L Paiva1, T K S Fidalgo2, L P da Costa3, L C Maia4, L Balan5, K Anselme5, L Ploux5, R M S M Thiré6. 1. Université de Haute-Alsace, CNRS, IS2M UMR 7361, F-68100 Mulhouse, France; Université de Strasbourg, France; Program of Metallurgical and Materials Engineering, COPPE, Federal University of Rio de Janeiro -UFRJ, Rio de Janeiro, RJ, Brazil. Electronic address: paiva.lilian@outlook.com. 2. Preventive and Community Dentistry, School of Dentistry, State University of Rio de Janeiro - UERJ, Rio de Janeiro, RJ, Brazil. 3. Graduate Program in Industrial Biotechnology, Tiradentes University -UNIT, Aracaju, SE, Brazil. 4. Department of Pediatric Dentistry and Orthodontics, School of Dentistry, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, RJ, Brazil. 5. Université de Haute-Alsace, CNRS, IS2M UMR 7361, F-68100 Mulhouse, France; Université de Strasbourg, France. 6. Program of Metallurgical and Materials Engineering, COPPE, Federal University of Rio de Janeiro -UFRJ, Rio de Janeiro, RJ, Brazil.
Abstract
OBJECTIVES: This work aimed (1) to develop polyacid formulations by the one-step photoreduction of silver nanoparticles (AgNP) in a polyacrylate solution of conventional glass ionomer cement (GIC), imparting antibacterial activity; and (2) to evaluate handling and mechanical properties of experimental ionomers in comparison to a commercially available conventional GIC. METHODS: Formulations with increasing sub-stoichiometric amounts of AgNO3 were monitored during continuous UV light exposure by UV-vis spectroscopy and analyzed by transmission electron microscopy. The resulted synthesis of formulations containing small and disperse spherical nanoparticles (∼6 nm) were used to design the experimental nano-silver glass ionomer cements (NanoAg-GIC). The cements were characterized as to net setting time and compressive strength according to ISO 9917-1:2007 specifications. The antibacterial activity of these cements was assessed by Ag+ diffusion tests on nutritive agar plates (E. coli) and by MTT assay (S. mutans). RESULTS: The higher concentration of silver (0.50% by mass) in the matrix of NanoAg-GIC allowed viable net setting time and increased in 32% compressive strength of the experimental cement. All groups containing AgNP induced statistically significant E. coli growth inhibition zones (p-value <.05), indicating diffusion of Ag+ ions on the material surroundings. Metabolic activity of S. mutans grown on NanoAg-GIG with higher concentration of silver was significantly affected compared to control (p-value <.01). CONCLUSIONS: Silver nanoparticles one-step preparation in polyacrylate solution allowed the production of highly bioactive water-based cements within suitable parameters for clinical use and with large potential of dental and biomedical application.
OBJECTIVES: This work aimed (1) to develop polyacid formulations by the one-step photoreduction of silver nanoparticles (AgNP) in a polyacrylate solution of conventional glass ionomer cement (GIC), imparting antibacterial activity; and (2) to evaluate handling and mechanical properties of experimental ionomers in comparison to a commercially available conventional GIC. METHODS: Formulations with increasing sub-stoichiometric amounts of AgNO3 were monitored during continuous UV light exposure by UV-vis spectroscopy and analyzed by transmission electron microscopy. The resulted synthesis of formulations containing small and disperse spherical nanoparticles (∼6 nm) were used to design the experimental nano-silver glass ionomer cements (NanoAg-GIC). The cements were characterized as to net setting time and compressive strength according to ISO 9917-1:2007 specifications. The antibacterial activity of these cements was assessed by Ag+ diffusion tests on nutritive agar plates (E. coli) and by MTT assay (S. mutans). RESULTS: The higher concentration of silver (0.50% by mass) in the matrix of NanoAg-GIC allowed viable net setting time and increased in 32% compressive strength of the experimental cement. All groups containing AgNP induced statistically significant E. coli growth inhibition zones (p-value <.05), indicating diffusion of Ag+ ions on the material surroundings. Metabolic activity of S. mutans grown on NanoAg-GIG with higher concentration of silver was significantly affected compared to control (p-value <.01). CONCLUSIONS:Silver nanoparticles one-step preparation in polyacrylate solution allowed the production of highly bioactive water-based cements within suitable parameters for clinical use and with large potential of dental and biomedical application.
Authors: Elena Ferrando-Magraner; Carlos Bellot-Arcís; Vanessa Paredes-Gallardo; José Manuel Almerich-Silla; Verónica García-Sanz; Mercedes Fernández-Alonso; José María Montiel-Company Journal: Medicina (Kaunas) Date: 2020-01-29 Impact factor: 2.430