oocytes surrounded with cumulus cells were cultured in vitro for approximately 25 h and cumulus cells were removed with 0.1 % hyaluronidase. Because of the poor motility of caudal epididymal sperm, sperm were injected without adding polyvinylpyrrolidone to immobilize them. The ICSI procedure was performed by an individual with considerable experience of human ICSI. Results: We were able to produce two owl monkey embryos using ICSI of oocytes that matured to MII stage. Both embryos reached the 10-cell stage at 98 h after ICSI and showed signs of compaction, but failed to cleave further. Conclusions: Although we successfully produced owl monkey embryos after ICSI, the embryos did not develop to the blastocyst stage. Many parameters need to be studied further, including superovulation, selection of culture media, and selection of good quality sperm in order to achieve successful ICSI in the owl monkey.
Authors: Lourdes Muriel; Nicolás Garrido; José Luis Fernández; José Remohí; Antonio Pellicer; Maria José de los Santos; Marcos Meseguer Journal: Fertil Steril Date: 2006-02 Impact factor: 7.329