Sullim Lee1, No-June Park2, Sim-Kyu Bong3, Jonghwan Jegal4, Sang-A Park5, Su-Nam Kim6, Min Hye Yang7. 1. Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea. Electronic address: sullimlee@kist.re.kr. 2. Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea. Electronic address: 115519@kist.re.kr. 3. Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea. Electronic address: 115044@kist.re.kr. 4. College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea. Electronic address: jhjegal@pusan.ac.kr. 5. College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea. Electronic address: sapark@live.co.kr. 6. Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea. Electronic address: snkim@kist.re.kr. 7. College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea. Electronic address: mhyang@pusan.ac.kr.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: The fruits of Juniperus rigida have been used in Korean traditional medicine for the treatment of inflammatory diseases in humans such as rheumatoid arthritis. AIM OF THE STUDY: This study aimed to investigate the anti-atopic properties of J. rigida fruit in in vivo murine atopic dermatitis (AD) models. METHODS AND RESULTS: BALB/c mouse ears ad SKH-1 hairless mice stimulated with oxazolone (4 weeks) and DNCB (3 weeks), respectively, were treated with the 1% Juniperus rigida fruit EtOH extract (JFE). The JFE improved AD symptoms in both oxazolone- and DNCB-induced AD mice by accelerating skin barrier recovery function and suppressing the overproduction of serum immunoglobulin E (IgE) and interleukin 4 (IL-4). The JFE was found to contain isoscutellarein-7-O-β-xylopyranoside, cupressuflavone, podocarpusflavone A, and hinokiflavone as major components based on phytochemical analysis. Eight flavonoids were isolated from JFE, and of those, cupressuflavone and isoscutellarein-7-O-β-xylopyranoside strongly down-regulated IL-4 expression and β-hexosaminidase release in RBL-2H3 cells. CONCLUSION: Therapeutic attempts with J. rigida fruit and its active components might be useful in treating AD and related skin inflammatory diseases.
ETHNOPHARMACOLOGICAL RELEVANCE: The fruits of Juniperus rigida have been used in Korean traditional medicine for the treatment of inflammatory diseases in humans such as rheumatoid arthritis. AIM OF THE STUDY: This study aimed to investigate the anti-atopic properties of J. rigida fruit in in vivo murineatopic dermatitis (AD) models. METHODS AND RESULTS: BALB/c mouse ears ad SKH-1 hairless mice stimulated with oxazolone (4 weeks) and DNCB (3 weeks), respectively, were treated with the 1% Juniperus rigida fruit EtOH extract (JFE). The JFE improved AD symptoms in both oxazolone- and DNCB-induced ADmice by accelerating skin barrier recovery function and suppressing the overproduction of serum immunoglobulin E (IgE) and interleukin 4 (IL-4). The JFE was found to contain isoscutellarein-7-O-β-xylopyranoside, cupressuflavone, podocarpusflavone A, and hinokiflavone as major components based on phytochemical analysis. Eight flavonoids were isolated from JFE, and of those, cupressuflavone and isoscutellarein-7-O-β-xylopyranoside strongly down-regulated IL-4 expression and β-hexosaminidase release in RBL-2H3 cells. CONCLUSION: Therapeutic attempts with J. rigida fruit and its active components might be useful in treating AD and related skin inflammatory diseases.
Authors: Vivi Nur Khalieda Mohd Kasim; Siti Mahirah Noble; Kong Yen Liew; Ji Wei Tan; Daud Ahmad Israf; Chau Ling Tham Journal: Front Pharmacol Date: 2022-05-24 Impact factor: 5.988